Polymorphism of PCR profiles and expression of alleles at the locus Adh1 in agamospermous progeny of sugar beet Beta vulgaris L.

Polymorphism of PCR profiles and expression of alleles at the locus Adh1 in agamospermous progeny... A modification of the ISSR amplification method based on using a combination of microsatellite and specific unique primer is proposed and tested. This modification simplifies the detected PCR profiles and allows the examination of DNA regions containing definite genes. Combinations of microsatellite primer Mic2 (5′-gacag-acaga-cagac-a-3′) and one of the primers specific to the Adh1 locus, which controls alcohol dehydrogenase (ADH1) in sugar beet, were employed in this work. The microsatellite primer was used in combination with the following specific primers: Adh1f (5′-agagt-gttgg-agagg-gtgtg-ac-3′) containing the binding site at the fourth exon of gene Adh1, or Adh1r (5′-act(ct)a-cagca-ag(ct)cc-(ct)ac(ct)g-ctcc-3′) that binds to the fifth exon of the same gene. In the agamospermous progeny of individual heterozygous diploid plants of sugar beet with the Adh1-F/Adh1-S genotype, polymorphism of PCR profiles obtained in plants of each of three phenotypic classes (FF, FS, and SS) was detected. Among plants of the progeny from an individual plant that represents the heterozygous phenotypic class FS, differences were revealed not only between the PCR profiles but also in the relative activity of allele isozymes of ADH1. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Russian Journal of Genetics Springer Journals

Polymorphism of PCR profiles and expression of alleles at the locus Adh1 in agamospermous progeny of sugar beet Beta vulgaris L.

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Publisher
SP MAIK Nauka/Interperiodica
Copyright
Copyright © 2008 by MAIK Nauka
Subject
Biomedicine; Microbial Genetics and Genomics; Animal Genetics and Genomics; Human Genetics
ISSN
1022-7954
eISSN
1608-3369
D.O.I.
10.1134/S1022795408090123
Publisher site
See Article on Publisher Site

Abstract

A modification of the ISSR amplification method based on using a combination of microsatellite and specific unique primer is proposed and tested. This modification simplifies the detected PCR profiles and allows the examination of DNA regions containing definite genes. Combinations of microsatellite primer Mic2 (5′-gacag-acaga-cagac-a-3′) and one of the primers specific to the Adh1 locus, which controls alcohol dehydrogenase (ADH1) in sugar beet, were employed in this work. The microsatellite primer was used in combination with the following specific primers: Adh1f (5′-agagt-gttgg-agagg-gtgtg-ac-3′) containing the binding site at the fourth exon of gene Adh1, or Adh1r (5′-act(ct)a-cagca-ag(ct)cc-(ct)ac(ct)g-ctcc-3′) that binds to the fifth exon of the same gene. In the agamospermous progeny of individual heterozygous diploid plants of sugar beet with the Adh1-F/Adh1-S genotype, polymorphism of PCR profiles obtained in plants of each of three phenotypic classes (FF, FS, and SS) was detected. Among plants of the progeny from an individual plant that represents the heterozygous phenotypic class FS, differences were revealed not only between the PCR profiles but also in the relative activity of allele isozymes of ADH1.

Journal

Russian Journal of GeneticsSpringer Journals

Published: Sep 25, 2008

References

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