Get 20M+ Full-Text Papers For Less Than $1.50/day. Start a 14-Day Trial for You or Your Team.

Learn More →

Poorly Differentiated Clusters Predict a Poor Prognosis for External Auditory Canal Carcinoma

Poorly Differentiated Clusters Predict a Poor Prognosis for External Auditory Canal Carcinoma Squamous cell carcinoma (SCC) of the external auditory canal (EAC) is rare and offers a poor prognosis; more accurate prognostic biomarkers are required. Our laboratory recently demonstrated that tumor budding, characterized by tumor cell clusters (< 5 cells), and laminin 5-γ2 staining of SCC of the EAC are associated with shorter survival. However, clusters composed of ≥ 5 tumor cells are also found in the stroma. Previous reports of colorectal cancer suggest that poorly differentiated clusters (PDCs) are a negative prognostic indicator. Here, we report on the association between PDCs and prognosis in SCC of the EAC. PDCs and tumor budding were histopathologically and immunohistochemically (cytokeratin AE1/AE3) analyzed in 31 cases of pre-treatment biopsy SCC of the EAC. Clusters in the stroma composed of < or ≥ 5 cancer cells were defined as tumor budding or PDCs, respectively. Entire tumors were initially scanned to identify greatest PDC density. Tumors with low or high PDC density were classified as low- and high-grade, respectively. Patients with high-grade PDCs had a significantly poorer outcome than those with low-grade. Even in cases of low-grade tumor budding, those with high-grade PDCs had a poor prognosis. Multivariate analysis results indicated that high-grade PDCs were associated with poor prognosis. PDC grade can provide a more accurate prognosis than tumor budding in SCC of the EAC. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Head and Neck Pathology Springer Journals

Poorly Differentiated Clusters Predict a Poor Prognosis for External Auditory Canal Carcinoma

Download PDF
10 pages

Loading next page...
 
/lp/springer_journal/poorly-differentiated-clusters-predict-a-poor-prognosis-for-external-D98t4OM7Wn
Publisher
Springer Journals
Copyright
Copyright © 2018 by Springer Science+Business Media, LLC, part of Springer Nature
Subject
Medicine & Public Health; Pathology; Otorhinolaryngology; Oral and Maxillofacial Surgery; Dentistry
eISSN
1936-0568
DOI
10.1007/s12105-018-0939-x
Publisher site
See Article on Publisher Site

Abstract

Squamous cell carcinoma (SCC) of the external auditory canal (EAC) is rare and offers a poor prognosis; more accurate prognostic biomarkers are required. Our laboratory recently demonstrated that tumor budding, characterized by tumor cell clusters (< 5 cells), and laminin 5-γ2 staining of SCC of the EAC are associated with shorter survival. However, clusters composed of ≥ 5 tumor cells are also found in the stroma. Previous reports of colorectal cancer suggest that poorly differentiated clusters (PDCs) are a negative prognostic indicator. Here, we report on the association between PDCs and prognosis in SCC of the EAC. PDCs and tumor budding were histopathologically and immunohistochemically (cytokeratin AE1/AE3) analyzed in 31 cases of pre-treatment biopsy SCC of the EAC. Clusters in the stroma composed of < or ≥ 5 cancer cells were defined as tumor budding or PDCs, respectively. Entire tumors were initially scanned to identify greatest PDC density. Tumors with low or high PDC density were classified as low- and high-grade, respectively. Patients with high-grade PDCs had a significantly poorer outcome than those with low-grade. Even in cases of low-grade tumor budding, those with high-grade PDCs had a poor prognosis. Multivariate analysis results indicated that high-grade PDCs were associated with poor prognosis. PDC grade can provide a more accurate prognosis than tumor budding in SCC of the EAC.

