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M. Turunen, Hanna Puhakka, J. Koponen, M. Hiltunen, J. Rutanen, O. Leppänen, Anna-Mari Turunen, A. Närvänen, A. Newby, A. Baker, S. Ylä-Herttuala (2002)
Peptide-retargeted adenovirus encoding a tissue inhibitor of metalloproteinase-1 decreases restenosis after intravascular gene transfer.Molecular therapy : the journal of the American Society of Gene Therapy, 6 3
Shamima Akhtar, F. Gremse, F. Kiessling, C. Weber, A. Schober (2013)
CXCL12 Promotes the Stabilization of Atherosclerotic Lesions Mediated by Smooth Muscle Progenitor Cells in Apoe-Deficient MiceArteriosclerosis, Thrombosis, and Vascular Biology, 33
T. Shimaoka, N. Kume, M. Minami, K. Hayashida, H. Kataoka, T. Kita, S. Yonehara (2000)
Molecular Cloning of a Novel Scavenger Receptor for Oxidized Low Density Lipoprotein, SR-PSOX, on Macrophages*The Journal of Biological Chemistry, 275
Gen Tanaka, I. Nakase, Yasunori Fukuda, R. Masuda, S. Oishi, Kazuya Shimura, Yoshimasa Kawaguchi, Tomoka Takatani-Nakase, Ű. Langel, A. Gräslund, K. Okawa, M. Matsuoka, N. Fujii, Y. Hatanaka, S. Futaki (2012)
CXCR4 stimulates macropinocytosis: implications for cellular uptake of arginine-rich cell-penetrating peptides and HIV.Chemistry & biology, 19 11
J. Mehta, Nobuhito Sanada, Changping Hu, Jiawei Chen, A. Dandapat, F. Sugawara, Hiroo Satoh, Kazuhiko Inoue, Y. Kawase, K. Jishage, Hiroshi Suzuki, M. Takeya, L. Schnackenberg, R. Beger, P. Hermonat, M. Thomas, T. Sawamura (2007)
Deletion of LOX-1 Reduces Atherogenesis in LDLR Knockout Mice Fed High Cholesterol DietCirculation Research, 100
K. Wallner, Chen Li, P. Shah, M. Fishbein, J. Forrester, S. Kaul, B. Sharifi (1999)
Tenascin-C is expressed in macrophage-rich human coronary atherosclerotic plaque.Circulation, 99 10
F. Pérez-López, Luis Larrad-Mur, Amanda Kallen, P. Chedraui, H. Taylor (2010)
Review: Gender Differences in Cardiovascular Disease: Hormonal and Biochemical InfluencesReproductive Sciences, 17
C. Amălinei, I. Căruntu, R. Balan (2007)
Biology of metalloproteinases.Romanian journal of morphology and embryology = Revue roumaine de morphologie et embryologie, 48 4
J. Mehta, Jiawei Chen, P. Hermonat, F. Romeo, G. Novelli (2006)
Lectin-like, oxidized low-density lipoprotein receptor-1 (LOX-1): a critical player in the development of atherosclerosis and related disorders.Cardiovascular research, 69 1
MA Shibata, E Shibata, S Fujioka, M Harada-Shiba (2015)
Atherosclerosis in apolipoprotein E-knockout mice as a model of human diseaseAustin J Cardiovasc Dis Atherosclerosis, 2
D. Wuttge, Xinghua Zhou, Y. Sheikine, D. Wågsäter, V. Stemme, U. Hedin, S. Stemme, G. Hansson, A. Sirsjö (2004)
CXCL16/SR-PSOX Is an Interferon-&ggr;–Regulated Chemokine and Scavenger Receptor Expressed in Atherosclerotic LesionsArteriosclerosis, Thrombosis, and Vascular Biology: Journal of the American Heart Association, 24
R. Tangirala, E. Rubin, W. Palinski (1995)
Quantitation of atherosclerosis in murine models: correlation between lesions in the aortic origin and in the entire aorta, and differences in the extent of lesions between sexes in LDL receptor-deficient and apolipoprotein E-deficient mice.Journal of lipid research, 36 11
R. Coleman, T. Hayek, S. Keidar, M. Aviram (2006)
A mouse model for human atherosclerosis: long-term histopathological study of lesion development in the aortic arch of apolipoprotein E-deficient (E0) mice.Acta histochemica, 108 6
