Access the full text.
Sign up today, get DeepDyve free for 14 days.
M. Kononov, Burgund Bassuner, S. Gelvin (1997)
Integration of T-DNA binary vector 'backbone' sequences into the tobacco genome: evidence for multiple complex patterns of integration.The Plant journal : for cell and molecular biology, 11 5
A. Kusnadi, Z. Nikolov, J. Howard (1997)
Production of recombinant proteins in transgenic plants: Practical considerationsBiotechnology and Bioengineering, 56
D. Garrick, S. Fiering, David Martin, E. Whitelaw (1998)
Repeat-induced gene silencing in mammalsNature Genetics, 18
S. Buck, C. Wilde, M. Montagu, A. Depicker (2000)
T-DNA vector backbone sequences are frequently integrated into the genome of transgenic plants obtained by Agrobacterium-mediated transformationMolecular Breeding, 6
Jin-Hao Liu, L. Selinger, K. Cheng, K. Beauchemin, Maurice Moloney (1997)
Plant seed oil-bodies as an immobilization matrix for a recombinant xylanase from the rumen fungus Neocallimastix patriciarumMolecular Breeding, 3
D. Witcher, E. Hood, D. Peterson, Michele Bailey, D. Bond, A. Kusnadi, R. Evangelista, Z. Nikolov, C. Wooge, R. Mehigh, W. Kappel, J. Register, J. Howard (1998)
Commercial production of β-glucuronidase (GUS): a model system for the production of proteins in plantsMolecular Breeding, 4
C. Napoli, C. Lemieux, R. Jorgensen (1990)
Introduction of a Chimeric Chalcone Synthase Gene into Petunia Results in Reversible Co-Suppression of Homologous Genes in trans.The Plant cell, 2
J. Ma, B. Hikmat, K. Wycoff, N. Vine, D. Chargelegue, Lloyd Yu, M. Hein, T. Lehner (1998)
Characterization of a recombinant plant monoclonal secretory antibody and preventive immunotherapy in humansNature Medicine, 4
H. Vaucheret, C. Béclin, T. Elmayan, F. Feuerbach, C. Godon, J.-B. Morel, P. Mourrain, J.-C. Palauqui, S. Vernhettes (1998)
Transgene-induced gene silencing in plantsPlant J., 16
N. Magnuson, P. Linzmaier, R. Reeves, G. An, K. HayGlass, James Lee (1998)
Secretion of biologically active human interleukin-2 and interleukin-4 from genetically modified tobacco cells in suspension culture.Protein expression and purification, 13 1
C. Tacket, H. Mason, G. Losonsky, J. Clements, M. Levine, C. Arntzen (1998)
Immunogenicity in humans of a recombinant bacterial antigen delivered in a transgenic potatoNature Medicine, 4
L. Luzzatto, M. Bessler, B. Rotoli (1997)
Somatic Mutations in Paroxysmal Nocturnal Hemoglobinuria: A Blessing in Disguise?Cell, 88
R. Holmes-Davis, L. Comai (1998)
Nuclear matrix attachment regions and plant gene expressionTrends in Plant Science, 3
J. Yoder, C. Walsh, T. Bestor (1997)
Cytosine methylation and the ecology of intragenomic parasites.Trends in genetics : TIG, 13 8
M. Metzlaff, Michael O'dell, P. Cluster, Richard Flavell (1997)
RNA-Mediated RNA Degradation and Chalcone Synthase A Silencing in PetuniaCell, 88
A. Thompson, S. Myatt (1997)
Tetracycline-dependent activation of an upstream promoter reveals transcriptional interference between tandem genes within T-DNA in tomatoPlant Molecular Biology, 34
H. Vaucheret (1993)
Identification of a general silencer for 19S and 35S promoters in a transgenic tobacco plant : 90 bp of homology in the promoter sequence are sufficient for trans-inactivation, 316
P. Breyne, M. Montagu, A. Depicker, G. Gheysen (1992)
Characterization of a plant scaffold attachment region in a DNA fragment that normalizes transgene expression in tobacco.The Plant cell, 4 4
(1998)
Direct gene transfer: chemical, electrical and physical methds
V. Ramanathan, K. Veluthambi (1995)
Transfer of non-T-DNA portions of the Agrobacterium tumefaciens Ti plasmid pTiA6 from the left terminus of TL-DNAPlant Molecular Biology, 28
