Plant Regeneration from Wheat Leaf Explants

Plant Regeneration from Wheat Leaf Explants The factors affecting the callus formation and regeneration capacity of leaf explants of four genotypes of the genus Triticum, viz. T. aestivum, cvs. Taezhnaya and Chinese Spring; T. durum, cv. Kollektivnaya; and T. persicum, were investigated. The process of callus formation did not depend on the explant genotype. Apical leaf segments were characterized by the lowest capacity of callus formation. In contrast, the rate of plant regeneration was correlated with the genotype and the explant developmental stage. The highest number of regenerants was obtained from a basal segment of three-day-old seedlings ofT. aestivum, cv. Taezhnaya. The yield of plants from one explant was doubled due to the use of maltose in the regeneration medium. The prospects of using leaf segments as the explants for the genetic transformation of wheat plants are discussed. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Russian Journal of Plant Physiology Springer Journals

Plant Regeneration from Wheat Leaf Explants

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Publisher
Kluwer Academic Publishers-Plenum Publishers
Copyright
Copyright © 2003 by MAIK “Nauka/Interperiodica”
Subject
Life Sciences; Plant Sciences
ISSN
1021-4437
eISSN
1608-3407
D.O.I.
10.1023/A:1023826304989
Publisher site
See Article on Publisher Site

Abstract

The factors affecting the callus formation and regeneration capacity of leaf explants of four genotypes of the genus Triticum, viz. T. aestivum, cvs. Taezhnaya and Chinese Spring; T. durum, cv. Kollektivnaya; and T. persicum, were investigated. The process of callus formation did not depend on the explant genotype. Apical leaf segments were characterized by the lowest capacity of callus formation. In contrast, the rate of plant regeneration was correlated with the genotype and the explant developmental stage. The highest number of regenerants was obtained from a basal segment of three-day-old seedlings ofT. aestivum, cv. Taezhnaya. The yield of plants from one explant was doubled due to the use of maltose in the regeneration medium. The prospects of using leaf segments as the explants for the genetic transformation of wheat plants are discussed.

Journal

Russian Journal of Plant PhysiologySpringer Journals

Published: Oct 17, 2004

References

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