Prevalence of JC polyomavirus large T antigen sequences among Iranian patients with central nervous system tumors

Prevalence of JC polyomavirus large T antigen sequences among Iranian patients with central... The human neurotropic JC virus (JCV) is of significant interest due to its experimental neuro- oncogenic potential. In clinical samples from human central nervous system (CNS) tumors, detection of JCV sequences suggests a possible association with CNS neoplasms, but the results are discrepant worldwide. To assess the prevalence of JCV sequences in Iranian patients with primary and metastatic CNS malignancies, a total of 58 fresh CNS tumors were examined by quantitative real-time PCR targeting the JCV large T antigen (LT-Ag) gene, and JCV DNA load was determined as viral copy number per cell. All patients were immunocompetent, and none of them had received immunosuppressive therapy before surgical operation. JC virus LT-Ag sequences were found in a total of 15 (25.9 %) out of the 58 tested samples. In primary CNS tumors, JCV sequences were identified more frequently in meningiomas (50.0 %) and schwannomas (35.7 %). In metastatic CNS tumors, JCV LT-Ag was identified in one case with brain adenocarcinoma originating from lung cancer. No statistically significant association between JCV positivity and various types of CNS malignancies was observed ( P = 0.565 ). The mean JCV LT-Ag copy number in 15 positive cases was 1.8 × 10 −4 ± 4.5 × 10 −4 copies per cell (range 1.0 × 10 −5 -1.78 × 10 −3 copies per cell). An inverse correlation between white blood cell (WBC) count and JCV copy number was observed, but this correlation was not statistically significant ( R = −0.198, P = 0.480 ). This study provides the first data on the prevalence of JCV in primary and metastatic CNS tumors from Iranian patients. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Archives of Virology Springer Journals

Prevalence of JC polyomavirus large T antigen sequences among Iranian patients with central nervous system tumors

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Publisher
Springer Vienna
Copyright
Copyright © 2015 by Springer-Verlag Wien
Subject
Biomedicine; Virology; Medical Microbiology; Infectious Diseases
ISSN
0304-8608
eISSN
1432-8798
D.O.I.
10.1007/s00705-014-2230-0
Publisher site
See Article on Publisher Site

Abstract

The human neurotropic JC virus (JCV) is of significant interest due to its experimental neuro- oncogenic potential. In clinical samples from human central nervous system (CNS) tumors, detection of JCV sequences suggests a possible association with CNS neoplasms, but the results are discrepant worldwide. To assess the prevalence of JCV sequences in Iranian patients with primary and metastatic CNS malignancies, a total of 58 fresh CNS tumors were examined by quantitative real-time PCR targeting the JCV large T antigen (LT-Ag) gene, and JCV DNA load was determined as viral copy number per cell. All patients were immunocompetent, and none of them had received immunosuppressive therapy before surgical operation. JC virus LT-Ag sequences were found in a total of 15 (25.9 %) out of the 58 tested samples. In primary CNS tumors, JCV sequences were identified more frequently in meningiomas (50.0 %) and schwannomas (35.7 %). In metastatic CNS tumors, JCV LT-Ag was identified in one case with brain adenocarcinoma originating from lung cancer. No statistically significant association between JCV positivity and various types of CNS malignancies was observed ( P = 0.565 ). The mean JCV LT-Ag copy number in 15 positive cases was 1.8 × 10 −4 ± 4.5 × 10 −4 copies per cell (range 1.0 × 10 −5 -1.78 × 10 −3 copies per cell). An inverse correlation between white blood cell (WBC) count and JCV copy number was observed, but this correlation was not statistically significant ( R = −0.198, P = 0.480 ). This study provides the first data on the prevalence of JCV in primary and metastatic CNS tumors from Iranian patients.

Journal

Archives of VirologySpringer Journals

Published: Jan 1, 2015

References

  • Chronic viral infection and primary central nervous system malignancy
    Saddawi-Konefka, R; Crawford, JR
  • Human polyomaviruses and brain tumors
    White, MK; Gordon, J; Reiss, K; Valle, L; Croul, S; Giordano, A; Darbinyan, A; Khalili, K
  • Progressive multifocal leukoencephalopathy revisited: Has the disease outgrown its name?
    Koralnik, IJ
  • Oncogenic DNA viruses
    zur Hausen, H
  • Detection of JC virus DNA fragments but not proteins in normal brain tissue
    Perez-Liz, G; Valle, L; Gentilella, A; Croul, S; Khalili, K
  • Role of cell cycle regulators in tumor formation in transgenic mice expressing the human neurotropic virus, JCV, early protein
    Krynska, B; Gordon, J; Otte, J; Franks, R; Knobler, R; DeLuca, A; Giordano, A; Khalili, K

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