M. Neve, M. Loose, A. Jacobs, H. Houdt, B. Kaluza, U. Weidle, M. Montagu, A. Depicker (1993)
Assembly of an antibody and its derived antibody fragment inNicotiana andArabidopsisTransgenic Research, 2
Maike Tam, J. Oseph, N. Mol, M. Ooter, Biocentrum Amsterdam (1997)
THE SILENCE OF GENES IN TRANSGENIC PLANTSAnnals of Botany, 79
P. Cluster, M. O'dell, M. Metzlaff, R. Flavell (1996)
Details of T-DNA structural organization from a transgenic Petunia population exhibiting co-suppressionPlant Molecular Biology, 32
J. Jakowitsch, I. Papp, E. Moscone, J. Winden, M. Matzke, A. Matzke (1999)
Molecular and cytogenetic characterization of a transgene locus that induces silencing and methylation of homologous promoters in trans.The Plant journal : for cell and molecular biology, 17 2
A. Wenck, M. Czakó, Ivan Kanevski, L. Marton (1997)
Frequent collinear long transfer of DNA inclusive of the whole binary vector during Agrobacterium-mediated transformationPlant Molecular Biology, 34
Kazuko Morino, O. Olsen, K. Shimamoto (1999)
Silencing of an aleurone-specific gene in transgenic rice is caused by a rearranged transgene.The Plant journal : for cell and molecular biology, 17 3
E. Graaff, A. Dulk-Ras, P. Hooykaas (1996)
Deviating T-DNA transfer fromAgrobacterium tumefaciens to plantsPlant Molecular Biology, 31
O. Goddijn, J. Pen (1995)
Plants as bioreactorsTrends in Biotechnology, 13
R. Jorgensen, Christine Snyder, Jonathan Jones (1987)
T-DNA is organized predominantly in inverted repeat structures in plants transformed with Agrobacterium tumefaciens C58 derivativesMolecular and General Genetics MGG, 207
J. Kooter, M. Matzke, P. Meyer (1999)
Listening to the silent genes: transgene silencing, gene regulation and pathogen control.Trends in plant science, 4 9
M. De Neve, H. Van Houdt, A.-M. Bruyns, M. Van Montagu, A. Depicker (1998)
Recombinant Proteins from Plants: Production and Isolation of Clinically Useful Compounds (Methods in Biotechnology, Vol. 3)
E. Selker (1999)
Gene Silencing Repeats that CountCell, 97
C. Hart, B. Fischer, J. Neuhaus, F. Meins (1992)
Regulated inactivation of homologous gene expression in transgenic Nicotiana sylvestris plants containing a defense-related tobacco chitinase geneMolecular and General Genetics MGG, 235
A. Bown (1999)
Virus resistance and gene silencing: killing the messenger
P. Elomaa, Y. Helariutta, M. Kotilainen, T. Teeri, R. Griesbach, P. Seppänen (1995)
Transgene inactivation inPetunia hybrida is influenced by the properties of the foreign geneMolecular and General Genetics MGG, 248
M. Neve, S. Buck, C. Wilde, H. Houdt, I. Strobbe, A. Jacobs, M. Montagu, A. Depicker (1999)
Gene silencing results in instability of antibody production in transgenic plantsMolecular and General Genetics MGG, 260
B. Phillipson (1993)
Expression of a hybrid (1–3, 1–4)-β-glucanase in barley protoplastsPlant Science, 91
J. Brandle, S. Mchugh, L. James, H. Labbé, B. Miki (1995)
Instability of Transgene Expression in Field Grown Tobacco Carrying the csr1-1 Gene for Sulfonylurea Herbicide ResistanceBio/Technology, 13
A. Wolffe (1997)
Transcription control: Repressed repeats express themselvesCurrent Biology, 7
A. Matzke, Franz Neuhuber, Y-D Park, Peter Ambros, M. Matzke (1994)
Homology-dependent gene silencing in transgenic plants: epistatic silencing loci contain multiple copies of methylated transgenesMolecular and General Genetics MGG, 244
T. Sijen, J. Wellink, J. Hiriart, A. Kammen (1996)
RNA-Mediated Virus Resistance: Role of Repeated Transgenes and Delineation of Targeted Regions.The Plant cell, 8
David Smyth (1999)
