Photobleached-fluorescence imaging of microflows

Photobleached-fluorescence imaging of microflows A photobleached-fluorescence imaging technique for visualizing microscale flow fields and obtaining molecular diffusion and advection information has been developed. The technique tracks fluorophores in the region of a photobleached line in a planar microdevice and yields quantitative diffusive and advective transport data. Visualizations of two- and weakly three-dimensional electroosmotically and pressure-driven fluid flow fields are demonstrated using the photobleaching of fluorescein and fluorescein-dextran conjugates. Photobleached-fluorescence imaging tracks undisturbed fluorophores, functions in polymer and glass microfluidic devices, can take advantage of fluorescent conjugates present in biochemical assays, and has a photobleached region that is flow independent. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Experiments in Fluids Springer Journals

Photobleached-fluorescence imaging of microflows

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Publisher
Springer-Verlag
Copyright
Copyright © 2002 by Springer-Verlag
Subject
Engineering; Engineering Fluid Dynamics; Fluid- and Aerodynamics; Engineering Thermodynamics, Heat and Mass Transfer
ISSN
0723-4864
eISSN
1432-1114
D.O.I.
10.1007/s00348-002-0486-8
Publisher site
See Article on Publisher Site

Abstract

A photobleached-fluorescence imaging technique for visualizing microscale flow fields and obtaining molecular diffusion and advection information has been developed. The technique tracks fluorophores in the region of a photobleached line in a planar microdevice and yields quantitative diffusive and advective transport data. Visualizations of two- and weakly three-dimensional electroosmotically and pressure-driven fluid flow fields are demonstrated using the photobleaching of fluorescein and fluorescein-dextran conjugates. Photobleached-fluorescence imaging tracks undisturbed fluorophores, functions in polymer and glass microfluidic devices, can take advantage of fluorescent conjugates present in biochemical assays, and has a photobleached region that is flow independent.

Journal

Experiments in FluidsSpringer Journals

Published: Oct 14, 2002

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