Physiological Characterization of the Yeast Plasma Membrane Outward Rectifying K+ Channel, DUK1 (TOK1), In Situ

Physiological Characterization of the Yeast Plasma Membrane Outward Rectifying K+ Channel, DUK1... The major voltage-dependent ion channel in the plasma membrane of Saccharomyces cerevisiae, a conspicuous outwardly rectifying K+ channel, was first dubbed YPK1 and later renamed according to its registered gene names (DUK1, TOK1). It has proven novel in both structure and function. Whole-cell patch-clamp studies of the channel directly on yeast protoplasts now extend our earlier description obtained from isolated patches of yeast membrane (Bertl & Slayman, 1992; Bertl et al., 1993), and provide new data both on the contributions of channel properties to yeast physiology and on possible contributions of molecular structure to channel properties. Three recording tactics produce completely equivalent results and thereby allow great flexibility in the design of experiments: whole-cell voltage clamp with sustained voltage steps (∼2.5 sec), whole-cell voltage clamp with slow voltage ramps (5 sec, −40 to +100 mV), and time-averaging of single-channel currents. The Journal of Membrane Biology Springer Journals

Physiological Characterization of the Yeast Plasma Membrane Outward Rectifying K+ Channel, DUK1 (TOK1), In Situ

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Copyright © Inc. by 1998 Springer-Verlag New York
Life Sciences; Biochemistry, general; Human Physiology
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