Gene silencing: Plants and viruses fight it outCurrent Biology, 9
Julio Salinas, Giorgio Matassi, Luis Montero, Giorgio Bernardi (1988)
Compositional compartmentalization and compositional patterns in the nuclear genomes of plants.Nucleic acids research, 16 10
G. Zhong, D. Peterson, D. Delaney, Michele Bailey, D. Witcher, J. III, D. Bond, Chun-Ping Li, Lisa Marshall, Ellen Kulisek, David Ritland, T. Meyer, E. Hood, J. Howard (1999)
Commercial production of aprotinin in transgenic maize seedsMolecular Breeding, 5
S. Hobbs, T. Warkentin, Catherine DeLong (2004)
Transgene copy number can be positively or negatively associated with transgene expressionPlant Molecular Biology, 21
Vibha Srivastava, Olin Anderson, David Ow (1999)
Single-copy transgenic wheat generated through the resolution of complex integration patterns.Proceedings of the National Academy of Sciences of the United States of America, 96 20
E. Hood, D. Witcher, S. Maddock, T. Meyer, C. Baszczynski, Michele Bailey, P. Flynn, J. Register, Lisa Marshall, D. Bond, Ellen Kulisek, A. Kusnadi, R. Evangelista, Z. Nikolov, C. Wooge, R. Mehigh, R. Hernan, W. Kappel, David Ritland, Chun Li, J. Howard (1997)
Commercial production of avidin from transgenic maize: characterization of transformant, production, processing, extraction and purificationMolecular Breeding, 3
T. Verwoerd, P. Paridon, A. Ooyen, J.W.M. Lent, A. Hoekema, Jan Pen (1995)
Stable Accumulation of Aspergillus niger Phytase in Transgenic Tobacco Leaves, 109
T. Arakawa, D. Chong, W. Langridge (1998)
Efficacy of a food plant-based oral cholera toxin B subunit vaccineNature Biotechnology, 16
Andreas Voss, M. Niersbach, R. Hain, H. Hirsch, Y. Liao, F. Kreuzaler, R. Fischer (1995)
Reduced virus infectivity inN. tabacum secreting a TMV-specific full-size antibodyMolecular Breeding, 1
V. Iglesias, E. Moscone, I. Papp, F. Neuhuber, S. Michalowski, T. Phelan, S. Spiker, M. Matzke, A. Matzke (1997)
Molecular and cytogenetic analyses of stably and unstably expressed transgene loci in tobacco.The Plant cell, 9 8
D. Parmenter, J. Boothe, G. Rooijen, Edward Yeung, Maurice Moloney (1995)
Production of biologically active hirudin in plant seeds using oleosin partitioningPlant Molecular Biology, 29
P. Meyer, F. Linn, I. Heidmann, H. Meyer, I. Niedenhof, H. Saedler (1992)
Endogenous and environmental factors influence 35S promoter methylation of a maize A1 gene construct in transgenic petunia and its colour phenotypeMolecular and General Genetics MGG, 231
Henrik Albert, Emily Dale, Elsa Lee, David Ow (1995)
Site-specific integration of DNA into wild-type and mutant lox sites placed in the plant genome.The Plant journal : for cell and molecular biology, 7 4
J. Pen, T. Verwoerd, P. Paridon, R. Beudeker, P. Elzen, K. Geerse, J. Klis, H. Versteegh, A. Ooyen, A. Hoekema (1993)
Phytase-containing Transgenic Seeds as a Novel Feed Additive for Improved Phosphorus UtilizationBio/Technology, 11
R. Jorgensen, P. Cluster, J. English, Q. Que, C. Napoli (1996)
Chalcone synthase cosuppression phenotypes in petunia flowers: comparison of sense vs. antisense constructs and single-copy vs. complex T-DNA sequencesPlant Molecular Biology, 31
K. Lindsey (1998)
Transgenic Plant Research
J. Palauqui, T. Elmayan, F. Borne, P. Crété, C. Charles, H. Vaucheret (1996)
Frequencies, Timing, and Spatial Patterns of Co-Suppression of Nitrate Reductase and Nitrite Reductase in Transgenic Tobacco Plants, 112
James English, Elisabeth Mueller, David Baulcombe (1996)
Suppression of Virus Accumulation in Transgenic Plants Exhibiting Silencing of Nuclear Genes.The Plant cell, 8
J. Finnegan, D. McElroy (1994)
