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R. Morris, A. Tousson, D. Benos, J. Schafer (1998)
Microtubule disruption inhibits AVT-stimulated Cl- secretion but not Na+ reabsorption in A6 cells.American journal of physiology. Renal physiology, 274 2
H. Hansma, Jan Hoh (1994)
Biomolecular imaging with the atomic force microscope.Annual review of biophysics and biomolecular structure, 23
M. Beckmann, P. Nollert, H. Kolb (1998)
Manipulation and Molecular Resolution of a Phosphatidylcholine-Supported Planar Bilayer by Atomic Force MicroscopyThe Journal of Membrane Biology, 161
Stefan Schneider, Marie Egan, B. Jena, W. Guggino, Hans Oberleithner, John Geibel (1999)
Continuous detection of extracellular ATP on living cells by using atomic force microscopy.Proceedings of the National Academy of Sciences of the United States of America, 96 21
S. Cunningham, R. Worrell, D. Benos, R. Frizzell (1992)
cAMP-stimulated ion currents in Xenopus oocytes expressing CFTR cRNA.The American journal of physiology, 262 3 Pt 1
(1996)
Wild type but not delta F508 CFTR inhibits Na
R. Morris, A. Tousson, D. Benos, J. Schafer (1998)
Microtubule disruption inhibits AVT-stimulated Cl- secretion but not Na+ reabsorption in A6 cells.The American journal of physiology, 274 2
S. Schneider, J. Lärmer, R. Henderson, H. Oberleithner (1998)
Molecular weights of individual proteins correlate with molecular volumes measured by atomic force microscopyPflügers Archiv, 435
G. Lukács, G. Segal, N. Kartner, S. Grinstein, F. Zhang (1997)
Constitutive internalization of cystic fibrosis transmembrane conductance regulator occurs via clathrin-dependent endocytosis and is regulated by protein phosphorylation.The Biochemical journal, 328 ( Pt 2)
Lawrence Prince, A. Tousson, R. Marchase (1993)
Cell surface labeling of CFTR in T84 cells.The American journal of physiology, 264 2 Pt 1
R. Henderson, H. Oberleithner (2000)
Pushing, pulling, dragging, and vibrating renal epithelia by using atomic force microscopy.American journal of physiology. Renal physiology, 278 5
K. Peters, J. Qi, Simon Watkins, R. Frizzell (2000)
Mechanisms underlying regulated CFTR trafficking.The Medical clinics of North America, 84 3
B. Moyer, J. Loffing, E. Schwiebert, D. Loffing‐Cueni, P. Halpin, K. Karlson, Iskandar Ismailov, W. Guggino, G. Langford, B. Stanton (1998)
Membrane Trafficking of the Cystic Fibrosis Gene Product, Cystic Fibrosis Transmembrane Conductance Regulator, Tagged with Green Fluorescent Protein in Madin-Darby Canine Kidney Cells*The Journal of Biological Chemistry, 273
A. Tousson, C. Fuller, D. Benos (1996)
Apical recruitment of CFTR in T-84 cells is dependent on cAMP and microtubules but not Ca2+ or microfilaments.Journal of cell science, 109 ( Pt 6)
C. Grimellec, E. Lesniewska, C. Cachia, J. Schreiber, F. Fornel, J. Goudonnet (1994)
Imaging of the membrane surface of MDCK cells by atomic force microscopy.Biophysical journal, 67 1
M. Madeja, U. Muβhoff, D. Kuhlmann, E. Speckmann (1991)
Membrane currents elicited by the epileptogenic drug pentylenetetrazole in the native oocyte ofXenopus laevisBrain Research, 553
J. Tabcharani, X. Chang, J. Riordan, J. Hanrahan (1991)
Phosphorylation-regulated CI− channel in CHO cells stably expressing the cystic fibrosis geneNature, 352
M. Radmacher, RW Tillamnn, M. Fritz, H. Gaub (1992)
From molecules to cells: imaging soft samples with the atomic force microscope.Science, 257 5078
B. Zerhusen, J. Zhao, J. Xie, P. Davis, J. Ma (1999)
A Single Conductance Pore for Chloride Ions Formed by Two Cystic Fibrosis Transmembrane Conductance Regulator Molecules*The Journal of Biological Chemistry, 274
J. Hoh, P. Hansma (1992)
Atomic force microscopy for high-resolution imaging in cell biology.Trends in cell biology, 2 7
Seng Cheng, D. Rich, J. Marshall, R. Gregory, M. Welsh, Alan Smith (1991)
Phosphorylation of the R domain by cAMP-dependent protein kinase regulates the CFTR chloride channelCell, 66
G. Denning, L. Ostedgaard, Seng Cheng, Alan Smith, M. Welsh (1992)
Localization of cystic fibrosis transmembrane conductance regulator in chloride secretory epithelia.The Journal of clinical investigation, 89 1
M. Hug, I. Thiele, R. Greger (1997)
The role of exocytosis in the activation of the chloride conductance in Chinese hamster ovary cells (CHO) stably expressing CFTRPflügers Archiv, 434
Jie Yang, L. Tamm, Andrew Somlyo, Z. Shao (1993)
Promises and problems of biological atomic force microscopyJournal of Microscopy, 171
