Access the full text.
Sign up today, get DeepDyve free for 14 days.
E. Barbarotto, Thomas Schmittgen, G. Calin (2007)
MicroRNAs and cancer: Profile, profile, profileInternational Journal of Cancer, 122
A. Fusco, G. Viglietto, M. Santoro (2005)
Point mutation in GRIM-19: a new genetic lesion in Hurthle cell thyroid carcinomasBritish Journal of Cancer, 92
Ranran Zhang, Heather Hardin, Jidong Chen, Zhenying Guo, R. Lloyd (2016)
Non-Coding RNAs in Thyroid CancerEndocrine Pathology, 27
J. Mattick, J. Rinn (2015)
Discovery and annotation of long noncoding RNAsNature Structural &Molecular Biology, 22
V. Máximo, Tiago Botelho, J. Capela, P. Soares, Jorge Lima, A. Taveira, T. Amaro, A. Barbosa, A. Preto, H. Harach, Dillwyn Williams, M. Sobrinho-Simões (2005)
Somatic and germline mutation in GRIM-19, a dual function gene involved in mitochondrial metabolism and cell death, is linked to mitochondrion-rich (Hürthle cell) tumours of the thyroidBritish Journal of Cancer, 92
M. Dettmer, A. Vogetseder, M. Durso, H. Moch, P. Komminoth, A. Perren, Y. Nikiforov, M. Nikiforova (2013)
MicroRNA expression array identifies novel diagnostic markers for conventional and oncocytic follicular thyroid carcinomas.The Journal of clinical endocrinology and metabolism, 98 1
V. Máximo, Jorge Lima, H. Prazeres, P. Soares, M. Sobrinho-Simões (2012)
The biology and the genetics of Hurthle cell tumors of the thyroid.Endocrine-related cancer, 19 4
Heather Hardin, Zhenying Guo, Weihua Shan, Celina Montemayor-Garcia, S. Asioli, Xiao‐Min Yu, A. Harrison, Herbert Chen, R. Lloyd (2014)
The roles of the epithelial-mesenchymal transition marker PRRX1 and miR-146b-5p in papillary thyroid carcinoma progression.The American journal of pathology, 184 8
TG Kroll, P Saraf, L Pecciarini, CJ Chen, E Mueller, BM Spiegelman, JA Fletcher (2000)
PAX8-PPARgamma1 fusion oncogene in human thyroid carcinomaScience, 289
M. Petrulea, T. Plantinga, J. Smit, C. Georgescu, R. Netea-Maier (2015)
PI3K/Akt/mTOR: A promising therapeutic target for non-medullary thyroid carcinoma.Cancer treatment reviews, 41 8
Kroll Tg, P. Sarraf, L. Pecciarini, Chang-jie Chen, E. Mueller, B. Spiegelman, J. Fletcher (2000)
PAX8-PPARgamma1 fusion oncogene in human thyroid carcinoma [corrected].Science, 289 5483
Z Guo, H Hardin, S Asioli, A Righi, F Maletta, A Sapino, RV Lloyd (2015)
In situ hybridization analysis of miR-146b-5p and miR-21 expression in thyroid carcinoma and prognostic implicationsEndocr Pathol, 26
Heather Hardin, Xiao‐Min Yu, A. Harrison, C. Larraín, Ranran Zhang, Jidong Chen, Herbert Chen, R. Lloyd (2016)
Generation of Novel Thyroid Cancer Stem-Like Cell Clones: Effects of Resveratrol and Valproic Acid.The American journal of pathology, 186 6
Heather Hardin, Ranran Zhang, Holly Helein, D. Buehler, Zhenying Guo, R. Lloyd (2017)
The evolving concept of cancer stem-like cells in thyroid cancer and other solid tumorsLaboratory Investigation, 97
Zhenying Guo, Heather Hardin, Celina Montemayor-Garcia, S. Asioli, A. Righi, F. Maletta, A. Sapino, R. Lloyd (2015)
In Situ Hybridization Analysis of miR-146b-5p and miR-21 in Thyroid Nodules: Diagnostic ImplicationsEndocrine Pathology, 26
Jing Liu, Robert Brown (2011)
Morphoproteomics demonstrates activation of mammalian target of rapamycin pathway in papillary thyroid carcinomas with nuclear translocation of MTOR in aggressive histological variantsModern Pathology, 24
T. Kroll, P. Sarraf, L. Pecciarini, Chang-jie Chen, E. Mueller, B. Spiegelman, J. Fletcher (2000)
PAX8-PPARγ1 Fusion in Oncogene Human Thyroid CarcinomaScience, 289
Ranran Zhang, Heather Hardin, Wei Huang, Jidong Chen, S. Asioli, A. Righi, F. Maletta, A. Sapino, R. Lloyd (2017)
MALAT1 Long Non-coding RNA Expression in Thyroid Tissues: Analysis by In Situ Hybridization and Real-Time PCREndocrine Pathology, 28
Oncocytic (Hürthle cell) and follicular neoplasms are related thyroid tumors with distinct molecular profiles. Diagnostic criteria separating adenomas and carcinomas for these two types of neoplasms are similar, but there may be some differences in the biological behavior of Hürthle cell and follicular carcinomas. Recent studies have shown that noncoding RNAs may have diagnostic and prognostic utility in separating benign and malignant Hürthle cell and follicular neoplasms. In this study, we examined expression of various noncoding RNAs including metastasis associated lung adenocarcinoma transcript 1 (MALAT1) and miR-RNA-885-5p (miR-885) in distinguishing between benign and malignant neoplasms. In addition, the expression of phosphorylated mechanistic receptor of rapamycin (p-mTOR) was also analyzed in these two groups of tumors. Tissue microarrays (TMAs) with archived tissue samples were analyzed using in situ hybridization (ISH) for MALAT1 and miR-885 and immunohistochemistry (IHC) for p-mTOR. Quantitative reverse transcriptase polymerase chain reaction (qRT-PCR) was also performed on a subset of the cases.MALAT1 and miR-885 were increased in all neoplastic groups compared to the normal thyroid tissues (p < 0.05). MALAT1 was more highly expressed in HCCs compared to FTCs, although the differences were not statistically significant (p = 0.06). MiR-885 was expressed at similar levels in FTCs and HCCs. P-mTOR protein was more highly expressed in FTCs than in HCCs (p<0.001). qRT-PCR analysis of noncoding RNAs supported the ISH findings. These results indicate that the noncoding RNAs MALAT1 and miR-885 show increased expression in neoplastic follicular and Hürthle cell thyroid neoplasms compared to normal thyroid tissues. P-mTOR was most highly expressed in FTC but was also increased in HCC, suggesting that drugs targeting this pathway may be useful for treatment of tumors unresponsive to conventional therapies.
Endocrine Pathology – Springer Journals
Published: Jun 28, 2017
Read and print from thousands of top scholarly journals.
Already have an account? Log in
Bookmark this article. You can see your Bookmarks on your DeepDyve Library.
To save an article, log in first, or sign up for a DeepDyve account if you don’t already have one.
Copy and paste the desired citation format or use the link below to download a file formatted for EndNote
Access the full text.
Sign up today, get DeepDyve free for 14 days.
All DeepDyve websites use cookies to improve your online experience. They were placed on your computer when you launched this website. You can change your cookie settings through your browser.