Pervasive interactions of Sa and Sb loci cause high pollen sterility and abrupt changes in gene expression during meiosis that could be overcome by double neutral genes in autotetraploid rice

Pervasive interactions of Sa and Sb loci cause high pollen sterility and abrupt changes in gene... Background: Intersubspecific autotetraploid rice hybrids possess high hybrid vigor; however, low pollen fertility is a critical hindrance in its commercial utilization. Our previous study demonstrated that polyploidy could increase the multi-loci interaction and cause high pollen abortion in autotetraploid rice hybrids. However, there is little known about the critical role of pollen sterility locus or loci in the intersubspecific hybrids. We developed autotetraploid i i j j rice hybrids harboring heterozygous genotypes (S S S S ) at different pollen sterility loci by using the near isogenic n n lines of Taichung65-4×. Moreover, autotetraploid lines carrying double neutral genes, Sa and Sb , were used to assess their effect on fertility restoration. Results: Cytological studies showed that the deleterious genetic interactions at Sa and Sb pollen sterility loci resulted in higher pollen sterility (76.83%) and abnormal chromosome behavior (24.59%) at metaphase I of meiosis in autotetraploid rice hybrids. Transcriptome analysis revealed 1092 differentially expressed genes (DEG) in a hybrid with the pervasive interactions at Sa and Sb pollen sterility loci, and most of the genes (about 83%) exhibited down regulation. Of the DEG, 60 were associated with transcription regulation and 18 genes were annotated as meiosis- related genes. Analysis http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Rice Springer Journals

Pervasive interactions of Sa and Sb loci cause high pollen sterility and abrupt changes in gene expression during meiosis that could be overcome by double neutral genes in autotetraploid rice

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Publisher
Springer US
Copyright
Copyright © 2017 by The Author(s).
Subject
Life Sciences; Plant Sciences; Plant Genetics and Genomics; Plant Breeding/Biotechnology; Agriculture; Plant Ecology
ISSN
1939-8425
eISSN
1939-8433
D.O.I.
10.1186/s12284-017-0188-8
Publisher site
See Article on Publisher Site

Abstract

Background: Intersubspecific autotetraploid rice hybrids possess high hybrid vigor; however, low pollen fertility is a critical hindrance in its commercial utilization. Our previous study demonstrated that polyploidy could increase the multi-loci interaction and cause high pollen abortion in autotetraploid rice hybrids. However, there is little known about the critical role of pollen sterility locus or loci in the intersubspecific hybrids. We developed autotetraploid i i j j rice hybrids harboring heterozygous genotypes (S S S S ) at different pollen sterility loci by using the near isogenic n n lines of Taichung65-4×. Moreover, autotetraploid lines carrying double neutral genes, Sa and Sb , were used to assess their effect on fertility restoration. Results: Cytological studies showed that the deleterious genetic interactions at Sa and Sb pollen sterility loci resulted in higher pollen sterility (76.83%) and abnormal chromosome behavior (24.59%) at metaphase I of meiosis in autotetraploid rice hybrids. Transcriptome analysis revealed 1092 differentially expressed genes (DEG) in a hybrid with the pervasive interactions at Sa and Sb pollen sterility loci, and most of the genes (about 83%) exhibited down regulation. Of the DEG, 60 were associated with transcription regulation and 18 genes were annotated as meiosis- related genes. Analysis

Journal

RiceSpringer Journals

Published: Dec 2, 2017

References

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