Arch Virol (2001) 146: 1775–1785
Phylogenetic analysis of avian paramyxovirus
1 strains isolated in Finland
, C. Ek-Kommonen
, R. Manvell
, and L. Kinnunen
National Veterinary and Food Research Institute, Department of Virology,
Avian Virology, Veterinary Laboratories Agency (Weybridge), New Haw, U.K.
National Public Health Institute, Helsinki, Finland
Accepted June 11, 2001
Summary. Eight strains of avian paramyxovirus type 1 (PMV-1) isolated in Fin-
land during the last 3 decades were studied with reverse transcriptase polymerase
chain reaction (RT-PCR) and subsequent sequence analysis of the region of 208
nucleotides covering the fusion (F) protein cleavage site. Both genetic and anti-
genic heterogeneity of the strains was signiﬁcant. Direct epidemiological links
between strains isolated during successive outbreaks, and also between strains
isolated from wild fauna and from poultry or captive birds, were seen in this
study. These results also support the previously published view that wild water-
fowl serve as a reservoir for the apathogenic or low-pathogenic strains, allowing
them to evolve further into pathogenic strains.
Avian paramyxoviruses (APMV) belong to the Rubulavirus genus of Paramyxo-
viridae in the order Mononegavirales . Their genome is an approximately 15-
kilobase-long, negative-sense, single-stranded RNA molecule having 6 genes that
code for nucleo-, phospho-, matrix-, fusion-, haemagglutination- neuraminidase-
and polymerase proteins . Recently, it has been shown that Newcastle dis-
ease virus (NDV) is only distantly related to the other members of the genus
Rubulavirus and should be considered as a member of a new genus within the
subfamily Paramyxovirinae .
GenBank accession numbers: AY034794–AY034801.