Arch Virol (1997) 142: 1979±1999
Phylogenetic analysis of the Potyviridae with emphasis
on legume-infecting potyviruses
P. H. Berger
*, S. D. Wyatt
*, P. J. Shiel
, M. J. Silbernagel
, K. Druffel
and G. I. Mink
Department of Plant, Soil and Entomological Sciences, University of Idaho, Moscow,
Department of Plant Pathology, Washington State University, Pullman, Washington,
IAREC, Washington State University, Prosser, Washington, U.S.A.
Accepted June 3, 1997
Summary. The 3
-terminal nucleotide sequences of thirteen authenticated
strains of bean common mosaic virus (BCMV) and one strain of bean common
mosaic necrosis virus (BCMNV) were obtained. The regions sequenced
included the coat protein coding sequence and 3
-end non-coding region. These
data, combined with sequence information from other legume-infecting
potyviruses and the Potyviridae were used for phylogenetic analysis. Evidence
is provided for delineation of BCMNV as distinct from BCMV and the inclusion
of azuki mosaic, dendrobium mosaic, blackeye cowpea mosaic, and peanut
stripe viruses as strains of BCMV. This relationship de®nes the members of the
BCMV and BCMNV subgroups. These data also provide a basis upon which to
de®ne virus strains, in combination with biological data. Other aspects and
implications of legume-infecting potyvirus phylogenetics are discussed.
Of the plant viruses known to infect Phaseolus vulgaris L., bean common
mosaic potyvirus (BCMV) strains are perhaps the most economically
important. In addition to being aphid-borne, BCMV is also seedborne and, as
such, can signi®cantly impact bean crops world wide. BCMV represents a
complex of virus strains and now appears to include at least two distinct viruses
[45, 50, 53, 55]. Comprehensive identi®cation of these entities can be dif®cult.
The initial and still de®nitive identi®cation depends on responses of a series of
P. vulgaris host differential cultivars ®rst described by Drifhout . Currently,
seven pathogroups out of a predicted 16 have been identi®ed .
* The ®rst two authors contributed equally to this work.