Pathology-specific molecular profiles of saliva in patients with multiple
dental caries—potential application for predictive, preventive
and personalised medical services
Received: 15 February 2018 /Accepted: 5 April 2018 /Published online: 25 April 2018
European Association for Predictive, Preventive and Personalised Medicine (EPMA) 2018
Background Improving the quality of life is part of the global agenda. The focus is predominantly on prevention of socially
significant diseases. Combating dental caries-related diseases is a top priority as it has a huge impact on people’ssociallives.
Therefore, the purpose of the work was to study the changes in the molecular composition of saliva from subjects with multiple
caries lesions using spectroscopic methods of analysis to identify potential tissue markers of caries development for predictive,
preventive and personalised medical services.
Objectives and methods The molecular composition of mixed saliva (oral fluid) from subjects with and without multiple caries
was analysed with the use of spectroscopic techniques, FTIR with synchrotron radiation for the excitation. The IR spectra of the
oral fluid as well as the calculated mineral-organic, carbon-phosphate, Amide II/Amide I and protein/thiocyanate ratios were
compared between subjects with and without multiple caries.
Results This complex analysis of the obtained experimental data determined that the molecular composition of the oral fluid from
those with multiple caries differed from those without caries; the organic-mineral balance in the oral fluid of those with multiple
caries shifted towards a reduction in the mineral complexes, accompanied by an increase in the organic component. The
thiocyanate content increased more than twofold, accompanied by increased carboxyl groups of esters, lipids and carbohydrates.
Conclusion The detected features in the IR spectra of mixed saliva as well as the calculated changes in the ratios between organic
and inorganic components can be used as biomarkers of cariogenesis in the oral cavity, as a diagnostic criterion in the analysis of
the oral fluid samples.
Personalised medical service
Early prevention of diseases using precise monitoring
methods is a key paradigm in healthcare . The focus is
predominantly on socially significant diseases with dental
caries-related diseases being one of the most important ones
due to their huge impact on people’s social lives . The
analysis of modern publication on the development of
cutting-edge medical technologies [3, 4], i.e. on the ap-
proaches to early caries prevention [5–7], suggests that the
topic is a national top priority.
Therefore, a predictive medicine approach to prevent caries
at the stage that is not visually observed is currently one of the
leading directions in fundamental medicine and it is directly
connected to the use of a wider range of physical methods for
monitoring and analysis that are employed in tools for pathol-
ogy prevention [5, 8, 9].
It has been established that saliva represents one of the
most informative fluids of human diseases [10, 11]. Indeed,
proteins, lipids, immunoglobulins, enzymes and various
* Pavel Seredin
Present address: Department of Solid State Physics and
Nanostructures, Voronezh State University, University sq. 1,
Voronezh 394006, Russia
Department of Pediatric Dentistry with Orthodontia, Voronezh State
Medical University, Studentcheskaya st. 11, Voronezh 394006,
Australian Synchrotron (Synchrotron Light Source Australia Pty
LTD), 800 Blackburn Rd, Clayton, VIC 3168, Australia
EPMA Journal (2018) 9:195–203