Arch Virol (2002) 147: 2075–2087
Phylogenetic relationships of dengue-1 viruses from Argentina
, J. Meissner
, J. C. Manzur Caffarena
, D. Enria
and S. St. Jeor
Department of Microbiology, University of Nevada, Reno, U.S.A.
Nevada Genomics Center, University of Nevada, Reno, U.S.A.
Instituto de Medicina Tropical, Laboratorio Central de Salud P´ublica,
Ministerio de Salud P´ublica y Bienestar Social, Florida Asunci´on, Paraguay
Instituto Nacional de Enfermedades Virales Humanas
“Dr. J. I. Maiztegui”-INEVH-ANLIS, Ministerio Nacional de Salud,
Received May 6, 2002; accepted July 10, 2002
Published online September 18, 2002
Summary. We sequenced the Capsid-pre Membrane (C/prM) and the Envelope-
from South America. This included 12 Argentinean and 11 Paraguayan DEN-1
strainsisolatedin2000plus a Paraguayanstrain isolated in1988.These sequences
were compared with published sequences of DEN-1 isolated worldwide to deter-
mine the origin of these isolates. Pairwise comparisons of strains from Paraguay
andArgentina revealed a nucleotide divergence of 0–5% in the E/NS1 region and
0–3% in the C/prM region. Our results showed that these viruses belong to the
same genotype, but can be separated into two clades. Interestingly, both clades
circulated simultaneously in the same geographic area during the 2000 outbreaks.
Amino acid differences were found between both clades in the C/prM region at
position 100 (Lys vs.Arg) and in the E/NS1 region at positions 722 (Ala vs. Thr).
Although the geographic movement of DEN-1 virus can not be unequivocally
traced from the genetic relationship determined here, our results suggest that the
recent epidemics in Argentina and Paraguay were due to the re-emergence of a
previously circulating strain, or to the virus circulating unnoticed, rather than to
the introduction of a new genotype.
Dengue (DEN) virus infections are a serious public health problem in all tropical
and subtropical areas of the world, with up to 100 million infections occurring