Access the full text.
Sign up today, get DeepDyve free for 14 days.
Q Guan, J Wu, Y Zhang, C Jiang, R Liu, C Chai, J Zhu (2013)
A DEAD box RNA helicase is critical for pre-mRNA splicing, cold-responsive gene regulation, and cold tolerance in ArabidopsisPlant Cell, 25
KL Rossow, R Janknecht (2003)
Synergism between p68 RNA helicase and the transcriptional coactivators CBP and p300Oncogene, 22
GJ Bates, SM Nicol, BJ Wilson, AM Jacobs, JC Bourdon, J Wardrop, DJ Gregory, DP Lane, ND Perkins, FV Fuller-Pace (2005)
The DEAD box protein p68: a novel transcriptional coactivator of the p53 tumour suppressorEMBO J, 24
H Itadani, M Sugita, M Sugiura (1994)
Structure and expression of a cDNA encoding an RNA helicase-like protein in tobaccoPlant Mol Biol, 24
BJ Wilson, GJ Bates, SM Nicol, DJ Gregory, ND Perkins, FV Fuller-Pace (2004)
The p68 and p72 DEAD box RNA helicases interact with HDAC1 and repress transcription in a promoter-specific mannerBMC Mol Biol, 5
M Okanami, T Meshi, M Iwabuchi (1998)
Characterization of a DEAD box ATPase/RNA helicase protein of Arabidopsis thalianaNucleic Acids Res, 26
N Tuteja (2003)
Plant DNA helicases: the long unwinding roadJ Exp Bot, 54
M Buszczak, AC Spradling (2006)
The Drosophila P68 RNA helicase regulates transcriptional deactivation by promoting RNA release from chromatinGenes Dev, 20
A Pradhan, VS Chauhan, R Tuteja (2005)
Plasmodium falciparum DNA helicase 60 is a schizont stage specific, bipolar and dual helicase stimulated by PKC phosphorylationMol Biochem Parasitol, 144
M Tarique, M Ahmad, A Ansari, R Tuteja (2013)
Plasmodium falciparum DOZI, an RNA helicase interacts with eIF4EGene, 522
MSA Banu, KMK Huda, N Tuteja (2014)
Isolation and functional characterization of the promoter of a DEAD-box helicase Psp68 using Agrobacterium-mediated transient assayPlant Signal Behav, 9
H Endoh, K Maruyama, Y Masuhiro, Y Kobayashi, M Goto, H Tai, J Yanagisawa, D Metzger, S Hashimoto, S Kato (1999)
Purification and identification of p68 RNA helicase acting as a transcriptional coactivator specific for the activation function 1 of human estrogen receptor alphaMol Cell Biol, 19
J Mehta, R Tuteja (2011)
A novel dual Dbp5/DDX19 homologue from Plasmodium falciparum requires Q motif for activityMol Biochem Parasitol, 176
N Tuteja, R Tuteja (2004)
Unraveling DNA helicases. Motif, structure, mechanism and functionEur J Biochem, 271
C Kammel, M Thomaier, BB Sorensen, T Schubert, G Langst, M Grasser, KD Grasser (2013)
Arabidopsis DEAD-box RNA helicase UAP56 interacts with both RNA and DNA as well as with mRNA export factorsPLoS One, 8
E Gendra, A Moreno, MM Alba, M Pages (2004)
Interaction of the plant glycine-rich RNA-binding protein MA16 with a novel nucleolar DEAD box RNA helicase protein from Zea maysPlant J, 38
N Tuteja, RK Sahoo, B Garg, N Tuteja (2013)
OsSUV3 dual helicase functions in salinity stress tolerance by maintaining photosynthesis and antioxidant machinery in rice (Oryza sativa L. cv. IR64)Plant J, 76
RJ Stevenson, SJ Hamilton, DE MacCallum, PA Hall, FV Fuller-Pace (1998)
Expression of the ?dead box? RNA helicase p68 is developmentally and growth regulated and correlates with organ differentiation/maturation in the fetusJ Pathol, 184
AA Vashisht, N Tuteja (2006)
Stress responsive DEAD-box helicases: a new pathway to engineer plant stress toleranceJ Photochem Photobiol B, 84
K Arnold, L Bordoli, J Kopp, T Schwede (2006)
The SWISS-MODEL workspace: a web-based environment for protein structure homology modellingBioinformatics, 22
L Yang, C Lin, ZR Liu (2005)
Signaling to the DEAD box?regulation of DEAD-box p68 RNA helicase by protein phosphorylationsCell Signal, 17
SP Anand, SA Khan (2004)
Structure-specific DNA binding and bipolar helicase activities of PcrANucleic Acids Res, 32
A Pradhan, VS Chauhan, R Tuteja (2005)
A novel ?DEAD-box? DNA helicase from Plasmodium falciparum is homologous to p68Mol Biochem Parasitol, 140
N Tuteja, R Tuteja (2004)
Prokaryotic and eukaryotic DNA helicases. Essential molecular motor proteins for cellular machineryEur J Biochem, 271
PG Jones, M Mitta, Y Kim, W Jiang, M Inouye (1996)
Cold shock induces a major ribosomal-associated protein that unwinds double stranded RNA in Escherichia coliProc Natl Acad Sci USA, 93
M Ahmad, A Ansari, M Tarique, AT Satsangi, R Tuteja (2012)
Plasmodium falciparum UvrD helicase translocates in 3? to 5? direction, colocalizes with MLH and modulates its activity through physical interactionPLoS One, 7
F Constantinesco, P Forterre, EV Koonin, L Aravind, CA Elie (2004)
Bipolar DNA helicase gene, herA, clusters with rad50, mre11 and nurA genes in thermophilic archaeaNucleic Acids Res, 32
GW Owttrim (2006)
RNA helicases and abiotic stressNucleic Acids Res, 34
O Cordin, J Banroques, NK Tanner, P Linder (2006)
The DEAD-box protein family of RNA helicasesGene, 367
Y Huang, ZR Liu (2002)