Journal

Head and Neck Pathology – Springer Journals

Published: May 30, 2018

References

  • Anatomy and orientation of human external ear
    Alvord, LS; Farmer, BL
  • The ear: its anatomy, physiology, and diseases
    Burnett, CH
  • Epidermal migration in the ear: the location and characteristics of the generati center revealed by utilizing a radioactive desoxyribose nucleic acid precursor
    Litton, WB
  • Epithelial migration on the tymapanic membrane and external canal
    Makino, K; Amatsu, M
  • Tumors of the ear and temporal bone
    Jackler, RK; Driscoll, CLW
  • Epidemiology of cancer of the middle ear cleft
    Morton, RP; Stell, PM; Derrick, PP
  • The role of facial palsy in staging squamous cell carcinoma of the temporal bone and external auditory canal: a comparative survival analysis
    Higgins, TS; Antonio, SA
  • State of the art in temporal bone malignancies
    Prasad, SC; D’Orazio, F; Medina, M
  • Squamous cell carcinoma of the external auditory canal: an evaluation of a staging system
    Moody, SA; Hirsch, BE; Myers, EN
  • Expression of the tumour-suppressor maspin in temporal bone carcinoma
    Marioni, G; Zanoletti, E; Stritoni, P
  • Review of the literature and a recommended system of malignancy grading in oral squamous cell carcinomas
    Anneroth, G; Batsakis, J; Luna, M
  • The invasive front of carcinomas. The most important area for tumour prognosis?
    Bryne, M; Boysen, M; Alfsen, CG
  • Tumor budding in head and neck squamous cell carcinoma-a systematic review
    Almangush, A; Salo, T; Hangstrom, J
  • New criteria for histologic grading of colorectal cancer
    Ueno, H; Kajiwara, Y; Shimazaki, H
  • Prognostic value of poorly differentiated clusters in invasive breast cancer
    Sun, Y; Liang, F; Cao, W
  • Tumor budding and laminin 5-gamma 2 in squamous cell carcinoma of the external auditory canal are associated with shorter survival
    Okado, Y; Aoki, M; Hamasaki, M
  • Squamous cell carcinoma of the external auditory canal and middle ear: an operation combined with preoperative chemoradiotherapy and a free surgical margin
    Nakagawa, T; Kumamoto, Y; Natori, Y
  • General rules for clinical studies on head and neck cancer
  • Tumor budding in colorectal carcinoma assessed by cytokeratin immunostaining and budding areas: possible involvement of c-Met
    Satoh, K; Nimura, S; Aoki, M
  • Japanese classification of colorectal carcinoma
  • Classifying collective cancer cell invasion
    Friedl, P; Locker, J; Sahai, E
  • Collective cell migration in morphogenesis, regeneration and cancer
    Friedl, P; Gilmour, D
  • Prespecification and plasticity: shifting mechanisms of cell migration
    Friedl, P
  • Organizing moving groups during morphogenesis
    Lecaudey, V; Gilmour, D
  • Collective guidance of collective cell migration
    Rorth, P
  • Mechanisms of collective cell migration at a glance
    Ilina, O; Friedl, P
  • Collective cell movement in primary melanoma explants: plasticity of cell-cell interaction, beta1-integrin function, and migration strategies
    Hegerfeldt, Y; Tusch, M; Brocker, EB
  • Mammary collective cell migration involves transient loss of epithelial features and individual cell migration within the epithelium
    Ewald, AJ; Huebner, RJ; Palsdottir, H
  • Multiple rows of cells behind an epithelial wound edge extend cryptic lamellipodia to collectively drive cell-sheet movement
    Farooqui, R; Fenteany, G
  • Migration of coordinated cell clusters in mesenchymal and epithelial cancer explants in vitro
    Friedl, P; Noble, PB; Walton, PA
  • Molecular mechanisms of cancer cell invasion and plasticity
    Wolf, K; Friedl, P
  • Tubular invasion and the morphogenesis of tumor budding in colorectal carcinoma
    Prall, F; Ostwald, T; Linnebacher, M
  • Squamous cell epithelioma of the lip
    Broders, AC
  • Carcinoma of the mouth: types and degrees of malignancy
    Broders, AC
  • The microscopic grading of cancer
    Broders, AC
  • Histologic classification and grading of malignancy in carcinoma of the larynx
    Jakobsson, PA; Eneroth, CM; Killander, D
  • Malignancy grading of the deep invasive margins of oral squamous cell carcinomas has high prognostic value
    Bryne, M; Koppang, HS; Lilleng, R
  • New malignancy grading is a better prognostic indicator than Broders’ grading in oral squamous cell carcinomas
    Bryne, M; Koppang, HS; Lilleng, R
  • A retrospective analysis of histological prognostic factors for the development of lymph node metastases from auricular squamous cell carcinoma
    Clark, RR; Soutar, DS; Hunter, KD
  • Tumour budding activity and cell nest size determine patient outcome in oral squamous carcinoma: proposal for an adjusted grading system
    Boxberg, M; Jesinghaus, M; Dorfner, C
  • Inherent grading characteristics of individual pathologists contribute to clinically and prognostically relevant interobserver discordance concerning Broaders’ grading of penile squamous cell carcinomas
    Gunia, S; Burger, M; Hakenberg, OW
  • Invasive squamous cell carcinoma of the uterine cervix. II. Reproducibility of a histopathologic malignancy grading system
    Stendahl, U; Willen, H; Willen, R
  • Reproducibility of two malignancy grading systems with reportedly prognostic value for oral cancer patients
    Bryne, M; Nielsen, K; Koppang, HS
  • Tumor budding as prognostic factor in stage I/II colorectal carcinoma
    Prall, F; Nizze, H; Barten, M
  • Histologic grading based on counting poorly differentiated clusters in preoperative biopsy predicts nodal involvement and pTNM stage in colorectal cancer patients
    Barresi, V; Bonetti, LR; Ieni, A