Get 20M+ Full-Text Papers For Less Than $1.50/day. Start a 14-Day Trial for You or Your Team.

Learn More →

Prevalence of factor V leiden, MTHFR C677T and MTHFR A1298C polymorphisms in patients with deep vein thrombosis in Central Iran

Prevalence of factor V leiden, MTHFR C677T and MTHFR A1298C polymorphisms in patients with deep... Deep vein thrombosis (DVT) is a common disease, especially among elderly patients, which is associated with high costs of treatment and high rates of recurrence. The risk factors for venous thrombosis are primarily related to hypercoagulability, which can be genetic or acquired, or because of immobilization and venous stasis. Among relevant genetic markers are a number of common polymorphisms and mutations in the genes coding for Factor V leiden and methylenetetrahydrofolate reductase. Differential associations of these polymorphisms have been reported in different populations with DVT due to ethnic variations. However, no study has been reported with respect to these polymorphisms in DVT in Iran. Thus, the aim of the present study is to determine the prevalence of FVL, MTHFR C677T and MTHFR A1298C gene polymorphisms in patients with DVT in central Iran. In the present cross-sectional study, a total of 100 patients with first and recurrent episodes of DVT and age less than 70 years were recruited during 2016–2017. Blood sample was collected from the recruited patients and FVL mutation was screened using ARMS-PCR method, MTHFR C677T and MTHFR A1298C mutations were screened using PCR-RFLP method. The results revealed that MTHFR A1298C gene polymorphism in both homozygote and heterozygote form was found to be most frequent i.e. 77% among cases, followed by MTHFR C677T (67%) and FVL (17%). The study highlights the importance of screening of these genetic markers among patients with DVT in this region. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Molecular Biology Reports Springer Journals

Prevalence of factor V leiden, MTHFR C677T and MTHFR A1298C polymorphisms in patients with deep vein thrombosis in Central Iran

Download PDF
4 pages

Loading next page...
 
/lp/springer_journal/prevalence-of-factor-v-leiden-mthfr-c677t-and-mthfr-a1298c-LPP0YOlUlW
Publisher
Springer Journals
Copyright
Copyright © 2018 by Springer Science+Business Media B.V., part of Springer Nature
Subject
Life Sciences; Animal Biochemistry; Animal Anatomy / Morphology / Histology
ISSN
0301-4851
eISSN
1573-4978
DOI
10.1007/s11033-018-4201-0
pmid
29855758
Publisher site
See Article on Publisher Site

Abstract

Deep vein thrombosis (DVT) is a common disease, especially among elderly patients, which is associated with high costs of treatment and high rates of recurrence. The risk factors for venous thrombosis are primarily related to hypercoagulability, which can be genetic or acquired, or because of immobilization and venous stasis. Among relevant genetic markers are a number of common polymorphisms and mutations in the genes coding for Factor V leiden and methylenetetrahydrofolate reductase. Differential associations of these polymorphisms have been reported in different populations with DVT due to ethnic variations. However, no study has been reported with respect to these polymorphisms in DVT in Iran. Thus, the aim of the present study is to determine the prevalence of FVL, MTHFR C677T and MTHFR A1298C gene polymorphisms in patients with DVT in central Iran. In the present cross-sectional study, a total of 100 patients with first and recurrent episodes of DVT and age less than 70 years were recruited during 2016–2017. Blood sample was collected from the recruited patients and FVL mutation was screened using ARMS-PCR method, MTHFR C677T and MTHFR A1298C mutations were screened using PCR-RFLP method. The results revealed that MTHFR A1298C gene polymorphism in both homozygote and heterozygote form was found to be most frequent i.e. 77% among cases, followed by MTHFR C677T (67%) and FVL (17%). The study highlights the importance of screening of these genetic markers among patients with DVT in this region.

