Plasmolysis as a Tool to Reveal Lead Localization in the Apoplast of Root Cells

Plasmolysis as a Tool to Reveal Lead Localization in the Apoplast of Root Cells In order to analyze the distribution of lead between cell walls and plasmalemma, two-day-old maize seedlings (Zea mays L.) were incubated for 24 h on a solution of lead nitrate at a concentration causing 50% inhibition of root growth (10–5 M). Using the histochemical technique (precipitation of lead dithizonate), the distribution of lead in plasmolyzed and nonplasmolyzed cells of the root cortex was compared. This allowed us to separate the lead bound by cell walls from the lead located on the protoplast surface and in the periplasmic space. The plasmolysis was conducted prior to histochemical reaction by the incubation of seedling roots in 0.6 M sucrose solution for 30 min. The lead precipitates were located in cell walls and on the surface of protoplast. A small amount of lead was found in periplasmic space of some cells in root cortex. It is suggested that the lead is bound not only to the cell wall matrix but also to the plasmalemma. Russian Journal of Plant Physiology Springer Journals

Plasmolysis as a Tool to Reveal Lead Localization in the Apoplast of Root Cells

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Kluwer Academic Publishers-Plenum Publishers
Copyright © 2002 by MAIK “Nauka/Interperiodica”
Life Sciences; Plant Sciences
Publisher site
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