Persistence of Coxsackievirus B4 infection in rhabdomyosarcoma cells for 30 months

Persistence of Coxsackievirus B4 infection in rhabdomyosarcoma cells for 30 months A persistent infection of rhabdomyosarcoma (RD) cells by Coxsackie B4 virus (CBV-4) was established. The persistently infected RD (piRD) cells have been maintained for over 130 passages (30 months) and have released virus continuously without cellular destruction. The production of infectious virus declined three times during the study. After the first decline (third week post infection) a viral variant with a littered cytopathic effect (CPE) and a marked delayed replication cycle on Green Monkey Kidney (GMK) cells, replaced the original viral population. 100-fold diluted cell cultures were recovered from the piRD cells at the 48 th and 104 th passage. All 96 cultures from the former whereas 72% from the second dilution showed virus production when tested on GMK cells. Using a streptavidin/biotin immune-staining assay all piRD cells were positively stained. Test for ts mutants showed that the persistence of the CBV-4 strain was not dependent upon incubation temperature and addition of the antiviral compound disoxaril did not cure the piRD cells. Archives of Virology Springer Journals

Persistence of Coxsackievirus B4 infection in rhabdomyosarcoma cells for 30 months

Loading next page...
Copyright © Wien by 1999 Springer-Verlag/
Publisher site
See Article on Publisher Site

There are no references for this article.

You’re reading a free preview. Subscribe to read the entire article.

DeepDyve is your
personal research library

It’s your single place to instantly
discover and read the research
that matters to you.

Enjoy affordable access to
over 12 million articles from more than
10,000 peer-reviewed journals.

All for just $49/month

Explore the DeepDyve Library

Unlimited reading

Read as many articles as you need. Full articles with original layout, charts and figures. Read online, from anywhere.

Stay up to date

Keep up with your field with Personalized Recommendations and Follow Journals to get automatic updates.

Organize your research

It’s easy to organize your research with our built-in tools.

Your journals are on DeepDyve

Read from thousands of the leading scholarly journals from SpringerNature, Elsevier, Wiley-Blackwell, Oxford University Press and more.

All the latest content is available, no embargo periods.

See the journals in your area

Monthly Plan

  • Read unlimited articles
  • Personalized recommendations
  • No expiration
  • Print 20 pages per month
  • 20% off on PDF purchases
  • Organize your research
  • Get updates on your journals and topic searches


Start Free Trial

14-day Free Trial

Best Deal — 39% off

Annual Plan

  • All the features of the Professional Plan, but for 39% off!
  • Billed annually
  • No expiration
  • For the normal price of 10 articles elsewhere, you get one full year of unlimited access to articles.



billed annually
Start Free Trial

14-day Free Trial