Partial deletion of stem-loop 2 in the 3′ untranslated region of foot-and-mouth disease virus identifies a region that is dispensable for virus replication

Partial deletion of stem-loop 2 in the 3′ untranslated region of foot-and-mouth disease virus... The 3′ untranslated region (3′ UTR) of the foot-and-mouth disease virus (FMDV) genome plays an essential role in virus replication, but the properties of the 3′ UTR are not completely defined. In order to determine the role of different regions of the 3′ UTR in FMDV replication, we conducted site-directed mutagenesis of the 3′ UTR of FMDV serotype O IND R2/1975 using a cDNA clone. Through independent serial deletions in various regions of the 3′ UTR, we demonstrated that deletion of nucleotides between the stem-loop (SL) structures and in the beginning and the end regions of the SL2 structure could be lethal for FMDV replication. However, a block deletion of 20 nucleotides (nt 60 to 79) in the middle of SL2 did not affect the viability of FMDV in cultured cells. Characterisation of the deletion mutant virus (OR2/1975-Δ3′UTR 60–79) revealed no significant difference in growth kinetics or RNA replication ability compared to the parental virus. However, the mutant virus produced slightly larger plaques when compared to the parental virus. This is the first description of a dispensable 20-nucleotide region in SL2 of the FMDV 3′ UTR. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Archives of Virology Springer Journals

Partial deletion of stem-loop 2 in the 3′ untranslated region of foot-and-mouth disease virus identifies a region that is dispensable for virus replication

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Publisher
Springer Journals
Copyright
Copyright © 2016 by Springer-Verlag Wien
Subject
Biomedicine; Virology; Medical Microbiology; Infectious Diseases
ISSN
0304-8608
eISSN
1432-8798
D.O.I.
10.1007/s00705-016-2909-5
Publisher site
See Article on Publisher Site

Abstract

The 3′ untranslated region (3′ UTR) of the foot-and-mouth disease virus (FMDV) genome plays an essential role in virus replication, but the properties of the 3′ UTR are not completely defined. In order to determine the role of different regions of the 3′ UTR in FMDV replication, we conducted site-directed mutagenesis of the 3′ UTR of FMDV serotype O IND R2/1975 using a cDNA clone. Through independent serial deletions in various regions of the 3′ UTR, we demonstrated that deletion of nucleotides between the stem-loop (SL) structures and in the beginning and the end regions of the SL2 structure could be lethal for FMDV replication. However, a block deletion of 20 nucleotides (nt 60 to 79) in the middle of SL2 did not affect the viability of FMDV in cultured cells. Characterisation of the deletion mutant virus (OR2/1975-Δ3′UTR 60–79) revealed no significant difference in growth kinetics or RNA replication ability compared to the parental virus. However, the mutant virus produced slightly larger plaques when compared to the parental virus. This is the first description of a dispensable 20-nucleotide region in SL2 of the FMDV 3′ UTR.

Journal

Archives of VirologySpringer Journals

Published: May 27, 2016

References

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