Physical methods

Physical methods Despite the advent of high-output, recombinant DNA-based screening strategies, many important protein-protein interactions in the plant cell have been and still are revealed using co-sedimentation, affinity chromatography and other affinity techniques, co-immunoprecipitation and cross-linking. The advantages of these techniques, the care that should be taken interpreting the data obtained and the possible ways to overcome pitfalls are illustrated. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Plant Molecular Biology Springer Journals

Physical methods

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Publisher
Kluwer Academic Publishers
Copyright
Copyright © 2002 by Kluwer Academic Publishers
Subject
Life Sciences; Biochemistry, general; Plant Sciences; Plant Pathology
ISSN
0167-4412
eISSN
1573-5028
D.O.I.
10.1023/A:1021209702115
Publisher site
See Article on Publisher Site

Abstract

Despite the advent of high-output, recombinant DNA-based screening strategies, many important protein-protein interactions in the plant cell have been and still are revealed using co-sedimentation, affinity chromatography and other affinity techniques, co-immunoprecipitation and cross-linking. The advantages of these techniques, the care that should be taken interpreting the data obtained and the possible ways to overcome pitfalls are illustrated.

Journal

Plant Molecular BiologySpringer Journals

Published: Oct 13, 2004

References

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