The prolactin gene (PRL) is a potential candidate gene for the goat cashmere traits in markerassisted selection. Thus, the aim of this study was to detect PRL gene polymorphism and its association with fiber traits in 200 Raini cashmere goats native to the south-east of Iran. A 196-bp fragment encoding exon 5 within the goat PRL gene was amplified using PCR specific primers. The amplification products were subjected to the single stranded conformation polymorphism (SSCP) analysis. Three different SSCP banding patterns (CC, AC, and AA) were observed in exon 5 of the caprine PRL gene. The pattern frequencies for CC, AC, and AA were 0.39, 0.38, and 0.23 and frequencies of the A and C alleles were 0.42 and 0.58, respectively. The genotypic distributions did not deviate from the Hardy–Weinberg equilibrium (P > 0.05). The number of observed alleles, number of effective alleles, expected heterozygosity, observed heterozygosity, mean of heterozygosity, expected homozygosity, observed homozygosity, Nei’s index and Shanon’s index were 2.0, 1.9, 0.48, 0.38, 0.48, 0.51, 0.61, 0.48, and 0.68, respectively. Results of association between genotypes and fiber traits indicated that the CC genotype had the highest fiber length compared with the AA and AC genotypes (P > 0.05) while there was no significant association between the PRL gene genotypes and fiber diameter. These results imply that the PRL gene polymorphism can be used as a molecular marker to improve fiber production without a negative effect on fiber diameter.
Russian Journal of Genetics – Springer Journals
Published: May 19, 2016
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