ISSN 1070-4272, Russian Journal of Applied Chemistry, 2016, Vol. 89, No. 2, pp. 293−296. © Pleiades Publishing, Ltd., 2016.
Preparation and Characterization of Temperature Response
Molecularly Imprinted Membrane
with Chitosan and Methylmethacrylate
, Xu Zhice
, Cao Xi
, Qi Lian
, and Xuefang Zheng
School of Chemical and Pharmaceutical Engineering, Hebei University of Science and Technology,
Shi Jiazhuang 050018, P.R. China
Institute of Science and Technology Development, Hebei University of Science and Technology,
Shi Jiazhuang City 050018, P. R. China
Technical Center of Great Wall Motor Company Limited, Great Wall Motor Company Limited,
Bao Ding 071000, P.R. China
College of Chemical Engineering, Hebei Normal University of Science and Technology, Qinhuangdao 066600, P.R. China
Received February 10, 2016
Abstract—In this study, a novel temperature controlled molecularly imprinted membrane (Temp-Ctrl-MIM) made
of chitosan and methyl methacrylate was fabricated with bovine serum albumin (BSA) as template molecule. The
ﬁ lm was characterized by infrared spectroscopy (IR), X-ray diffraction (XRD), scanning electron microscopy
(SEM) and the permeation experiment. The results revealed that the porous structure of the ﬁ lm was as similar
as the BSA. Moreover, the membrane had advanced molecular imprinting capability to BSA comparing to pepsin
and casein at any temperature and the ﬁ lm demonstrated an excellent ability to identify speciﬁ c template molecule
(BSA) at the synthesis temperature comparing to other temperatures.
The molecular imprinting has exceptional potential
for synthesizing artiﬁ cial molecular recognition materials
with speciﬁ c binding cavities capable of recognizing
target molecules [1–3]. Molecularly imprinted membrane
(MIM) technology is a new kind of membrane separation
technology of molecular imprinting effect with advantage
of continuous operation, low energy consumption [4, 5].
Despite its plenty of merits, there is a signiﬁ cant drawback
that the process of molecular imprinting is negative to
controlling the process of template molecule recognition
and there is no such literature to report it.
In this paper, we demonstrate a novel Temp-Ctrl-MIM
which was compounded by chitosan and methyl meth-
acrylate (MMA) with BSA as template molecule. Our
approach to fabricate the thin ﬁ lm was shown in Fig. 1.
Chitosan is a natural polysaccharide obtained by the
partial deacetylation of chitin and it displays an excellent
molecular imprinting ability [6, 7]. MMA is applied in
synthesis of thermo-responsive shape memory polymers
which have the capability of changing their shapes from
a temporary shape to a permanent shape upon applied
external thermal stimulus . In order to compound the
Temp-Ctrl-MIM, MMA was grafted onto chitosan and
BSA was added into the reaction system as template
molecule at certain temperature which can stimulate the
Temp-Ctrl-MIM to release the template molecule. The
effects of temperature controlling with the products were
investigated by the instrument showed in Fig. 2a.
Materials: chitosan (deacetylation degree 85% and
molecular weight 5.7 kDa), ammonium persulfate, methyl
methacrylate (MMA), bovine serum albumin (BSA)
The text was submitted by the authors in English.