Polymorphism of the cox1 gene in cercariae isolates of bird schistosomes (Trematoda:Schistosomatidae) from ponds of Moscow and Moscow region

Polymorphism of the cox1 gene in cercariae isolates of bird schistosomes... Polymorphism of a 810-bp fragment of mitochondrial cox1 gene was studied in 15 cercariae isolates of bird schistosomes (family Schistosomatidae), which were collected in water bodies of Moscow and Moscow oblast and represented three species: Trichobilharzia szidati, T. franki, and T. regenti. A substantial predominance of AT (65.4%) was characteristic of the cox1 sequences in all three species. Rare single nucleotide substitutions determined low (0.2–0.9%) intraspecific nucleotide and amino acid sequence diversity. Haplotype diversity h was high (80–100%) in all three species, suggesting a unique character for almost all cox1 sequences in the sample. Phylogenetic trees based on the nucleotide and amino acid sequence variations were constructed to study the relationships of the three schistosome species. A high support was observed for the main branching node that reflects differentiation of the monophyletic group Trichobilharzia and species of the genera Bilharziella (B. polonica), Dendritobilharzia (D. pulverulenta), and Gigantobilharzia (G. huronensis). Based on the nucleotide substitutions and amino acid polymorphisms, two groups of isolates, which parasitize Lymnaea stagnalis (T. szidati) and snails of the group Radix (T. franki and T. regenti) respectively, were isolated in the genus Trichobilharzia. The time of divergence between the two schistosome groups infecting snails of the genera Radix and Lymnaea was calculated from the cox1 nucleotide substitution rate, which is known for Asian and Indian blood flukes from the genus Schistosoma and is 2–3% per million years on average. Divergence of the three bird schistosome species under study and divergence of the Asian species of mammalian schistosomes were almost concurrent, dating back to 2.5–3.8 Myr ago. Factors responsible for the lack of intraspecific subdivision with respect to the cox1 in bird schistosomes and the lack of separation between two species (T. franki and T. regenti) are discussed. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Russian Journal of Genetics Springer Journals

Polymorphism of the cox1 gene in cercariae isolates of bird schistosomes (Trematoda:Schistosomatidae) from ponds of Moscow and Moscow region

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Publisher
SP MAIK Nauka/Interperiodica
Copyright
Copyright © 2010 by Pleiades Publishing, Ltd.
Subject
Biomedicine; Microbial Genetics and Genomics; Animal Genetics and Genomics; Human Genetics
ISSN
1022-7954
eISSN
1608-3369
D.O.I.
10.1134/S1022795410070136
Publisher site
See Article on Publisher Site

Abstract

Polymorphism of a 810-bp fragment of mitochondrial cox1 gene was studied in 15 cercariae isolates of bird schistosomes (family Schistosomatidae), which were collected in water bodies of Moscow and Moscow oblast and represented three species: Trichobilharzia szidati, T. franki, and T. regenti. A substantial predominance of AT (65.4%) was characteristic of the cox1 sequences in all three species. Rare single nucleotide substitutions determined low (0.2–0.9%) intraspecific nucleotide and amino acid sequence diversity. Haplotype diversity h was high (80–100%) in all three species, suggesting a unique character for almost all cox1 sequences in the sample. Phylogenetic trees based on the nucleotide and amino acid sequence variations were constructed to study the relationships of the three schistosome species. A high support was observed for the main branching node that reflects differentiation of the monophyletic group Trichobilharzia and species of the genera Bilharziella (B. polonica), Dendritobilharzia (D. pulverulenta), and Gigantobilharzia (G. huronensis). Based on the nucleotide substitutions and amino acid polymorphisms, two groups of isolates, which parasitize Lymnaea stagnalis (T. szidati) and snails of the group Radix (T. franki and T. regenti) respectively, were isolated in the genus Trichobilharzia. The time of divergence between the two schistosome groups infecting snails of the genera Radix and Lymnaea was calculated from the cox1 nucleotide substitution rate, which is known for Asian and Indian blood flukes from the genus Schistosoma and is 2–3% per million years on average. Divergence of the three bird schistosome species under study and divergence of the Asian species of mammalian schistosomes were almost concurrent, dating back to 2.5–3.8 Myr ago. Factors responsible for the lack of intraspecific subdivision with respect to the cox1 in bird schistosomes and the lack of separation between two species (T. franki and T. regenti) are discussed.

Journal

Russian Journal of GeneticsSpringer Journals

Published: Jul 22, 2010

References

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