Physical and genetic mapping of the prosaposin gene (PSAP) to bovine Chromosome 28

Physical and genetic mapping of the prosaposin gene (PSAP) to bovine Chromosome 28 Mammalian Genome 8, Brief Data Reports 73 Physical and genetic mapping of the prosaposin gene (PSAP) to bovine Chromosome 28 C. Elduque, 1 L. Li, 2 W. Barendse, 3 J.E. Womack 2 1Facultad de Veterinaria, Universidad de Zaragoza, Miguel Servet 177, 50013 Zaragoza, Spain 2Department of Veterinary Pathobiology, Texas A&M University, College Station, Texas 77843, USA 3CSIRO, Division of Tropical Animal Production, Research Road, University of Queensland, St. Lucia, Brisbane, 4072, Australia Received: 3 July 1996 / Accepted: 27 August 1996 Species: Cattle Locus name: Prosaposin Fig. 1. Segregation in two cattle Locus symbol: PSAP families of PSAP polymorphisms Map position: BTA28. Linkage was detected to the anonymous after digestion with HindlIl (A) and DNA polymorphism D28S4 (theta 0.22, Iodscore 4.50). PstI (B) and probed with a human Method of mapping: Southern analysis of somatic cell hybrids [1] PSAP probe. and the International Bovine Reference Family Panel (IBRP) [2]. Molecular reagents: human SAP-2 eDNA probe SAP4B (ATCC# Further studies of the linkage order among the Type I loci 59662). Filters were hybridized at 42~ in 50% formamide and assigned to BTA28 will reveal whether the syntenic conservation washed at low stringency (1 x SSC, 0.1% SDS at 55~ of these chromosomal regions between cattle and human reflects Method of allele detection: HindlII and PstI revealed two-allele conservation of linkage (gene order). Linkage data from other restriction fragment length polymorphisms (RFLPs) with frag- chromosomes [2] suggest the conservation of gene order between ments of 5.3 kb and 6.2 kb, and 3.8 kb and 1.8 kb, respectively cattle and humans may be extensively disrupted within conserved (Fig. 1). syntenic elements. Previously identified homologs: PSAP has been mapped to 10q21- 22 in humans [3], and to Chr 10 in mice [4]. Acknowledgments. This work was supported in part by the Texas Agri- Discussion: Sphingolipid activator proteins (SAPs), proteins cultural Experiment Station, by USDA NRI grant 92-37205-8048, USAID which stimulate reactions between sphingolipid substrates and spe- grant DHR 5566-G-00-0073-00 and by FPU grant (Ministerio de Educa- cific lysosomal acids, are derived from a single precursor, prosa- ci6n y Ciencia, Spain). posin (PSAP), by proteolytic processing. In order to establish the localization of this locus in cattle, References DNA from bovine-rodent hybrid cells was analyzed by Southern 1. Womack, J.E., Moll, Y.D. (1986). J. Hered. 77, 2-7. blotting and hybridization with the PSAP probe. BgIII-digested 2. Barendse, W., Armitage, S.M., Kossarek, L.M., Shalom, A., Kirkpa- bovine DNA revealed fragments that differed in size from the trick, B.W., Ryan, A.M., Clayton, D., Li, L., Neibergs, H.L., Zhang, N., fragments of hamster and mouse, and allowed assignment of PSAP Grosse, W.M., Weiss, J., Creighton, P., McCarthy, F., Ron, M., Teale, to bovine Chr 28 (previously syntenic group U29) with a concor- A.J., Fries, R., McGraw, R.A., Moore, S.S., Georges, M., Soller, M., Womack, J.E., Hetzel, D.J.S. (1994). Nature Genet, 6, 227-234. dancy of 100%. This assignment further defines a region of syn- 3. Fujibayashi, S., Kao, F.T., Jones, C., Morse, H., Law, M., Wenger, D.A. tenic conservation among bovine, human, and mouse. While the (1985). Am. J. Hum. Genet. 37, 741-748. HSA10, BTA28, MMU10 conservation now includes two loci 4. Kozak, C.A., Adamson, M.C., Horowitz, M. (1994). Genomics 23, [hexokinase 1 (HK1) and PSAP], the HSA10, BTA28 conservation 508--510. is more extensive (interstitial retinol-binding protein 3 (RBP3), PSAP, HK1 and plasminogen activator, urokinase type (PLAU). In addition, PSAP polymorphisms were analyzed in parents of Localization of the beta-nerve growth factor the IBRP. The informative families of the IBRP were genotyped and the data merged with the Cattle Genotypic Database. All pos- gene (NGFB) to bovine Chromosome 3 sible pairwise comparisons were performed, and linkage was de- tected to the locus D28S4. A multipoint analysis of PSAP was C. Elduque, 1 J.E. Womack 2 performed (CRI-MAP v2.4 SunOS) with the anonymous DNA loci D28S1, D28S2, and D28S4 and with RBP3, all previously reported 1Faeultad de Veterinaria, Universidad de Zaragoza, Miguel Servet 177, to map to Chr 28 [2]. All possible orders of these five loci were 50013 Zaragoza, Spain compared and the order RBP3-PSAP-D28S1-D28S4-D28S2 (log ZDepartment of Veterinary Pathobiology, Texas A&M University, College Station, Texas 77843, USA odds = 101.91) was better than PSAP-RBP3-D28S1-D28S4- D28S2 by a difference in log odds of 1.18. The next best order was Received: 3 July 1996 / Accepted: 27 August 1996 RBP3-D28S1-D28S4-D28S2-PSAP with a difference in log odds of 2.29. PSAP is likely to be towards the RBP3 side of the linkage- Species: Cattle group, but because of the lack of informative meioses a recombi- Locus name: Nerve growth factor, beta subunit nation fraction with RBP3 cannot be calculated. Locus symbol: NGFB Correspondence to: C. Elduque at Laboratoire de Genetique Biochimique Correspondence to: C. Elduque at Laboratoire de Genetique Blochimique et de Cytogenetique, INRA Jouy en Josas 78352, CEDEX France. et de Cytogenetique, INRA Jouy en Josas 78352, CEDEX France. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Mammalian Genome Springer Journals

