Prevalence and genetic diversity of bovine kobuvirus in China

Prevalence and genetic diversity of bovine kobuvirus in China A total of 166 faecal specimens from diarrheic cattle were collected in China for detection of bovine kobuvirus (BKV) by reverse transcription PCR (RT-PCR) targeting the region a portion of the 3D nonstructural protein, with an amplicon size of 631 bp. The RNA corresponding to the BKV 3D region was detected in 34.9 % of faecal samples (58/166) in four major dairy-cattle-production areas in China, and sequence analysis based on the partial 3D sequences (35/58) indicated that the Chinese BKVs shared 88.9-96.2 % nucleotide sequence identity to BKV reference strains. Further phylogenetic analysis based on the complete VP1-encoding sequences (17/35) revealed that the Chinese BKVs shared 81-83.4 % nucleotide sequence identity to the U-1 strain, and these Chinese BKV strains, together with the U-1 strain, are apparently divided into four lineages, representing four genotypes of BKV, designated as A, B, C and D. Our results show that BKV infection is widely distributed, with high genetic diversity in China. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Archives of Virology Springer Journals

Prevalence and genetic diversity of bovine kobuvirus in China

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Publisher
Springer Vienna
Copyright
Copyright © 2014 by Springer-Verlag Wien
Subject
Biomedicine; Virology; Medical Microbiology; Infectious Diseases
ISSN
0304-8608
eISSN
1432-8798
D.O.I.
10.1007/s00705-013-1961-7
Publisher site
See Article on Publisher Site

References

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    Carmona-Vicente, N; Buesa, J; Brown, PA; Merga, JY; Darby, AC; Stavisky, J; Sadler, L; Gaskell, RM; Dawson, S; Radford, AD
  • Molecular detection of bovine kobuviruses in Italy
    Martino, B; Profio, F; Felice, E; Ceci, C; Pistilli, MG; Marsilio, F
  • Canine kobuviruses in diarrhoeic dogs in Italy
    Martino, B; Felice, E; Ceci, C; Profio, F; Marsilio, F
  • Three clusters of bovine kobuvirus isolated in Korea, 2008–2010
    Jeoung, HY; Lim, JA; Jeong, W; Oem, JK; An, DJ
  • Molecular detection of kobuvirus sequences in stool samples collected from healthy pigs in Japan
    Khamrin, P; Maneekarn, N; Hidaka, S; Kishikawa, S; Ushijima, K; Okitsu, S; Ushijima, H

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