Pectate lyase gene expression and enzyme activity in ripening banana fruit

Pectate lyase gene expression and enzyme activity in ripening banana fruit Two distinct cDNA clones showing sequence homology to higher-plant pectate lyase (Pel) genes were isolated from ripening banana fruits. The transcripts were detected only in fruit tissue and both were strongly ripening-related. Yeast transformation with the most highly expressed Pel clone produced a recombinant protein with pectate lyase activity, demonstrating that this sequence was likely to encode a pectate lyase protein in planta. An assay developed for measuring the action of the endogenous enzyme from banana pulp tissue revealed a significant increase in calcium-dependent pectate lyase activity during ripening. The enhanced levels of enzyme activity corresponded with an increase in soluble polyuronides from banana pulp. Plant Molecular Biology Springer Journals

Pectate lyase gene expression and enzyme activity in ripening banana fruit

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Kluwer Academic Publishers
Copyright © 2003 by Kluwer Academic Publishers
Life Sciences; Biochemistry, general; Plant Sciences; Plant Pathology
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