Physical and genetic mapping of the prosaposin gene (PSAP) to bovine Chromosome 28

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Springer-Verlag
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Copyright © 1997 by Springer-Verlag
Subject
Life Sciences; Cell Biology; Anatomy; Zoology
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0938-8990
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1432-1777
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10.1007/s003359900622
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Abstract

Mammalian Genome 8, Brief Data Reports 73 Physical and genetic mapping of the prosaposin gene (PSAP) to bovine Chromosome 28 C. Elduque, 1 L. Li, 2 W. Barendse, 3 J.E. Womack 2 1Facultad de Veterinaria, Universidad de Zaragoza, Miguel Servet 177, 50013 Zaragoza, Spain 2Department of Veterinary Pathobiology, Texas A&M University, College Station, Texas 77843, USA 3CSIRO, Division of Tropical Animal Production, Research Road, University of Queensland, St. Lucia, Brisbane, 4072, Australia Received: 3 July 1996 / Accepted: 27 August 1996 Species: Cattle Locus name: Prosaposin Fig. 1. Segregation in two cattle Locus symbol: PSAP families of PSAP polymorphisms Map position: BTA28. Linkage was detected to the anonymous after digestion with HindlIl (A) and DNA polymorphism D28S4 (theta 0.22, Iodscore 4.50). PstI (B) and probed with a human Method of mapping: Southern analysis of somatic cell hybrids [1] PSAP probe. and the International Bovine Reference Family Panel (IBRP) [2]. Molecular reagents: human SAP-2 eDNA probe SAP4B (ATCC# Further studies of the linkage order among the Type I loci 59662). Filters were hybridized at 42~ in 50% formamide and assigned to BTA28 will reveal whether the syntenic conservation washed at low stringency (1 x SSC, 0.1% SDS at 55~ of these chromosomal regions between cattle and human reflects Method of allele detection: HindlII and PstI revealed two-allele conservation of linkage (gene order). Linkage data from other restriction fragment length polymorphisms (RFLPs) with frag- chromosomes [2] suggest the conservation of gene order between ments of 5.3 kb and 6.2 kb, and 3.8 kb and 1.8 kb, respectively cattle and humans may be extensively disrupted within conserved (Fig. 1). syntenic elements. Previously identified homologs: PSAP has been mapped to 10q21- 22 in humans [3], and to Chr 10 in mice [4]. Acknowledgments. This work was supported in part by the Texas Agri- Discussion: Sphingolipid activator proteins (SAPs), proteins cultural Experiment Station, by USDA NRI grant 92-37205-8048, USAID which stimulate reactions between sphingolipid substrates and spe- grant DHR 5566-G-00-0073-00 and by FPU grant (Ministerio de Educa- cific lysosomal acids, are derived from a single precursor, prosa- ci6n y Ciencia, Spain). posin (PSAP), by proteolytic processing. In order to establish the localization of this locus in cattle, References DNA from bovine-rodent hybrid cells was analyzed by Southern 1. Womack, J.E., Moll, Y.D. (1986). J. Hered. 