Mammalian Genome 8, 770–772 (1997). © Springer-Verlag New York Inc. 1997 Ovine growth hormone gene duplication—structural and evolutionary implications Rachel Ofir, Elisha Gootwine Institute of Animal Science, Agricultural Research Organization, The Volcani Center, Bet Dagan 50250, Israel Received: 20 January 1997 / Accepted: 2 June 1997 Table 1. Primers used to amplify different regions of the ovine GH gene The growth hormone (GH) gene belongs to a gene family that also includes the chorionic somatomammotropin (placental lactogen) Primer gene, the prolactin gene, and several prolactin-like genes, all No. Position Orientation Sequence (58 to 38) evolved through series of gene duplications (Ohta 1993; Wallis 1 145 58 38 TTATCCATTAGCACAGGCTGCCAGTG 1993, 1994, 1996). The ovine GH gene is about 1.8 kb long and 2 144 58 38 ATTATCCATTAGCACAGGCTGCCA contains five exons and four introns (Byrne et al. 1987; Orian et al. 3 453 38 58 TCAAACTTGGCCAAATGTCGGGTG 1988). Previous studies (Valinsky et al. 1990; Gootwine et al. 4 466 58 38 GGCCAAGTTTGAAATGTTCTCAG 1993) have shown that gene duplication occurred at the ovine GH 5 587 58 38 ACCTCCCTGCTCCTGGCTTTCA 6 706 58 38 GCATCAACTGGCTACTGACACC locus, and two alleles are found: the GH1 allele with a single GH 7 752 38 58 CTGGGGAGCTTACAAACTCTTT copy,
Mammalian Genome – Springer Journals
Published: Oct 1, 1997
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