Transgene Inactivation: Plants Fight Back!Bio/Technology, 12
F. Engelen, A. Schouten, J. Molthoff, J. Roosien, J. Salinas, W. Dirkse, A. Schots, J. Bakker, F. Gommers, M. Jongsma, D. Bosch, W. Stiekema (1994)
Coordinate expression of antibody subunit genes yields high levels of functional antibodies in roots of transgenic tobaccoPlant Molecular Biology, 26
Qiudeng Que, Huai-Yu Wang, James English, Richard Jorgensen (1997)
The Frequency and Degree of Cosuppression by Sense Chalcone Synthase Transgenes Are Dependent on Transgene Promoter Strength and Are Reduced by Premature Nonsense Codons in the Transgene Coding Sequence.The Plant cell, 9
L. Fecker, A. Kaufmann, U. Commandeur, J. Commandeur, R. Koenig, W. Burgermeister (1996)
Expression of single-chain antibody fragments (scFv) specific for beet necrotic yellow vein virus coat protein or 25 kDa protein in Escherichia coli and Nicotiana benthamianaPlant Molecular Biology, 32
M. Stam, J. Mol, J. Kooter (1997)
Review Article: The Silence of Genes in Transgenic PlantsAnnals of Botany, 79
A. Depicker, I. Ingelbrecht, H. Houdt, M. Loose, M. Montagu (1996)
Posttranscriptional reporter transgene silencing in transgenic tobacco
A. Goossens, W. Dillen, J. Clercq, M. Montagu, G. Angenon (1999)
The arcelin-5 gene of Phaseolus vulgaris directs high seed-specific expression in transgenic Phaseolus acutifolius and Arabidopsis plants.Plant physiology, 120 4
A. Hiatt, Robert Caffferkey, K. Bowdish (1989)
Production of antibodies in transgenic plantsNature, 342
A. Depicker, I. Ingelbrecht, H. Van Houdt, M. De Loose, M. Van Montagu (1996)
Mechanisms and Applications of Gene Silencing
J. Palauqui, H. Vaucheret (1995)
Field trial analysis of nitrate reductase co-suppression: a comparative study of 38 combinations of transgene lociPlant Molecular Biology, 29
M. Neve, S. Buck, A. Jacobs, M. Montagu, A. Depicker (1997)
T-DNA integration patterns in co-transformed plant cells suggest that T-DNA repeats originate from co-integration of separate T-DNAs.The Plant journal : for cell and molecular biology, 11 1
T. Elmayan, S. Balzergue, Fabrice Béon, V. Bourdon, Jérémy Daubremet, Yvette Guénet, P. Mourrain, J. Palauqui, S. Vernhettes, Tiphaine Vialle, K. Wostrikoff, H. Vaucheret (1998)
Arabidopsis Mutants Impaired in CosuppressionPlant Cell, 10
P. Meyer, I. Heidmann (1994)
Epigenetic variants of a transgenic petunia line show hypermethylation in transgene DNA: an indication for specific recognition of foreign DNA in transgenic plantsMolecular and General Genetics MGG, 243
M. Neve, H. Houdt, Anne-Marie Bruyns, M. Montagu, A. Depicker (1998)
Screening for Transgenic Lines with Stable and Suitable Accumulation Levels of a Heterologous Protein, 3
A. Depicker, M. Van Montagu (1997)
Post-transcriptional gene silencing in plantsCurr. Opin. Cell Biol., 9
K. Aspegren, L. Mannonen, A. Ritala, R. Puupponen-Pimiä, U. Kurtén, M. Salmenkallio-Marttila, V. Kauppinen, T. Teeri (2005)
Secretion of a heat-stable fungal β-glucanase from transgenic, suspension-cultured barley cellsMolecular Breeding, 2
F. de Carvalho, G. Gheysen, S. Kushnir, M. Van Montagu, D. Inzé, C. Castresana (1992)
Suppression of ?-1,3–glucanase 356 transgene expression in homozygous plantsEMBO J., 11
I. Furner, M. Sheikh, Clare Collett (1998)
Gene silencing and homology-dependent gene silencing in Arabidopsis: genetic modifiers and DNA methylation.Genetics, 149 2
R. Jorgensen (1994)
Developmental significance of epigenetic impositions on the plant genome: A paragenetic function for chromosomesDevelopmental Genetics, 15
R. Jorgensen (1995)
Cosuppression, Flower Color Patterns, and Metastable Gene Expression StatesScience, 268