77, 2-7. blotting and hybridization with the PSAP probe. BgIII-digested 2. Barendse, W., Armitage, S.M., Kossarek, L.M., Shalom, A., Kirkpa- bovine DNA revealed fragments that differed in size from the trick, B.W., Ryan, A.M., Clayton, D., Li, L., Neibergs, H.L., Zhang, N., fragments of hamster and mouse, and allowed assignment of PSAP Grosse, W.M., Weiss, J., Creighton, P., McCarthy, F., Ron, M., Teale, to bovine Chr 28 (previously syntenic group U29) with a concor- A.J., Fries, R., McGraw, R.A., Moore, S.S., Georges, M., Soller, M., Womack, J.E., Hetzel, D.J.S. (1994). Nature Genet, 6, 227-234. dancy of 100%. This assignment further defines a region of syn- 3. Fujibayashi, S., Kao, F.T., Jones, C., Morse, H., Law, M., Wenger, D.A. tenic conservation among bovine, human, and mouse. While the (1985). Am. J. Hum. Genet. 37, 741-748. HSA10, BTA28, MMU10 conservation now includes two loci 4. Kozak, C.A., Adamson, M.C., Horowitz, M. (1994). Genomics 23, [hexokinase 1 (HK1) and PSAP], the HSA10, BTA28 conservation 508--510. is more extensive (interstitial retinol-binding protein 3 (RBP3), PSAP, HK1 and plasminogen activator, urokinase type (PLAU). In addition, PSAP polymorphisms were analyzed in parents of Localization of the beta-nerve growth factor the IBRP. The informative families of the IBRP were genotyped and the data merged with the Cattle Genotypic Database. All pos- gene (NGFB) to bovine Chromosome 3 sible pairwise comparisons were performed, and linkage was de- tected to the locus D28S4. A multipoint analysis of PSAP was C. Elduque, 1 J.E. Womack 2 performed (CRI-MAP v2.4 SunOS) with the anonymous DNA loci D28S1, D28S2, and D28S4 and with RBP3, all previously reported 1Faeultad de Veterinaria, Universidad de Zaragoza, Miguel Servet 177, to map to Chr 28 [2]. All possible orders of these five loci were 50013 Zaragoza, Spain compared and the order RBP3-PSAP-D28S1-D28S4-D28S2 (log ZDepartment of Veterinary Pathobiology, Texas A&M University, College Station, Texas 77843, USA odds = 101.91) was better than PSAP-RBP3-D28S1-D28S4- D28S2 by a difference in log odds of 1.18. The next best order was Received: 3 July 1996 / Accepted: 27 August 1996 RBP3-D28S1-D28S4-D28S2-PSAP with a difference in log odds of 2.29. PSAP is likely to be towards the RBP3 side of the linkage- Species: Cattle group, but because of the lack of informative meioses a recombi- Locus name: Nerve growth factor, beta subunit nation fraction with RBP3 cannot be calculated. Locus symbol: NGFB Correspondence to: C. Elduque at Laboratoire de Genetique Biochimique Correspondence to: C. Elduque at Laboratoire de Genetique Blochimique et de Cytogenetique, INRA Jouy en Josas 78352, CEDEX France. et de Cytogenetique, INRA Jouy en Josas 78352, CEDEX France.

Journal

Mammalian GenomeSpringer Journals

Published: Mar 23, 2009

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