J. For. Res. https://doi.org/10.1007/s11676-018-0709-3 ORIGINAL PAPER Overexpression of the LoMYB29 gene of Larix olgensis contributes to the regulation of proanthocyanidin biosynthesis in Arabidopsis thaliana 1 2 1 • • Dandan Li Xiaoqing Hu Chenghao Li Received: 29 November 2017 / Accepted: 6 February 2018 Northeast Forestry University and Springer-Verlag GmbH Germany, part of Springer Nature 2018 Abstract Proanthocyanidins (PAs) are the most broadly LDOX, and ANR (PA branch gene), were also activated in distributed secondary metabolites that play important roles transgenic plants overexpressing LoMYB29. The results in various aspects of plant development and response to indicate that LoMYB29 plays a positive role in the regu- biotic and abiotic stresses. In this study, we cloned a R2R3 lation of PA biosynthesis by activating the expression of MYB gene LoMYB29, which has a full-length coding PA biosynthetic genes. sequence of 921 bp identiﬁed in Larix olgensis. Quantita- tive real-time reverse transcription polymerase chain Keywords Olga Bay larch MYB transcription factors reaction analysis indicates that LoMYB29 is expressed TT2 Proanthocyanidin under mechanical wounding, high light intensity, and NaCl, PEG6000, Methyl Jasmonate, and abscisic acid treatments. Subcellular localization analysis and yeast two- Introduction hybrid assay localized LoMYB29 to the nucleus, acting
Journal of Forestry Research – Springer Journals
Published: May 28, 2018
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