J. Vandekerckhove, J. Damme, M. Lijsebettens, J. Botterman, M. Block, M. Vandewiele, A. Clercq, J. Leemans, M. Montagu, E. Krebbers (1989)
Enkephalins Prouduced in Transgenic Plants Using Modified 2S Seed Storage ProteinsBio/Technology, 7
O. Edelbaum, D. Stein, N. Holland, Y. Gafni, O. Livneh, D. Novick, M. Rubinstein, I. Sela (1992)
Expression of Active Human Interferon-β in Transgenic PlantsJournal of interferon research, 12
C. Grevelding, Verena Fantes, E. Kemper, J. Schell, R. Masterson (1993)
Single-copy T-DNA insertions in Arabidopsis are the predominant form of integration in root-derived transgenics, whereas multiple insertions are found in leaf discsPlant Molecular Biology, 23
J. Ma, A. Hiatt, M. Hein, N. Vine, Fei Wang, P. Stabila, C. Dolleweerd, K. Mostov, T. Lehner (1995)
Generation and assembly of secretory antibodies in plantsScience, 268
Nigel Kilby, H. Leyser, I. Furner (1992)
Promoter methylation and progressive transgene inactivation inArabidopsisPlant Molecular Biology, 20
C. Dehio, J. Schell (1994)
Identification of plant genetic loci involved in a posttranscriptional mechanism for meiotically reversible transgene silencing.Proceedings of the National Academy of Sciences of the United States of America, 91 12
B. Hanson, D. Engler, Y. Moy, B. Newman, E. Ralston, N. Gutterson (1999)
A simple method to enrich an Agrobacterium-transformed population for plants containing only T-DNA sequences.The Plant journal : for cell and molecular biology, 19 6
S. Gelvin (1998)
The introduction and expression of transgenes in plantsCurrent opinion in biotechnology, 9 2
O. Voinnet, D. Baulcombe (1997)
Systemic signalling in gene silencingNature, 389
H. Houdt, M. Montagu, A. Depicker (2000)
Both sense and antisense RNAs are targets for the sense transgene-induced posttranscriptional silencing mechanismMolecular and General Genetics MGG, 263
M. Lohuis, H. Galliano, I. Heidmann, P. Meyer (1995)
Treatment with propionic and butyric acid enhances expression variegation and promoter methylation in plant transgenes.Biological chemistry Hoppe-Seyler, 376 5
A. Krol, Marcel Beld, Joseph Moi, A. Stuitje (1990)
Flavonoid genes in petunia: addition of a limited number of gene copies may lead to a suppression of gene expression.The Plant cell, 2 4
D. Hemming (1995)
Molecular farming: using transgenic plants to produce novel proteins and other chemicals., 7
M. Stam, Robertus Bruin, S. Kenter, R. Hoorn, R. Blokland, J. Mol, J. Kooter (1997)
Post-transcriptional silencing of chalcone synthase in petunia by inverted transgene repeatsPlant Journal, 12
L. Mlynárová, L. Keizer, W. Stiekema, J. Nap (1996)
Approaching the Lower Limits of Transgene Variability.The Plant cell, 8
S. Matsumoto, K. Ikura, M. Ueda, R. Sasaki (1995)
Characterization of a human glycoprotein (erythropoietin) produced in cultured tobacco cellsPlant Molecular Biology, 27
K. Itoh (1996)
Homology-dependent Gene Silencing in Plants., 34
C. Peach, J. Velten (1991)
Transgene expression variability (position effect) of CAT and GUS reporter genes driven by linked divergent T-DNA promotersPlant Molecular Biology, 17
G. Gheysen, G. Angenon, M. Montagu (1998)
Agrobacterium-mediated plant transformation: a scientifically intriguing story with significant applications
J. Palauqui, T. Elmayan, J. Pollien, H. Vaucheret (1997)
Systemic acquired silencing: transgene‐specific post‐transcriptional silencing is transmitted by grafting from silenced stocks to non‐silenced scionsThe EMBO Journal, 16
A. Moffat (1995)
Exploring transgenic plants as a new vaccine sourceScience, 268
C. Koncz, K. Németh, G.P. Rédei, J. Schell (1994)
