Phototropin 1 is required for high-fluence blue-light-mediated mRNA destabilization

Phototropin 1 is required for high-fluence blue-light-mediated mRNA destabilization Irradiation of etiolated wild-type Arabidopsis thaliana seedlings with a single pulse of blue (B) light with a total fluence equal to that of 1 min of sunlight causes the destabilization of nuclear-encoded Lhcb transcripts. Transcript destabilization is not observed in phototropin1 (phot1, formerly nph1) mutant seedlings, indicating that phot1 is likely the photoreceptor mediating this response. Destabilization is also absent in nph3 mutants, but occurs normally in nph4 mutants. The rates of Lhcb transcription and B low-fluence-induced Lhcb transcript accumulation are normal in phot1 seedlings, confirming that phot1 regulates destabilization, not a change in transcription. A similar pattern of regulation is observed for the chloroplast-encoded rbcL transcript. The lack of destabilization of a second chloroplast encoded transcript, psbD, indicates that the phot1/B-high-fluence system does not result in a general destabilization of all chloroplast transcripts. Localized sequence similarity between the Lhcb 5′-UTR and the rbcL 3′-UTR suggests a similar mechanism of destabilization even though the two transcripts are located in different sub-cellular compartments. The high-fluence threshold of phot1-mediated RNA destabilization contrasts with the low-fluence threshold required for phot1-directed first-positive phototropic curvature. This study indicates that phot1, like phytochrome, can discriminate between several fluence ranges and direct responses in specific tissues or different sub-cellular compartments. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Plant Molecular Biology Springer Journals

Phototropin 1 is required for high-fluence blue-light-mediated mRNA destabilization

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Publisher
Springer Journals
Copyright
Copyright © 2003 by Kluwer Academic Publishers
Subject
Life Sciences; Biochemistry, general; Plant Sciences; Plant Pathology
ISSN
0167-4412
eISSN
1573-5028
D.O.I.
10.1023/A:1022393406204
Publisher site
See Article on Publisher Site

Abstract

Irradiation of etiolated wild-type Arabidopsis thaliana seedlings with a single pulse of blue (B) light with a total fluence equal to that of 1 min of sunlight causes the destabilization of nuclear-encoded Lhcb transcripts. Transcript destabilization is not observed in phototropin1 (phot1, formerly nph1) mutant seedlings, indicating that phot1 is likely the photoreceptor mediating this response. Destabilization is also absent in nph3 mutants, but occurs normally in nph4 mutants. The rates of Lhcb transcription and B low-fluence-induced Lhcb transcript accumulation are normal in phot1 seedlings, confirming that phot1 regulates destabilization, not a change in transcription. A similar pattern of regulation is observed for the chloroplast-encoded rbcL transcript. The lack of destabilization of a second chloroplast encoded transcript, psbD, indicates that the phot1/B-high-fluence system does not result in a general destabilization of all chloroplast transcripts. Localized sequence similarity between the Lhcb 5′-UTR and the rbcL 3′-UTR suggests a similar mechanism of destabilization even though the two transcripts are located in different sub-cellular compartments. The high-fluence threshold of phot1-mediated RNA destabilization contrasts with the low-fluence threshold required for phot1-directed first-positive phototropic curvature. This study indicates that phot1, like phytochrome, can discriminate between several fluence ranges and direct responses in specific tissues or different sub-cellular compartments.

Journal

Plant Molecular BiologySpringer Journals

Published: Oct 7, 2004

References

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