Presence of Adrenergic Receptors in Rat Endolymphatic Sac Epithelial Cells

Presence of Adrenergic Receptors in Rat Endolymphatic Sac Epithelial Cells Intravenous application of catecholamines produces a depression in the endolymphatic sac direct current potential (ESP) and increases endolymphatic pressure via the β-adrenergic receptor (AR) in guinea pigs, suggesting that catecholamines play a role in the endolymphatic system. However, the localization of ARs in the endolymphatic sac (ES) is still undetermined. The presence of ARs in the rat ES was investigated by reverse transcriptase-polymerase chain reaction using laser capture microdissection (LCM) and immunohistochemical analysis. Expression of α1A-, α1B-, α2A-, α2B-, β1-, β2- and β3-ARs was observed in LCM samples of ES epithelia. Immunohistochemical analysis using specific antibodies showed immunofluorescence of β2- and β3-ARs in epithelial cells of the ES intermediate portion, and no specific staining results were obtained for α1-, α2A-, α2B- and β1-ARs. The presence of β2-AR with no clear immunostaining of β1-AR in ES epithelial cells is in accordance with previous electrophysiological and pharmacological results, which suggests that β2-AR mediates the action of catecholamines on the ESP. The presence of β3-AR in the ES epithelial cells and its absence in the stria vascularis implies that β3-AR plays a specific role in the ES. The Journal of Membrane Biology Springer Journals

Presence of Adrenergic Receptors in Rat Endolymphatic Sac Epithelial Cells

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Copyright © 2012 by Springer Science+Business Media New York
Life Sciences; Biochemistry, general; Human Physiology
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