ISSN 10674136, Russian Journal of Ecology, 2010, Vol. 41, No. 2, pp. 189–191. © Pleiades Publishing, Ltd., 2010.
Original Russian Text © E.A. Sils, 2010, published in Ekologiya, 2010, No. 2, pp. 158–160.
In recent decades, the marsh frog (
in Russia has been expanding beyond its natural range.
Fisheries, as well as medical and biological institutions
using this species in experiments, are the sources of
accidental introduction. The northeastern expansion
of the marsh frog has become possible due to industrial
activities and the resultant ubiquitous thermal anoma
lies of anthropogenic origin (the Middle Urals,
Tomsk, Yakutsk, and Altai) (Belimov and Sedalish
chev, 1980; Kuranova, 2003; Toporkova, 1978; Yakov
The marsh frog is one of the most resistant to envi
ronmental pollution among amphibian species.
It inhabits not only waters polluted with household
wastes or fertilizers, but also the vicinities of large met
allurgic and chemical enterprises, where other
amphibians cannot live. Bodies of water located close
to the sources of pollution can be used by adult frogs
but are absolutely unsuitable for the development of
eggs and tadpoles (Misyura, 1989).
Blood as an internal medium of the body is known
to be sensitive to harmful environmental factors;
therefore, various hematological parameters can be
used for estimating the physiological state of animals.
Amphibians, especially representatives of the genus
, have welldeveloped hematopoietic and immune
systems (Manning and Horton, 1982). In addition,
amphibians experience especially long and intense
exposure to harmful environmental factors because of
their specific ontogeny.
To date, the specificity of the hematological
parameters of amphibians living in anthropogenically
transformed environments has been dealt with in rela
tively few studies (Zhukova and Peskova, 1999; Isaeva
and Vyazov, 1997; Tarasenko, 1981; Chiesa et al.,
2006; etc.), mostly on adult animals. The purpose of
this study was to estimate the physiological state of a
new generation by analyzing the specificity of the
cytomorphological composition of the peripheral
blood of postmetamorphic marsh frogs in urbanized
Postmetamorphic marsh frogs were collected from
populations living in parts of the Yekaterinburg city
agglomeration differing in the degrees of urbanization
and pollution in the periods from August to late Sep
tember of the years 2000–2006. Four zones were dis
Population Characteristics of Hematological Parameters
of the Peripheral Blood of Postmetamorphic
in Urbanized Areas
E. A. Sils
Institute of Plant and Animal Ecology, Ural Division, Russian Academy of Sciences,
ul. Vos’mogo Marta 202, Yekaterinburg, 620144 Russia;
Received July 18, 2008
: marsh frog, hematological parameters, leukogram, urbanization.
Differences in leukogram parameters between postmetamorphic
from different zones
Zone of multistoried
Park forest zone
Total of neutrophils