Phylogenetic analysis of porcine reproductive and respiratory syndrome virus isolates from Northern Ireland

Phylogenetic analysis of porcine reproductive and respiratory syndrome virus isolates from... To investigate the genetic diversity of porcine reproductive and respiratory syndrome virus (PRRSV) in Northern Ireland, the ORF5 gene from nine field isolates was sequenced and phylogenetically analysed. The results revealed relatively high diversity amongst isolates, with 87.6-92.2% identity between farms at the nucleotide level and 84.1-93.5% identity at the protein level. Phylogenetic analysis confirmed that all nine isolates belonged to the European (type 1) genotype and formed a cluster within the subtype 1 subgroup. This study provides the first report on PRRSV isolate diversity in Northern Ireland. Porcine reproductive and respiratory syndrome virus island of Ireland is geographically isolated, we hypothesised (PRRSV), the causative agent of porcine reproductive that Northern Ireland (NI) may have circulating strains of and respiratory syndrome (PRRS), is a small, enveloped, virus that are different from those found in Great Britain positive-sense, single-stranded RNA virus belonging to the and the rest of Europe. The genotype of the virus has obvi- genus Arterivirus. It is responsible for inducing reproductive ous implications for diagnostics and vaccination, with only failure in sows and respiratory disease in growing pigs [1]. limited protection afforded against heterologous strains. In Two genotypes of PRRSV are recognised internationally: this study, the ORF5 http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Archives of Virology Springer Journals

Phylogenetic analysis of porcine reproductive and respiratory syndrome virus isolates from Northern Ireland

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Publisher
Springer Vienna
Copyright
Copyright © 2018 by Springer-Verlag GmbH Austria, part of Springer Nature
Subject
Biomedicine; Virology; Medical Microbiology; Infectious Diseases
ISSN
0304-8608
eISSN
1432-8798
D.O.I.
10.1007/s00705-018-3886-7
Publisher site
See Article on Publisher Site

Abstract

To investigate the genetic diversity of porcine reproductive and respiratory syndrome virus (PRRSV) in Northern Ireland, the ORF5 gene from nine field isolates was sequenced and phylogenetically analysed. The results revealed relatively high diversity amongst isolates, with 87.6-92.2% identity between farms at the nucleotide level and 84.1-93.5% identity at the protein level. Phylogenetic analysis confirmed that all nine isolates belonged to the European (type 1) genotype and formed a cluster within the subtype 1 subgroup. This study provides the first report on PRRSV isolate diversity in Northern Ireland. Porcine reproductive and respiratory syndrome virus island of Ireland is geographically isolated, we hypothesised (PRRSV), the causative agent of porcine reproductive that Northern Ireland (NI) may have circulating strains of and respiratory syndrome (PRRS), is a small, enveloped, virus that are different from those found in Great Britain positive-sense, single-stranded RNA virus belonging to the and the rest of Europe. The genotype of the virus has obvi- genus Arterivirus. It is responsible for inducing reproductive ous implications for diagnostics and vaccination, with only failure in sows and respiratory disease in growing pigs [1]. limited protection afforded against heterologous strains. In Two genotypes of PRRSV are recognised internationally: this study, the ORF5

Journal

Archives of VirologySpringer Journals

Published: May 29, 2018

References

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