Peroxidase-like activity of cytochrome c is significantly higher in the presence of anionic surfactants, sodium bis(2-ethylhexyl)sulfosuccinate (AOT) and sodium n-dodecyl sulphate (SDS). The enhancement is due to the higher reaction rate of the cytochrome c with H2O2, which is the rate determining step in the peroxidase cycle of cytochrome c. An intermediate formed in the cycle rapidly oxidizes the substrates or reacts with further molecule(s) of H2O2, causing heme degradation. Thus, the specific microenvironment provided by anionic surfactants facilitates also degradation of heme iron by H2O2.
Research on Chemical Intermediates – Springer Journals
Published: Oct 7, 2004
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