Pineapple translation factor SUI1 and ribosomal protein L36
promoters drive constitutive transgene expression patterns
in Arabidopsis thaliana
Received: 18 October 2012 / Accepted: 12 December 2012 / Published online: 22 December 2012
Ó Springer Science+Business Media Dordrecht 2012
Abstract The availability of a variety of promoter sequen-
ces is necessary for the genetic engineering of plants, in basic
research studies and for the development of transgenic crops.
In this study, the promoter and 5
untranslated regions of the
evolutionally conserved protein translation factor SUI1 gene
and ribosomal protein L36 gene were isolated from pineap-
ple and sequenced. Each promoter was translationally fused
to the GUS reporter gene and transformed into the heterol-
ogous plant system Arabidopsis thaliana. Both the pineapple
SUI1 and L36 promoters drove GUS expression in all tissues
of Arabidopsis at levels comparable to the CaMV35S pro-
moter. Transient assays determined that the pineapple SUI1
promoter also drove GUS expression in a variety of cli-
macteric and non-climacteric fruit species. Thus the pine-
apple SUI1 and L36 promoters demonstrate the potential for
using translation factor and ribosomal protein genes as a
source of promoter sequences that can drive constitutive
transgene expression patterns.
Ribosomal protein L36 Á SUI1 Á Promoter Á Constitutive Á
GUS Á Pineapple Á Arabidopsis
Access to a variety of well characterized promoters with a
broad spectrum of expression patterns provides researchers
with important molecular tools for controlling transgene
expression in basic research and biotechnology applications.
Tissue speciﬁc promoters are useful for directing tight con-
trol of gene expression, particularly when off target
expression may have a negative effect on plant development.
Examples include the use of a tapetum speciﬁc promoter to
drive stilbene synthase transgene expression in tobacco
anthers to achieve pollen ablation (Hoﬁg et al. 2003, 2006),
and the use of the harvest inducible asparagine synthetase
promoter from asparagus to express a cysteine protease
antisense transgene and an ACC oxidase antisense trans-
gene to delay ﬂoret senescence in harvested broccoli
(Winichayakul et al. 2004; Eason et al. 2005; Gapper et al.
Conversely, constitutive promoters are used to deliver
transgene expression to most or all tissues and stages of
development. Applications where constitutive promoters
are particularly useful include the expression of selectable
marker genes, herbicide resistance genes, insect resistance
genes, over-expression and gene knockout constructs in
reverse genetic studies. Some of the most commonly used
constitutive promoters in plants originate from plant viru-
ses, with the cauliﬂower mosaic virus 35S promoter
(CaMV35S) extensively utilized to drive high levels of
transgene expression in multiple plant species (Odell et al.
1985; Fang et al. 1989; Benfey et al. 1990; Potenza et al.
2004; Datta et al. 1992). Other plant viral promoters used
to drive high transgene expression include the cassava vein
mosaic virus (CsVMV) promoter (Verdaguer et al. 1996),
Australian banana streak virus (BSV) promoters (Remans
et al. 2001), mirabilis mosaic virus (MMV) promoter (Dey
and Maiti 1999) and the ﬁgwort mosaic virus (FMV) pro-
moter (Sanger et al. 1990; Maiti et al. 1997). However,
there are limitations to the use of virally derived promoters,
including reports of post-transcriptional transgene silencing
Jonni Koia, Richard Moyle contributed equally to the work.
Electronic supplementary material The online version of this
article (doi:10.1007/s11103-012-0002-3) contains supplementary
material, which is available to authorized users.
J. Koia Á R. Moyle Á C. Hendry Á L. Lim Á J. R. Botella (&)
University of Queensland, Brisbane 4072, Australia
Plant Mol Biol (2013) 81:327–336