Wheat (Triticum aestivumL.) root mitochondria were used to investigate the effect of solute concentration in the medium on the rates of succinate, malate, α-ketoglutarate, and glutamate oxidation; the corresponding values of the apparent Michaelis constant (K M); and the changes in the organelle volumes. The oxidation rates and the K M values for all substrates were lower in the 0.5 M sucrose solution than in the 0.3 M solution. Under high osmotic concentration, mitochondria did not shrink. On the contrary, in the absence of the substrate, mitochondria swelled more in the 0.5 M sucrose solution than in the 0.3 M solution. This effect was absent when the substrate was added. The authors conclude that the decrease in the oxidation rate imposed by sucrose was not related to the hindered influx of substrates caused by matrix contraction. Rather, the osmotic effect of sucrose is related to the changes in the structure of mitochondrial enzyme complexes.
Russian Journal of Plant Physiology – Springer Journals
Published: Oct 10, 2004
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