Overexpression of UDP-glucosyltransferase 71C5 increases glucose tolerance in Arabidopsis thaliana

Overexpression of UDP-glucosyltransferase 71C5 increases glucose tolerance in Arabidopsis thaliana Glucosyltransferases (GTs) catalyze transfer of sugar moieties to a wide range of acceptor molecules, which could be proteins, lipids, or low-molecular-weight lipophilic acceptors. As a subfamily of GTs, uridine diphosphate (UDP)-glucosyltransferases (UGTs), which function is glycosylation of low-molecularweight lipophilic acceptors, has drawn increasing attention. In this study, it was found that UGT71C5 expression in Arabidopsis thaliana treated by 4% glucose could be induced to approximately 250 and 180% of that in the wild type (WT) at both transcriptional and translational levels. Three independent lines overexpressing UGT71C5 were obtained and quantitatively analyzed. It was shown that the average root length of 35S::UGT71C5 transgenic lines was doubled as compared with WT on MS supplemented with 4% glucose. To our knowledge, in higher plants glucose acts as both nutrient and regulatory molecule, which controls many aspects of physiology and development, and the high levels of glucose could arrest glucose-mediated root elongation in plants. Our work suggests that overexpression of UGT71C5 could increase glucose tolerance of A. thaliana. This study could provide insights into the function of UGT71C5 in plants. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Russian Journal of Plant Physiology Springer Journals

Overexpression of UDP-glucosyltransferase 71C5 increases glucose tolerance in Arabidopsis thaliana

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Publisher
SP MAIK Nauka/Interperiodica
Copyright
Copyright © 2010 by Pleiades Publishing, Ltd.
Subject
Life Sciences; Plant Sciences ; Plant Physiology
ISSN
1021-4437
eISSN
1608-3407
D.O.I.
10.1134/S1021443710060178
Publisher site
See Article on Publisher Site

Abstract

Glucosyltransferases (GTs) catalyze transfer of sugar moieties to a wide range of acceptor molecules, which could be proteins, lipids, or low-molecular-weight lipophilic acceptors. As a subfamily of GTs, uridine diphosphate (UDP)-glucosyltransferases (UGTs), which function is glycosylation of low-molecularweight lipophilic acceptors, has drawn increasing attention. In this study, it was found that UGT71C5 expression in Arabidopsis thaliana treated by 4% glucose could be induced to approximately 250 and 180% of that in the wild type (WT) at both transcriptional and translational levels. Three independent lines overexpressing UGT71C5 were obtained and quantitatively analyzed. It was shown that the average root length of 35S::UGT71C5 transgenic lines was doubled as compared with WT on MS supplemented with 4% glucose. To our knowledge, in higher plants glucose acts as both nutrient and regulatory molecule, which controls many aspects of physiology and development, and the high levels of glucose could arrest glucose-mediated root elongation in plants. Our work suggests that overexpression of UGT71C5 could increase glucose tolerance of A. thaliana. This study could provide insights into the function of UGT71C5 in plants.

Journal

Russian Journal of Plant PhysiologySpringer Journals

Published: Oct 31, 2010

References

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