Get 20M+ Full-Text Papers For Less Than $1.50/day. Start a 14-Day Trial for You or Your Team.

Learn More →

Pairwise and higher-order genetic interactions during the evolution of a tRNA

Pairwise and higher-order genetic interactions during the evolution of a tRNA A central question in genetics and evolution is the extent to which the outcomes of mutations change depending on the genetic context in which they occur 1–3 . Pairwise interactions between mutations have been systematically mapped within 4–18 and between 19 genes, and have been shown to contribute substantially to phenotypic variation among individuals 20 . However, the extent to which genetic interactions themselves are stable or dynamic across genotypes is unclear 21, 22 . Here we quantify more than 45,000 genetic interactions between the same 87 pairs of mutations across more than 500 closely related genotypes of a yeast tRNA. Notably, all pairs of mutations interacted in at least 9% of genetic backgrounds and all pairs switched from interacting positively to interacting negatively in different genotypes (false discovery rate < 0.1). Higher-order interactions are also abundant and dynamic across genotypes. The epistasis in this tRNA means that all individual mutations switch from detrimental to beneficial, even in closely related genotypes. As a consequence, accurate genetic prediction requires mutation effects to be measured across different genetic backgrounds and the use of  higher-order epistatic terms. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Nature Springer Journals

Pairwise and higher-order genetic interactions during the evolution of a tRNA

Nature , Volume 558 (7708) – May 30, 2018

Loading next page...
 
/lp/springer_journal/pairwise-and-higher-order-genetic-interactions-during-the-evolution-of-3XIXHx4mZE

References (43)

Publisher
Springer Journals
Copyright
Copyright © 2018 by Macmillan Publishers Ltd., part of Springer Nature
Subject
Science, Humanities and Social Sciences, multidisciplinary; Science, Humanities and Social Sciences, multidisciplinary; Science, multidisciplinary
ISSN
0028-0836
eISSN
1476-4687
DOI
10.1038/s41586-018-0170-7
pmid
29849145
Publisher site
See Article on Publisher Site

Abstract

A central question in genetics and evolution is the extent to which the outcomes of mutations change depending on the genetic context in which they occur 1–3 . Pairwise interactions between mutations have been systematically mapped within 4–18 and between 19 genes, and have been shown to contribute substantially to phenotypic variation among individuals 20 . However, the extent to which genetic interactions themselves are stable or dynamic across genotypes is unclear 21, 22 . Here we quantify more than 45,000 genetic interactions between the same 87 pairs of mutations across more than 500 closely related genotypes of a yeast tRNA. Notably, all pairs of mutations interacted in at least 9% of genetic backgrounds and all pairs switched from interacting positively to interacting negatively in different genotypes (false discovery rate < 0.1). Higher-order interactions are also abundant and dynamic across genotypes. The epistasis in this tRNA means that all individual mutations switch from detrimental to beneficial, even in closely related genotypes. As a consequence, accurate genetic prediction requires mutation effects to be measured across different genetic backgrounds and the use of  higher-order epistatic terms.

Journal

NatureSpringer Journals

Published: May 30, 2018

There are no references for this article.