S. Seino (1999)
ATP-sensitive potassium channels: a model of heteromultimeric potassium channel/receptor assemblies.Annual review of physiology, 61
M. Mall, A. Hipper, R. Greger, K. Kunzelmann (1996)
Wild type but not ΔF508 CFTR inhibits Na+ conductance when coexpressed in Xenopus oocytesFEBS Letters, 381
H. Schillers, T. Danker, H. Schnittler, F. Lang, H. Oberleithner (2000)
Plasma Membrane Plasticity of Xenopus laevis Oocyte Imaged with Atomic Force MicroscopyCellular Physiology and Biochemistry, 10
T. Takahashi, K. Matsushita, M. Welsh, J. Stokes (1994)
Effect of cAMP on intracellular and extracellular ATP content of Cl- -secreting epithelia and 3T3 fibroblasts.The Journal of biological chemistry, 269 27
J. Lärmer, S. Schneider, T. Danker, A. Schwab, H. Oberleithner (1997)
Imaging excised apical plasma membrane patches of MDCK cells in physiological conditions with atomic force microscopyPflügers Archiv, 434
W. Weber, H. Cuppens, J. Cassiman, W. Clauss, W. Driessche (1999)
Capacitance measurements reveal different pathways for the activation of CFTRPflügers Archiv, 438
Lía Pietrasanta, Douglas Thrower, Wan Hsieh, Shashirekha Rao, Olaf Stemmann, Johannes Lechner, John Carbon, Helen Hansma (1999)
Probing the Saccharomyces cerevisiae centromeric DNA (CEN DNA)-binding factor 3 (CBF3) kinetochore complex by using atomic force microscopy.Proceedings of the National Academy of Sciences of the United States of America, 96 7
R. Lehrich, S. Aller, P. Webster, C. Marino, J. Forrest (1998)
Vasoactive intestinal peptide, forskolin, and genistein increase apical CFTR trafficking in the rectal gland of the spiny dogfish, Squalus acanthias. Acute regulation of CFTR trafficking in an intact epithelium.The Journal of clinical investigation, 101 4
L. Prince, R. Workman, R. Marchase (1994)
Rapid endocytosis of the cystic fibrosis transmembrane conductance regulator chloride channel.Proceedings of the National Academy of Sciences of the United States of America, 91 11
M. Howard, M. Duvall, D. Devor, J. Dong, K. Henze, R. Frizzell (1995)
Epitope tagging permits cell surface detection of functional CFTR.The American journal of physiology, 269 6 Pt 1
Q. Al-Awqati (1995)
Regulation of ion channels by ABC transporters that secrete ATPScience, 269
E. Schwiebert, F. Gesek, L. Ercolani, C. Wjasow, D. Gruenert, K. Karlson, Bruce Stanton (1994)
Heterotrimeric G proteins, vesicle trafficking, and CFTR Cl- channels.The American journal of physiology, 267 1 Pt 1
Membrane trafficking of the cystic fibrosis transmembrane conductance regulator (CFTR) is supposed to be an important mechanism controlled by the intracellular messenger cAMP. This has been shown with fluorescence techniques, electron microscopy and membrane capacitance measurements. In order to visualize protein insertion we applied atomic force microscopy (AFM) to inside-out oriented plasma membrane patches of CFTR-expressing Xenopus laevis oocytes before and after cAMP-stimulation. In a first step, oocytes injected with CFTR-cRNA were voltage-clamped, verifying successful CFTR expression. Water-injected oocytes served as controls. Then, plasma membrane patches were excised, placed (inside out) on glass and scanned by AFM. Before cAMP-stimulation plasma membranes of both water-injected and CFTR-expressing oocytes contained about 200 proteins per μm2. Molecular protein masses were estimated from molecular volumes measured by AFM. Before cAMP-stimulation, protein distribution showed a peak value of 11 nm protein height corresponding to 475 kDa. During cAMP-stimulation with 1 mm isobutylmethylxanthine (IBMX) plasma membrane protein density increased in water-injected oocytes to 700 proteins per μm2 while the peak value shifted to 7 nm protein height corresponding to 95 kDa. In contrast, CFTR-expressing oocytes showed after cAMP-stimulation about 400 proteins per μm2 while protein distribution exhibited two peak values, one peak at 10 nm protein height corresponding to 275 kDa and another one at 14 nm corresponding to 750 kDa. They could represent heteromeric protein clusters associated with CFTR. In conclusion, we visualized plasma membrane protein insertion upon cAMP-stimulation and quantified protein distribution with AFM at molecular level. We propose that CFTR causes clustering of plasma membrane proteins.
The Journal of Membrane Biology – Springer Journals
Published: Mar 19, 2014
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