Homologous Recombination and Gene Silencing in Plants
Elizabeth Hood, J. Jilka (1999)
Plant-based production of xenogenic proteins.Current opinion in biotechnology, 10 4
M. Wassenegger, S. Heimes, Leonhard Riedel, H. Sänger (1994)
RNA-directed de novo methylation of genomic sequences in plantsCell, 76
S. Buck, A. Jacobs, M. Montagu, A. Depicker (1999)
The DNA sequences of T-DNA junctions suggest that complex T-DNA loci are formed by a recombination process resembling T-DNA integration.The Plant journal : for cell and molecular biology, 20 3
H. Houdt, I. Ingelbrecht, M. Montagu, A. Depicker (1997)
Post-transcriptional silencing of a neomycin phosphotransferase II transgene correlates with the accumulation of unproductive RNAs and with increased cytosine methylation of 3' flanking regionsPlant Journal, 12
C. Walter, I. Broer, D. Hillemann, A. Pühler (1992)
High frequency, heat treatment-induced inactivation of the phosphinothricin resistance gene in transgenic single cell suspension cultures of Medicago sativaMolecular and General Genetics MGG, 235
M. Moloney, L. Holbrook (1997)
Subcellular targeting and purification of recombinant proteins in plant production systems.Biotechnology & genetic engineering reviews, 14
H. Vaucheret, C. Beclin, M. Fagard (2001)
Post-transcriptional gene silencing in plants.Journal of cell science, 114 Pt 17
A. Wolters, L. Trindade, E. Jacobsen, R. Visser (1998)
Fluorescencein situhybridization on extended DNA fibres as a tool to analyse complex T‐DNA loci in potatoPlant Journal, 13
O. Scheid, K. Afsar, J. Paszkowski (1998)
Release of epigenetic gene silencing by trans-acting mutations in Arabidopsis.Proceedings of the National Academy of Sciences of the United States of America, 95 2
U. Fiedler, O. Artsaenko, J. Phillips, U. Conrad (1999)
Recombinant Antibodies: Applications in Plant Science and Plant Pathology
Habib Khoudi, Serge Laberge, J. Ferullo, Renee Bazin, André Darveau, Y. Castonguay, G. Allard, Réal Lemieux, Louis-P. Vézina (1999)
Production of a diagnostic monoclonal antibody in perennial alfalfa plants.Biotechnology and bioengineering, 64 2
L. Zeitlin, S. Olmsted, T. Moench, M. Co, B. Martinell, V. Paradkar, D. Russell, C. Queen, R. Cone, K. Whaley (1998)
A humanized monoclonal antibody produced in transgenic plants for immunoprotection of the vagina against genital herpesNature Biotechnology, 16
Hugh Mason, C. Arntzen (1995)
Transgenic plants as vaccine production systems.Trends in biotechnology, 13 9
S. Deroles, R. Gardner (1988)
Analysis of the T-DNA structure in a large number of transgenic petunias generated by Agrobacterium-mediated transformationPlant Molecular Biology, 11
F. Carvalho, G. Gheysen, S. Kushnir, M. Montagu, D. Inzé, anc'Carmen Castresana (1992)
Suppression of beta‐1,3‐glucanase transgene expression in homozygous plants.The EMBO Journal, 11
D. Bosch, J. Smal, E. Krebbers (1994)
A trout growth hormone is expressed, correctly folded and partially glycosylated in the leaves but not the seeds of transgenic plantsTransgenic Research, 3
J. Pen, L. Molendijk, W. Quax, P. Sijmons, A. Ooyen, P. Elzen, K. Rietveld, A. Hoekema (1992)
Production of Active Bacillus licheniformis Alpha-Amylase in Tobacco and its Application in Starch LiquefactionBio/Technology, 10
P. Meyer, I. Heidmann, I. Niedenhof (1993)
Differences in DNA-methylation are associated with a paramutation phenomenon in transgenic petunia.The Plant journal : for cell and molecular biology, 4 1
M. Matzke, M. Primig, J. Trnovsky, A. Matzke (1989)
Reversible methylation and inactivation of marker genes in sequentially transformed tobacco plantsThe EMBO Journal, 8