M. Shibata, E. Shibata, S. Fujioka, M. Harada‐Shiba (2015)
Apolipoprotein E-knockout Mice as a Lifestyle-related Disease Model of Atherosclerosis and Non-alcoholic Fatty Liver DiseaseInternational Journal of Laboratory Medicine, 2015
E. Galkina, B. Harry, A. Ludwig, E. Liehn, J. Sanders, Anthony Bruce, C. Weber, K. Ley (2007)
CXCR6 Promotes Atherosclerosis by Supporting T-Cell Homing, Interferon-&ggr; Production, and Macrophage Accumulation in the Aortic WallCirculation, 116
Kazuhiko Inoue, Yuji Arai, H. Kurihara, T. Kita, T. Sawamura (2005)
Overexpression of Lectin-Like Oxidized Low-Density Lipoprotein Receptor-1 Induces Intramyocardial Vasculopathy in Apolipoprotein E–Null MiceCirculation Research, 97
Y. Vengrenyuk, S. Carlier, S. Xanthos, L. Cardoso, P. Ganatos, R. Virmani, S. Einav, L. Gilchrist, S. Weinbaum (2006)
A hypothesis for vulnerable plaque rupture due to stress-induced debonding around cellular microcalcifications in thin fibrous capsProceedings of the National Academy of Sciences, 103
M. Rouis, C. Adamy, N. Duverger, P. Lesnik, P. Horellou, M. Moreau, F. Emmanuel, J. Caillaud, P. Laplaud, C. Dachet, M. Chapman (1999)
Adenovirus-mediated overexpression of tissue inhibitor of metalloproteinase-1 reduces atherosclerotic lesions in apolipoprotein E-deficient mice.Circulation, 100 5
K. Rychli, B. Richter, P. Hohensinner, Kariem Ali, S. Neuhold, G. Zorn, R. Berger, D. Mörtl, K. Huber, R. Pacher, J. Wojta, A. Niessner, M. Hülsmann (2011)
Hepatocyte growth factor is a strong predictor of mortality in patients with advanced heart failureHeart, 97
A. Plump, Jonathan Smith, T. Hayek, K. Aalto-setälä, A. Walsh, J. Verstuyft, E. Rubin, J. Breslow (1992)
Severe hypercholesterolemia and atherosclerosis in apolipoprotein E-deficient mice created by homologous recombination in ES cellsCell, 71
Ian Loftus, A. Naylor, S. Goodall, M. Crowther, Louise Jones, P. Bell, Matthew Thompson (2000)
Increased matrix metalloproteinase-9 activity in unstable carotid plaques. A potential role in acute plaque disruption.Stroke, 31 1
K. Olczyk, M. Dróżdż, B. Piwowarczyk (1981)
Activity of selected enzymes in arterial wall and blood serum of rats with experimental atherosclerosis.Acta biologica et medica Germanica, 40 6
K. Livak, Thomas Schmittgen (2001)
Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) Method.Methods, 25 4
E. Vorst, Y. Döring, C. Weber (2015)
Chemokines and their receptors in AtherosclerosisJournal of Molecular Medicine (Berlin, Germany), 93
J. Sundström, R. Vasan (2006)
Circulating biomarkers of extracellular matrix remodeling and risk of atherosclerotic eventsCurrent Opinion in Lipidology, 17
M. Denis, J. Marcinkiewicz, A. Zaid, D. Gauthier, Steve Poirier, C. Lazure, N. Seidah, A. Prat (2012)
Gene Inactivation of PCSK9 Reduces Atherosclerosis in Mice
R. Elhage, J. Arnal, M. Pieraggi, N. Duverger, C. Fiévet, J. Faye, F. Bayard (1997)
17β-Estradiol Prevents Fatty Streak Formation in Apolipoprotein E–Deficient MiceArteriosclerosis, Thrombosis, and Vascular Biology, 17
K. Ono, A. Matsumori, T. Shioi, Yutaka Furukawa, S. Sasayama (1997)
Enhanced expression of hepatocyte growth factor/c-Met by myocardial ischemia and reperfusion in a rat model.Circulation, 95 11
J. Piedrahita, Sunny Zhang, J. Hagaman, P. Oliver, N. Maeda (1992)
Generation of mice carrying a mutant apolipoprotein E gene inactivated by gene targeting in embryonic stem cells.Proceedings of the National Academy of Sciences of the United States of America, 89