The ATPase, RNA unwinding, and RNA binding activities of recombinant p68 RNA helicaseJ Biol Chem, 277
J Shankar, A Pradhan, R Tuteja (2008)
Isolation and characterization of Plasmodium falciparum UAP56 homolog evidence for the coupling of RNA binding and splicing activity by site-directed mutationsArch Biochem Biophys, 478
EF Pettersen, TD Goddard, CC Huang, GS Couch, DM Greenblatt, EC Meng, TE Ferrin (2004)
UCSF Chimera?a visualization system for exploratory research and analysisJ Comput Chem, 25
M Ahmad, R Tuteja (2013)
Plasmodium falciparum RuvB2 translocates in 5??3? direction, relocalizes during schizont stage and its enzymatic activities are up regulated by RuvB3 of the same complexBiochim Biophys Acta, 1834
Z Cheng, J Coller, R Parker, H Song (2005)
Crystal structure and functional analysis of DEAD-box protein Dhh1pRNA, 11
R Tuteja, A Pradhan (2006)
Unraveling the ?DEAD-box? helicases of Plasmodium falciparumGene, 376
RD Iggo, DP Lane (1989)
Nuclear protein p68 is an RNA-dependent ATPaseEMBO J, 8
P Linder, E Jankowsky (2011)
From unwinding to clamping?the DEAD box RNA helicase familyNat Rev Mol Cell Biol, 12
H Wang, X Gao, Y Huang, J Yang, ZR Liu (2009)
P68 RNA helicase is a nucleocytoplasmic shuttling proteinCell Res, 19
M Hogbom, R Collins, S Berg, RM Jenvert, T Karlberg, T Kotenyova, A Flores, GB Karlsson Hedestam, LH Schiavone (2007)
Crystal structure of conserved domains 1 and 2 of the human DEAD-box helicase DDX3X in complex with the mononucleotide AMPJ Mol Biol, 372
A Pradhan, R Tuteja (2007)
Bipolar, dual Plasmodium falciparum helicase 45 expressed in the intraerythrocytic developmental cycle is required for parasite growthJ Mol Biol, 373
T Yang, BW Poovaiah (2003)
Calcium/calmodulin-mediated signal network in plantsTrends Plant Sci, 8
FV Fuller-Pace (2006)
DExD/H box RNA helicases multifunctional proteins with important roles in transcriptional regulationNucleic Acids Res, 34
FV Fuller-Pace (2013)
The DEAD box proteins DDX5 (p68) and DDX17 (p72): multi-tasking transcriptional regulatorsBiochim Biophys Acta, 1829
H Wang, X Gao, JJ Yang, ZR Liu (2013)
Interaction between p68 RNA helicase and Ca2+?calmodulin promotes cell migration and metastasisNat Commun, 4
AA Vashisht, A Pradhan, R Tuteja, N Tuteja (2005)
Cold- and salinity stress-induced bipolar pea DNA helicase 47 is involved in protein synthesis and stimulated by phosphorylation with protein kinase CPlant J, 44
Z Gong, CH Dong, H Lee, J Zhu, L Xiong, D Gong, B Stevenson, JK Zhu (2005)
A DEAD box RNA helicase is essential for mRNA export and important for development and stress responses in ArabidopsisPlant Cell, 17
DEAD-box helicases play essential role in DNA and RNA metabolism such as replication, repair, recombination, transcription, translation, ribosome biogenesis and splicing which regulate plant growth and development. The presence of helicases in the stress-induced ORFs identified by cDNA microarray indicates that helicases might be playing an important role in stabilizing growth in plants under stress. p68 DEAD-box helicase has been identified and characterized from animal systems but the properties and functions of plant p68 are poorly understood. In this study, the identification, purification and characterization of recombinant p68 from Pisum sativum (Psp68) is presented. Psp68 possesses all the characteristic motifs like DEAD-box ATP-binding and helicase C terminal motifs and is structurally similar to human p68 homologue. Psp68 exhibits ATPase activity in the presence of both DNA and RNA and it binds to DNA as well as RNA. It contains the characteristic RNA helicase activity. Interestingly Psp68 also shows the unique DNA helicase activity, which is bipolar in nature (unwinds DNA in both the 5′–3′ and 3′–5′ directions). The Km values of Psp68 for ATPase are 0.5126 and 0.9142 mM in the presence of DNA and RNA, respectively. The Km values of Psp68 are 1.6129 and 1.14 nM for DNA helicase and RNA helicase, respectively. The unique properties of Psp68 suggest that it could be a multifunctional protein involved in different aspect of DNA and RNA metabolism. This discovery should make an important contribution to better understanding of nucleic acids metabolism plants.
Plant Molecular Biology – Springer Journals
Published: Jun 8, 2014
Read and print from thousands of top scholarly journals.
Already have an account? Log in
Bookmark this article. You can see your Bookmarks on your DeepDyve Library.
To save an article, log in first, or sign up for a DeepDyve account if you don’t already have one.
Copy and paste the desired citation format or use the link below to download a file formatted for EndNote
Access the full text.
Sign up today, get DeepDyve free for 14 days.
All DeepDyve websites use cookies to improve your online experience. They were placed on your computer when you launched this website. You can change your cookie settings through your browser.