Physiological and ultrastructural changes in Arabidopsis thaliana as affected by changed GSH level and Cu excess

Physiological and ultrastructural changes in Arabidopsis thaliana as affected by changed GSH... We investigated the responses of wild-type Arabidopsis thaliana plants to the excess of Cu under conditions of the changed intracellular level of reduced glutathione (GSH) after application of buthionine sulfoximine (BSO) or exogenous GSH to the nutrient solution. BSO (500 µM) decreased and exogenous GSH (500 µM) increased GSH level, while increasing Cu concentration (from 5 to 50 µM) resulted in a decreased GSH content in the roots, but in its increased content in the shoots. BSO did not affect plant growth in contrast to exogenous GSH and Cu, which significantly reduced plant fresh weight. Both Cu and BSO or GSH induced changes in the root structure and leaf chloroplasts ultrastructure. Cu did not induce phytochelatin accumulation. Application of BSO or exogenous GSH did not significantly affect either the GSH level in the Cu-treated plants (except for 50/50 and 50/500 µM/µM Cu/GSH treatments increasing intracellular GSH content in the roots) or Cu toxicity to plants. These results suggest that GSH is not directly involved in Cu detoxification and tolerance in A. thaliana, yet it influences the proper anatomical structure of plants. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Russian Journal of Plant Physiology Springer Journals

Physiological and ultrastructural changes in Arabidopsis thaliana as affected by changed GSH level and Cu excess

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Publisher
SP MAIK Nauka/Interperiodica
Copyright
Copyright © 2009 by Pleiades Publishing, Ltd.
Subject
Life Sciences; Plant Sciences ; Plant Physiology
ISSN
1021-4437
eISSN
1608-3407
D.O.I.
10.1134/S1021443709060120
Publisher site
See Article on Publisher Site

Abstract

We investigated the responses of wild-type Arabidopsis thaliana plants to the excess of Cu under conditions of the changed intracellular level of reduced glutathione (GSH) after application of buthionine sulfoximine (BSO) or exogenous GSH to the nutrient solution. BSO (500 µM) decreased and exogenous GSH (500 µM) increased GSH level, while increasing Cu concentration (from 5 to 50 µM) resulted in a decreased GSH content in the roots, but in its increased content in the shoots. BSO did not affect plant growth in contrast to exogenous GSH and Cu, which significantly reduced plant fresh weight. Both Cu and BSO or GSH induced changes in the root structure and leaf chloroplasts ultrastructure. Cu did not induce phytochelatin accumulation. Application of BSO or exogenous GSH did not significantly affect either the GSH level in the Cu-treated plants (except for 50/50 and 50/500 µM/µM Cu/GSH treatments increasing intracellular GSH content in the roots) or Cu toxicity to plants. These results suggest that GSH is not directly involved in Cu detoxification and tolerance in A. thaliana, yet it influences the proper anatomical structure of plants.

Journal

Russian Journal of Plant PhysiologySpringer Journals

Published: Nov 6, 2009

References

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