Pof8 is a La-related protein and a constitutive
component of telomerase in ﬁssion yeast
Diego J. Páez-Moscoso
, Lili Pan
, Rutendo F. Sigauke
, Morgan R. Schroeder
, Wen Tang
& Peter Baumann
Telomerase reverse transcriptase (TERT) and the non-coding telomerase RNA subunit (TR)
constitute the core of telomerase. Here we now report that the putative F-box protein Pof8 is
also a constitutive component of active telomerase in ﬁssion yeast. Pof8 functions in a
hierarchical assembly pathway by promoting the binding of the Lsm2-8 complex to telo-
merase RNA, which in turn promotes binding of the catalytic subunit. Loss of Pof8 reduces
TER1 stability, causes a severe assembly defect, and results in critically short telomeres.
Structure proﬁle searches identiﬁed similarities between Pof8 and telomerase subunits from
ciliated protozoa, making Pof8 next to TERT the most widely conserved telomerase subunits
identiﬁed to date.
Stowers Institute for Medical Research, Kansas City, MO 64110, USA.
Howard Hughes Medical Institute, Kansas City, MO 64110, USA.
Molecular and Integrative Physiology, University of Kansas Medical Center, Kansas City, MO 66160, USA.
Institute of Developmental Biology and
Neurobiology, Johannes Gutenberg University, 55099 Mainz, Germany.
Present address: RNA Therapeutics Institute, University of Massachusetts Medical
School, Worcester, MA 01605, USA. Correspondence and requests for materials should be addressed to P.B. (email: firstname.lastname@example.org)