1021-4437/02/4902- $27.00 © 2002
Russian Journal of Plant Physiology, Vol. 49, No. 2, 2002, pp. 286–289. Translated from Fiziologiya Rastenii, Vol. 49, No. 2, 2002, pp. 320–323.
Original Russian Text Copyright © 2002 by Markovskaya, Talanova, Olimpienko, Lebedeva, Tikhov.
Mutant plants are extensively used in physiological
and biochemical research on the functioning of pig-
ment–protein complexes in plant photosystems [1–4].
Mutants with different photosystem defects serve as a
tool to divide a complex physiological process into
components and throw light on its intrinsic mecha-
nisms. This approach was used to study the assembling
of pigment–protein complexes and their regulation [3,
4]. On the other hand, the physiology of any new
mutant phenotype is often unknown and requires a sep-
arate study. For instance, chlorophyll-defective mutants
, ranging from deep-green to color-
less phenotypes, have been obtained in the Genetics
Laboratory, Institute of Biology, Karelian Research
Center, Russian Academy of Sciences [1, 5]. These
mutations are expressed in seedling phenotypes only at
high temperatures, which, according to the authors, is
related to the inactivation of suppressor systems. The
100% vitality of the suppressed chlorophyll-defective
mutants, and consequent reproducibility of effects from
generation to generation, is an important experimental
advantage of these mutants compared to unsuppressed
nuclear mutants with pigment defects.
MATERIALS AND METHODS
We used seedlings of
Karel’skaya (control, wild type) and mutant lines (ﬁfth
generation), obtained with various mutagens in the
Genetics Laboratory (Institute of Biology, Karelian
Research Center, RAS) . Seeds were germinated in a
growth cabinet at 70% humidity in rolls between two
vertical sheets of ﬁlter paper moistened from the sup-
porting trays according to the technique used for grow-
The design of the experiment included three series.
(1) Seeds of the control and experimental plants
were germinated under optimal temperature conditions
C) and an illuminance of 8 klx for 10 days until the
appearance of the second leaf. Then, the seedlings were
sampled and analyzed.
(2) Seeds of the control and experimental lines
(mutant lines 2, 4, and 6) were germinated under high
C) and at an illuminance of 8 klx for
10 days until the appearance of the second true leaf.
This treatment revealed the temperature-dependent
chlorophyll-deﬁcient phenotypes, which differed in
color. The dark green, pale green, yellow-green, and
yellow phenotypes were designated as
, respectively . The
seedlings of variously colored phenotypes were sam-
pled and analyzed.
Special experiments revealed that the seedlings of
all phenotypes and different genotypes, grown at 35
for 10 days, gave rise to green plants when transferred
into the experimental plots in open air.
(3) Seeds of control and experimental plants were
germinated at 35
C and an illuminance of 8 klx for
10 days. Then, the seedlings were kept at 25
C for 4
days and analyzed.
Phenomenon of Temperature-Dependent Chlorophyll Deficiency
E. F. Markovskaya*, T. Yu. Talanova*, G. S. Olimpienko**,
O. N. Lebedeva**, and P. V. Tikhov**
*Petrozavodsk State University
**Institute of Biology, Karelian Research Center, Russian Academy of Sciences,
Pushkinskaya ul. 11, Petrozavodsk, 185610 Russia;
Received July 20, 2001
—The composition and amount of pigments were studied in temperature-dependent chlorophyll-deﬁ-
cient seedlings of wild type (control) and several mutant lines of
Huds. at room (25
C) temperatures. In seedlings of all mutant lines grown at 25
content was lower and
the concentration of carotenoids was higher than in control seedlings. At 35
C, the concentration of all pigments
decreased in a row from
phenotypes, and this trend was retained when the temperature
was lowered to 25
C. The phenotype
completely lacked violaxanthin and neoxanthin. The observed
effects are related to the protective dissipative function of the xanthophyll cycle.
Key words: Festuca pratensis - mutant lines - xanthophyll-deﬁcient phenotypes - pigments - carotenoids
: PSI—photosystem I; PSII—photosystem II.