Journal

Molecular Biology Reports – Springer Journals

Published: May 31, 2018

References

  • PAI-1 4G/5G and MTHFR C677T polymorphisms increased the accuracy of two prediction scores for the risk of acute lower extremity deep vein thrombosis
    Pop, TR; Vesa, ŞC; Trifa, AP; Crişan, S; Buzoianu, AD
  • Trends in the incidence of deep vein thrombosis and pulmonary embolism: a 25-year population-based study
    Silverstein, MD; Heit, JA; Mohr, DN; Petterson, TM; O’fallon, WM; Melton, LJ
  • Estimated prevalence of venous thromboembolism in Iran: prophylaxis still an unmet challenge
    Sharif-Kashani, B; Mohebi-Nejad, A; Abooturabi, SM
  • Epidemiology of venous thromboembolism
    Heit, JA
  • The genetics of venous thromboembolism A meta-analysis involving ~ 120,000 cases and 180,000 controls
    Gohil, R; Peck, G; Sharma, P
  • 5, 10-Methylenetetrahydrofolate reductase gene variants and congenital anomalies: a HuGE review
    Botto, LD; Yang, Q
  • High prevalence of MTHFR gene A1298C polymorphism in Lebanon
    Sabbagh, AS; Mahfoud, Z; Taher, A; Zaatari, G; Daher, R; Mahfouz, RA
  • Spectrum of MTHFR gene SNPs C677T and A1298C: a study among 23 population groups of India
    Saraswathy, KN; Asghar, M; Samtani, R; Murry, B; Mondal, PR; Ghosh, PK; Sachdeva, MP
  • Deep venous thrombosis and thrombophilic mutations in western Iran: association with factor V Leiden
    Rahimi, Z; Mozafari, H; Shahriari-Ahmadi, A; Alimogaddam, K; Ghavamzadeh, A; Aznab, M; Mansouri, K; Rezaei, M; Parsian, A
  • Variability of thrombosis among homozygous siblings with resistance to activated protein C due to an Arg-to-Gln mutation in the gene for factor V
    Greengard, JS; Eichinger, S; Griffin, JH; Bauer, KA
  • To determine the frequency of Factor V Leiden in cases of Deep Vein Thrombosis and Healthy controls
    Saeed, A; Sumreen, M; Kashif, MA
  • Molecular analysis of factor V Leiden, factor V Hong Kong, factor II G20210A, methylenetetrahydrofolate reductase C677T, and A1298C mutations related to Turkish thrombosis patients
    Dölek, B; Eraslan, S; Eroğlu, S; Kesim, BE; Ulutin, T; Yalçıner, A; Laleli, YR; Gözükirmızı, N
  • Risk of venous thromboembolism associated with single and combined effects of Factor V Leiden, Prothrombin 20210A and Methylenetethraydrofolate reductase C677T: a meta-analysis involving over 11,000 cases and 21,000 controls
    Simone, B; Stefano, V; Leoncini, E; Zacho, J; Martinelli, I; Emmerich, J; Rossi, E; Folsom, AR; Almawi, WY; Scarabin, PY; Heijer, M
  • Prevalence of factor V Leiden, FII G20210A, FXIII Val34Leu and MTHFR C677T polymorphisms in cancer patients with and without venous thrombosis
    Ramacciotti, E; Wolosker, N; Puech-Leao, P; Zeratti, EA; Gusson, PR; Giglio, A; Franco, RF
  • Frequency of genetic mutations associated with thromboembolism in the Western Black Sea Region
    Tug, E; Aydin, H; Kaplan, E; Dogruer, D
  • Hyperhomocysteinemia and the compound heterozygous state for methylene tetrahydrofolate reductase are independent risk factors for deep vein thrombosis among South Indians
    Naushad, SM; Jamal, MN; Angalena, R; Prasad, CK; Devi, AR
  • MTHFR C677T and factor V Leiden in recurrent pregnancy loss: a study among an endogamous group in North India
    Mukhopadhyay, R; Saraswathy, KN; Ghosh, PK