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Preparation of cellulose nanofibrils for imaging purposes: comparison of liquid cryogens for rapid vitrification

Preparation of cellulose nanofibrils for imaging purposes: comparison of liquid cryogens for... Artifact-free imaging of cellulose nanofibrils (CNFs) from aqueous nanocellulose suspensions is nontrivial due to frequent irreversible agglomeration and structure damage during the course of sample preparation, especially as water is solidified prior to freeze-drying. In this study, we have examined the morphologies of CNF suspensions prepared by rapid vitrification in two different liquid cryogens—nitrogen and ethane—followed by freeze-drying. Results obtained by scanning electron microscopy confirm that vitrification in liquid ethane not only greatly improves the survivability of fine-scale CNF structural elements but also significantly reduces the propensity for CNF to agglomerate. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Cellulose Springer Journals

Preparation of cellulose nanofibrils for imaging purposes: comparison of liquid cryogens for rapid vitrification

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Publisher
Springer Journals
Copyright
Copyright © 2018 by Springer Science+Business Media B.V., part of Springer Nature
Subject
Chemistry; Bioorganic Chemistry; Physical Chemistry; Organic Chemistry; Polymer Sciences; Ceramics, Glass, Composites, Natural Materials; Sustainable Development
ISSN
0969-0239
eISSN
1572-882X
DOI
10.1007/s10570-018-1854-8
Publisher site
See Article on Publisher Site

Abstract

Artifact-free imaging of cellulose nanofibrils (CNFs) from aqueous nanocellulose suspensions is nontrivial due to frequent irreversible agglomeration and structure damage during the course of sample preparation, especially as water is solidified prior to freeze-drying. In this study, we have examined the morphologies of CNF suspensions prepared by rapid vitrification in two different liquid cryogens—nitrogen and ethane—followed by freeze-drying. Results obtained by scanning electron microscopy confirm that vitrification in liquid ethane not only greatly improves the survivability of fine-scale CNF structural elements but also significantly reduces the propensity for CNF to agglomerate.

Journal

CelluloseSpringer Journals

Published: Jun 5, 2018

References