Polymorphism of Thermostable Proteins in Soft Wheat Seedlings during Low-Temperature Acclimation

Polymorphism of Thermostable Proteins in Soft Wheat Seedlings during Low-Temperature Acclimation The accumulation of thermostable stress proteins during hardening was studied in etiolated seedlings of spring (cvs. Rollo, Drott, Angara-86, and Tyumenskaya-80) and winter (moderately frost-resistant cv. Bezostaya-1 and highly frost-resistant cvs. Irkutskaya ozimaya and Zalarinka) wheat using one-dimensional SDS-PAGE. Hardening was performed at 4°C for nine days. Seedling tolerance to low subzero temperatures was estimated from electrolyte leakage and seedling survival after freezing. Hardening of all wheat genotypes tested resulted in the accumulation of thermostable cold-regulated (COR) polypeptides with mol wts of 209, 196, 169, 66, 50, and 41 kD. A densitometric analysis demonstrated a close correlation between the cultivar frost tolerance and the relative content of COR proteins, which evidently indicated the protective functions of the latter. These results led us to suggest that the level of specific protective agents, thermostable high-molecular-weight COR polypeptides in particular, determines the degree of plant frost resistance within a particular plant species. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Russian Journal of Plant Physiology Springer Journals

Polymorphism of Thermostable Proteins in Soft Wheat Seedlings during Low-Temperature Acclimation

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Publisher
Kluwer Academic Publishers-Plenum Publishers
Copyright
Copyright © 2001 by MAIK “Nauka/Interperiodica”
Subject
Life Sciences; Plant Sciences
ISSN
1021-4437
eISSN
1608-3407
D.O.I.
10.1023/A:1012568810905
Publisher site
See Article on Publisher Site

Abstract

The accumulation of thermostable stress proteins during hardening was studied in etiolated seedlings of spring (cvs. Rollo, Drott, Angara-86, and Tyumenskaya-80) and winter (moderately frost-resistant cv. Bezostaya-1 and highly frost-resistant cvs. Irkutskaya ozimaya and Zalarinka) wheat using one-dimensional SDS-PAGE. Hardening was performed at 4°C for nine days. Seedling tolerance to low subzero temperatures was estimated from electrolyte leakage and seedling survival after freezing. Hardening of all wheat genotypes tested resulted in the accumulation of thermostable cold-regulated (COR) polypeptides with mol wts of 209, 196, 169, 66, 50, and 41 kD. A densitometric analysis demonstrated a close correlation between the cultivar frost tolerance and the relative content of COR proteins, which evidently indicated the protective functions of the latter. These results led us to suggest that the level of specific protective agents, thermostable high-molecular-weight COR polypeptides in particular, determines the degree of plant frost resistance within a particular plant species.

Journal

Russian Journal of Plant PhysiologySpringer Journals

Published: Oct 10, 2004

References

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