A. Matzke, M. Matzke (1998)
Position effects and epigenetic silencing of plant transgenes.Current opinion in plant biology, 1 2
F. Dorlhac, M. Vincentz, Y. Chupeau, H. Vaucheret (1994)
Co-suppression of nitrate reductase host genes and transgenes in transgenic tobacco plantsMolecular and General Genetics MGG, 243
B. Martineau, T. Voelker, R. Sanders (1994)
On Defining T-DNA.The Plant cell, 6
M. Stam, A. Viterbo, J. Mol, J. Kooter (1998)
Position-Dependent Methylation and Transcriptional Silencing of Transgenes in Inverted T-DNA Repeats: Implications for Posttranscriptional Silencing of Homologous Host Genes in PlantsMolecular and Cellular Biology, 18
D. Gallie (1998)
Controlling gene expression in transgenics.Current opinion in plant biology, 1 2
C. Wilde, G. Jaeger, M. Neve, M. Montagu, A. Depicker (1999)
Production of antibodies in transgenic plants: a general introduction
H. Mason, Tariq Haq, J. Clements, C. Arntzen (1998)
Edible vaccine protects mice against Escherichia coli heat-labile enterotoxin (LT): potatoes expressing a synthetic LT-B gene.Vaccine, 16 13
C. Koncz, K. Németh, G. Rédei, J. Schell (1994)
Homology Recognition During T-DNA Integration into the Plant Genome
C. Arntzen (1997)
High-tech herbal medicine: Plant-based vaccinesNature Biotechnology, 15
T. Elmayan, H. Vaucheret (1996)
Expression of single copies of a strongly expressed 35S transgene can be silenced post-transcriptionallyPlant Journal, 9
M. Block, D. Debrouwer (1991)
Two T-DNA's co-transformed intoBrassica napus by a doubleAgrobacterium tumefaciens infection are mainly integrated at the same locusTheoretical and Applied Genetics, 82
K. Herbers, I. Wilke, U. Sonnewald (1995)
A Thermostable Xylanase from Clostridium thermocellum Expressed at High Levels in the Apoplast of Transgenic Tobacco Has No Detrimental Effects and Is Easily PurifiedBio/Technology, 13
Plants are particularly attractive as large-scale production systems for proteins intended for therapeutical or industrial applications: they can be grown easily and inexpensively in large quantities that can be harvested and processed with the available agronomic infrastructures. The effective use of plants as bioreactors depends on the possibility of obtaining high protein accumulation levels that are stable during the life cycle of the transgenic plant and in subsequent generations. Silencing of the introduced transgenes has frequently been observed in plants, constituting a major commercial risk and hampering the general economic exploitation of plants as protein factories. Until now, the most efficient strategy to avoid transgene silencing involves careful design of the transgene construct and thorough analysis of transformants at the molecular level. Here, we focus on different aspects of the generation of transgenic plants intended for protein production and on their influence on the stability of heterologous gene expression.
Plant Molecular Biology – Springer Journals
Published: Oct 16, 2004
Read and print from thousands of top scholarly journals.
Already have an account? Log in
Bookmark this article. You can see your Bookmarks on your DeepDyve Library.
To save an article, log in first, or sign up for a DeepDyve account if you don’t already have one.
Copy and paste the desired citation format or use the link below to download a file formatted for EndNote
Access the full text.
Sign up today, get DeepDyve free for 14 days.
All DeepDyve websites use cookies to improve your online experience. They were placed on your computer when you launched this website. You can change your cookie settings through your browser.