A. Luttun, E. Lutgens, Ann Manderveld, K. Maris, D. Collen, P. Carmeliet, L. Moons (2004)
Loss of Matrix Metalloproteinase-9 or Matrix Metalloproteinase-12 Protects Apolipoprotein E–Deficient Mice Against Atherosclerotic Media Destruction but Differentially Affects Plaque GrowthCirculation: Journal of the American Heart Association, 109
A. Aslanian, I. Charo (2006)
Targeted Disruption of the Scavenger Receptor and Chemokine CXCL16 Accelerates AtherosclerosisCirculation, 114
Miller Bf, Keyes Fp, Curreri Pw (1966)
Beta-glucuronidase activity in serum increased by coronary-artery atherosclerosis.Science, 152
Jonas Hansson, R. Vasan, J. Ärnlöv, E. Ingelsson, L. Lind, A. Larsson, K. Michaëlsson, J. Sundström (2011)
Biomarkers of Extracellular Matrix Metabolism (MMP-9 and TIMP-1) and Risk of Stroke, Myocardial Infarction, and Cause-Specific Mortality: Cohort StudyPLoS ONE, 6
M. Denis, J. Marcinkiewicz, A. Zaid, D. Gauthier, Steve Poirier, C. Lazure, N. Seidah, A. Prat (2012)
Gene Inactivation of Proprotein Convertase Subtilisin/Kexin Type 9 Reduces Atherosclerosis in MiceCirculation, 125
S. Meyrelles, V. Peotta, Thiago Pereira, E. Vasquez (2011)
Endothelial Dysfunction in the Apolipoprotein E-deficient Mouse: insights into the influence of diet, gender and agingLipids in Health and Disease, 10
J. Silence, D. Collen, H. Lijnen (2002)
Reduced Atherosclerotic Plaque but Enhanced Aneurysm Formation in Mice With Inactivation of the Tissue Inhibitor of Metalloproteinase-1 (TIMP-1) GeneCirculation Research: Journal of the American Heart Association, 90
D. Chistiakov, I. Sobenin, A. Orekhov (2013)
Vascular Extracellular Matrix in AtherosclerosisCardiology in Review, 21
D. Smith, X. Tan, O. Tawfik, G. Milne, D. Stechschulte, K. Dileepan (2010)
Increased aortic atherosclerotic plaque development in female apolipoprotein E-null mice is associated with elevated thromboxane A2 and decreased prostacyclin production.Journal of physiology and pharmacology : an official journal of the Polish Physiological Society, 61 3
M. Minami, N. Kume, T. Shimaoka, H. Kataoka, K. Hayashida, Y. Akiyama, Izumi Nagata, K. Ando, M. Nobuyoshi, M. Hanyuu, M. Komeda, S. Yonehara, T. Kita (2001)
Expression of SR-PSOX, a Novel Cell-Surface Scavenger Receptor for Phosphatidylserine and Oxidized LDL in Human Atherosclerotic LesionsArteriosclerosis, Thrombosis, and Vascular Biology: Journal of the American Heart Association, 21
J. Orbe, L. Fernandez, J. Rodríguez, G. Rábago, M. Belzunce, A. Monasterio, C. Roncal, J. Paramo (2003)
Different expression of MMPs/TIMP-1 in human atherosclerotic lesions. Relation to plaque features and vascular bed.Atherosclerosis, 170 2
E Vorst, Y Doring, C Weber (2015)
ChemokinesArterioscler Thromb Vasc Biol, 35
H. Kataoka, N. Kume, S. Miyamoto, M. Minami, H. Moriwaki, T. Murase, T. Sawamura, Tomoh Masaki, N. Hashimoto, T. Kita (1999)
Expression of lectinlike oxidized low-density lipoprotein receptor-1 in human atherosclerotic lesions.Circulation, 99 24
Takako Sugisawa, T. Okamura, H. Makino, Makoto Watanabe, I. Kishimoto, Y. Miyamoto, N. Iwamoto, A. Yamamoto, S. Yokoyama, M. Harada‐Shiba (2012)
Defining patients at extremely high risk for coronary artery disease in heterozygous familial hypercholesterolemia.Journal of atherosclerosis and thrombosis, 19 4
M. Ogura, M. Hori, M. Harada‐Shiba (2016)
