Physiological Characterization of the Yeast Plasma Membrane Outward Rectifying K+ Channel, DUK1 (TOK1), In Situ

Physiological Characterization of the Yeast Plasma Membrane Outward Rectifying K+ Channel, DUK1... The major voltage-dependent ion channel in the plasma membrane of Saccharomyces cerevisiae, a conspicuous outwardly rectifying K+ channel, was first dubbed YPK1 and later renamed according to its registered gene names (DUK1, TOK1). It has proven novel in both structure and function. Whole-cell patch-clamp studies of the channel directly on yeast protoplasts now extend our earlier description obtained from isolated patches of yeast membrane (Bertl & Slayman, 1992; Bertl et al., 1993), and provide new data both on the contributions of channel properties to yeast physiology and on possible contributions of molecular structure to channel properties. Three recording tactics produce completely equivalent results and thereby allow great flexibility in the design of experiments: whole-cell voltage clamp with sustained voltage steps (∼2.5 sec), whole-cell voltage clamp with slow voltage ramps (5 sec, −40 to +100 mV), and time-averaging of single-channel currents. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png The Journal of Membrane Biology Springer Journals

Physiological Characterization of the Yeast Plasma Membrane Outward Rectifying K+ Channel, DUK1 (TOK1), In Situ

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Publisher
Springer-Verlag
Copyright
Copyright © Inc. by 1998 Springer-Verlag New York
Subject
Life Sciences; Biochemistry, general; Human Physiology
ISSN
0022-2631
eISSN
1432-1424
D.O.I.
10.1007/s002329900343
Publisher site
See Article on Publisher Site

Abstract

The major voltage-dependent ion channel in the plasma membrane of Saccharomyces cerevisiae, a conspicuous outwardly rectifying K+ channel, was first dubbed YPK1 and later renamed according to its registered gene names (DUK1, TOK1). It has proven novel in both structure and function. Whole-cell patch-clamp studies of the channel directly on yeast protoplasts now extend our earlier description obtained from isolated patches of yeast membrane (Bertl & Slayman, 1992; Bertl et al., 1993), and provide new data both on the contributions of channel properties to yeast physiology and on possible contributions of molecular structure to channel properties. Three recording tactics produce completely equivalent results and thereby allow great flexibility in the design of experiments: whole-cell voltage clamp with sustained voltage steps (∼2.5 sec), whole-cell voltage clamp with slow voltage ramps (5 sec, −40 to +100 mV), and time-averaging of single-channel currents.

Journal

The Journal of Membrane BiologySpringer Journals

Published: Mar 1, 1998

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