Polymorphism of the prolactin gene and its effect on fiber traits in goat

Polymorphism of the prolactin gene and its effect on fiber traits in goat The prolactin gene (PRL) is a potential candidate gene for the goat cashmere traits in markerassisted selection. Thus, the aim of this study was to detect PRL gene polymorphism and its association with fiber traits in 200 Raini cashmere goats native to the south-east of Iran. A 196-bp fragment encoding exon 5 within the goat PRL gene was amplified using PCR specific primers. The amplification products were subjected to the single stranded conformation polymorphism (SSCP) analysis. Three different SSCP banding patterns (CC, AC, and AA) were observed in exon 5 of the caprine PRL gene. The pattern frequencies for CC, AC, and AA were 0.39, 0.38, and 0.23 and frequencies of the A and C alleles were 0.42 and 0.58, respectively. The genotypic distributions did not deviate from the Hardy–Weinberg equilibrium (P > 0.05). The number of observed alleles, number of effective alleles, expected heterozygosity, observed heterozygosity, mean of heterozygosity, expected homozygosity, observed homozygosity, Nei’s index and Shanon’s index were 2.0, 1.9, 0.48, 0.38, 0.48, 0.51, 0.61, 0.48, and 0.68, respectively. Results of association between genotypes and fiber traits indicated that the CC genotype had the highest fiber length compared with the AA and AC genotypes (P > 0.05) while there was no significant association between the PRL gene genotypes and fiber diameter. These results imply that the PRL gene polymorphism can be used as a molecular marker to improve fiber production without a negative effect on fiber diameter. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Russian Journal of Genetics Springer Journals

Polymorphism of the prolactin gene and its effect on fiber traits in goat

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Publisher
Pleiades Publishing
Copyright
Copyright © 2016 by Pleiades Publishing, Inc.
Subject
Biomedicine; Human Genetics; Animal Genetics and Genomics; Microbial Genetics and Genomics
ISSN
1022-7954
eISSN
1608-3369
D.O.I.
10.1134/S1022795416040098
Publisher site
See Article on Publisher Site

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