Association Between Cholesterol Efflux Capacity and Atherosclerotic Cardiovascular Disease in Patients With Familial HypercholesterolemiaArteriosclerosis, Thrombosis, and Vascular Biology, 36
C. White (2014)
Carotid artery stenting.Journal of the American College of Cardiology, 64 7
Tsuyoshi Yamamoto, S. Obika, Moeka Nakatani, Hidenori Yasuhara, Fumito Wada, E. Shibata, M. Shibata, M. Harada‐Shiba (2014)
Locked nucleic acid antisense inhibitor targeting apolipoprotein C-III efficiently and preferentially removes triglyceride from large very low-density lipoprotein particles in murine plasma.European journal of pharmacology, 723
J. Li, K. Ley (2015)
Lymphocyte Migration Into Atherosclerotic PlaqueArteriosclerosis, Thrombosis, and Vascular Biology, 35
B. Paigen, A. Morrow, Catherine Brandon, D. Mitchell, P. Holmes (1985)
Variation in susceptibility to atherosclerosis among inbred strains of mice.Atherosclerosis, 57 1
N. Kume, T. Kita (2001)
Lectin-like oxidized low-density lipoprotein receptor-1 (LOX-1) in atherogenesis.Trends in cardiovascular medicine, 11 1
M. Harada‐Shiba, H. Arai, S. Oikawa, T. Ohta, T. Okada, T. Okamura, A. Nohara, H. Bujo, K. Yokote, A. Wakatsuki, S. Ishibashi, S. Yamashita (2012)
Guidelines for the management of familial hypercholesterolemia.Journal of atherosclerosis and thrombosis, 19 12
CJ Slager, J. Wentzel, F. Gijsen, J.C.H. Schuurbiers, A. Wal, A. Steen, P. Serruys (2005)
The role of shear stress in the generation of rupture-prone vulnerable plaquesNature Clinical Practice Cardiovascular Medicine, 2
Tsuyoshi Yamamoto, M. Harada‐Shiba, Moeka Nakatani, Shunsuke Wada, Hidenori Yasuhara, K. Narukawa, Kiyomi Sasaki, M. Shibata, H. Torigoe, T. Yamaoka, T. Imanishi, S. Obika (2012)
Cholesterol-lowering Action of BNA-based Antisense Oligonucleotides Targeting PCSK9 in Atherogenic Diet-induced Hypercholesterolemic MiceMolecular Therapy. Nucleic Acids, 1
The establishment of consistent and reliable methods for the analysis of atherosclerosis molecular pathways and for testing the efficiency of new therapeutics is of utmost importance. Here, we fed ApoE-knockout (KO) mice with high-fat diet to for 16 weeks to induce atherosclerosis. Atherosclerotic lesions in mice were methodically investigated using pathologic analyses and molecular biology tools. These lesions were histopathologically classified into three categories: early, progressive, and combined lesions. Immunohistochemical analyses showed that both F4/80 (macrophage marker) and tenascin-C are expressed in these lesions. Real-time PCR analysis conducted using formalin-fixed paraffin-embedded tissues with atherosclerotic lesions demonstrated an increase in the levels of many inflammatory chemokines, including Cxcl16, while antibody arrays performed using frozen atherosclerotic tissue samples showed elevated TIMP-1 expression. Subsequent immunohistochemical analyses showed that the expression of CXCL16, TIMP-1, MMP-9, MMP-8, and LOX-1 is localized in the atherosclerotic lesions. We confirmed that the expression of these proteins is localized to atherosclerotic lesion, which suggests their roles in the development of the lesions in ApoE-KO mice. Therefore, this mouse model represents an appropriate tool for elucidating molecular mechanisms underlying the development of atherosclerosis, and a model for the evaluation of therapeutic efficiency of novel drugs.
Medical Molecular Morphology – Springer Journals
Published: Feb 28, 2017
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