Access the full text.
Sign up today, get DeepDyve free for 14 days.
F. Katagiri, E. Lam, N. Chua (1989)
Two tobacco DNA-binding proteins with homology to the nuclear factor CREBNature, 340
N. Vetten, R. Ferl (1994)
Transcriptional regulation of environmentally inducible genes in plants by an evolutionary conserved family of G-box binding factors.The International journal of biochemistry, 26 9
M. Guiltinan, W. Marcotte, R. Quatrano (1990)
A plant leucine zipper protein that recognizes an abscisic acid response element.Science, 250 4978
G. Cassab, J. Varner (1987)
Immunocytolocalization of extensin in developing soybean seed coats by immunogold-silver staining and by tissue printing on nitrocellulose paperThe Journal of Cell Biology, 105
A. Menkens, U. Schindler, A. Cashmore (1995)
The G-box: a ubiquitous regulatory DNA element in plants bound by the GBF family of bZIP proteins.Trends in biochemical sciences, 20 12
J Sambrook, EF Fritsch, T Maniatis (1989)
Molecular Cloning: A Laboratory Manual
(1989)
Molecular Cloning: A Laboratory Manual, 2nd ed
R. Quatrano, W. Marcotte, M. Guiltinan, D. Verma (1993)
Regulation of gene expression by abscisic acid.
L. Kuras, R. Barbey, Dominique Thomas (1997)
Assembly of a bZIP–bHLH transcription activation complex: formation of the yeast Cbf1–Met4–Met28 complex is regulated through Met28 stimulation of Cbf1 DNA bindingThe EMBO Journal, 16
A. Lloyd, N. Yancheva, B. Wasylyk (1991)
Transformation suppressor activity of a Jun transcription factor lacking its activation domainNature, 352
D. Bartholomew, G. Bartley, P. Scolnik (1991)
Abscisic Acid Control of rbcS and cab Transcription in Tomato Leaves.Plant physiology, 96 1
W. Marcotte, S. Russell, R. Quatrano (1989)
Abscisic acid-responsive sequences from the em gene of wheat.The Plant cell, 1
P. Maliga, Agnes Sz.-Breznovits, L. Marton (1973)
Streptomycin-resistant plants from callus culture of haploid tobacco.Nature: New biology, 244 131
Q. Shen, T. Ho (1995)
Functional dissection of an abscisic acid (ABA)-inducible gene reveals two independent ABA-responsive complexes each containing a G-box and a novel cis-acting element.The Plant cell, 7 3
Barbara Mazur, C. Chui (1985)
Sequence of a genomic DNA clone for the small subunit of ribulose bis-phosphate carboxylase-oxygenase from tobacco.Nucleic acids research, 13 7
The bZIP protein mEMBP - 1 regulates transcription via an O 2 box sequence
M. Rieping, M. Fritz, S. Prat, C. Gatz (1994)
A dominant negative mutant of PG13 suppresses transcription from a cauliflower mosaic virus 35S truncated promoter in transgenic tobacco plants.The Plant cell, 6
R. Foster, T. Izawa, N. Chua (1994)
Plant bZIP proteins gather at ACGT elementsThe FASEB Journal, 8
F. Katagiri, N. Chua (1992)
Plant transcription factors: present knowledge and future challenges.Trends in genetics : TIG, 8 1
O. Faktor, G. Loake, R. Dixon, C. Lamb (1997)
The G‐box and H‐box in a 39 bp region of a French bean chalcone synthase promoter constitute a tissue‐specific regulatory elementPlant Journal, 11
R. Donald, A. Cashmore (1990)
Mutation of either G box or I box sequences profoundly affects expression from the Arabidopsis rbcS‐1A promoter.The EMBO Journal, 9
X. Niu, M. Guiltinan (1994)
DNA binding specificity of the wheat bZIP protein EmBP-1.Nucleic acids research, 22 23
B. Genty, J. Briantais, N. Baker (1989)
The relationship between the quantum yield of photosynthetic electron transport and quenching of chlorophyll fluorescenceBiochimica et Biophysica Acta, 990
G. Giuliano, E. Pichersky, V. Malik, M. Timko, P. Scolnik, A. Cashmore (1988)
An evolutionarily conserved protein binding sequence upstream of a plant light-regulated gene.Proceedings of the National Academy of Sciences of the United States of America, 85 19
I. Hope, K. Struhl (1986)
Functional dissection of a eukaryotic transcriptional activator protein, GCN4 of YeastCell, 46
RB Horsch, J Fry, N Hoffmann, J Neidermeyer, SG Rogers, RT Fraley (1988)
Plant Molecular Biology Manual
RS Quatrano, WR Marcotte, M Guiltinan (1993)
Control of Plant Gene Expression
G. Sessa, Y. Meller, R. Fluhr (1995)
A GCC element and a G-box motif participate in ethylene-induced expression of the PRB-1b genePlant Molecular Biology, 28
M. Feldbrügge, Markus Sprenger, K. Hahlbrock, B. Weisshaar (1997)
PcMYB1, a novel plant protein containing a DNA-binding domain with one MYB repeat, interacts in vivo with a light-regulatory promoter unit.The Plant journal : for cell and molecular biology, 11 5
B. Orozco, W. Ogren (1993)
Localization of light-inducible and tissue-specific regions of the spinach ribulose bisphosphate carboxylase/oxygenase (rubisco) activase promoter in transgenic tobacco plantsPlant Molecular Biology, 23
E. Krebbers, J. Seurinck, L. Herdies, A. Cashmore, M. Timko (1988)
Four genes in two diverged subfamilies encode the ribulose-1,5-bisphosphate carboxylase small subunit polypeptides of Arabidopsis thalianaPlant Molecular Biology, 11
O. Burk, S. Mink, M. Ringwald, K. Klempnauer (1993)
Synergistic activation of the chicken mim‐1 gene by v‐myb and C/EBP transcription factors.The EMBO Journal, 12
S. Ha, G. An (1988)
Identification of upstream regulatory elements involved in the developmental expression of the Arabidopsis thaliana cab1 gene.Proceedings of the National Academy of Sciences of the United States of America, 85 21
R. Horsch, J. Fry, N. Hoffmann, Jeanne Neidermeyer, S. Rogers, R. Fraley (1989)
Leaf disc transformation
(1986)
A simple method for extraction of RNA from maize tissue
W. McKendree, A. Paul, A. Delisle, R. Ferl (1990)
In vivo and in vitro characterization of protein interactions with the dyad G-box of the Arabidopsis Adh gene.The Plant cell, 2
M. Guiltinan, L. Mchenry (1995)
Epitope tagging for the detection of fusion protein expression in transgenic plants.Methods in cell biology, 49
M. Olive, S. Williams, Christine Dezan, P. Johnson, C. Vinson (1996)
Design of a C/EBP-specific, Dominant-negative bZIP Protein with Both Inhibitory and Gain-of-function Properties *The Journal of Biological Chemistry, 271
E. Unger, R. Parsons, R. Schmidt, B. Bowen, B. Roth (1993)
Dominant Negative Mutants of Opaque2 Suppress Transactivation of a 22-kD Zein Promoter by Opaque2 in Maize Endosperm Cells.The Plant cell, 5
G. Evan, G. Lewis, G. Ramsay, J. Bishop (1985)
Isolation of monoclonal antibodies specific for human c-myc proto-oncogene productMolecular and Cellular Biology, 5
K. Devos, M. Atkinson, C. Chinoy, M. Guiltinan, M. Guiltinan, Ralph Quatrano, Ralph Quatrano, M. Gale (1991)
Chromosomal location and variability in wheat, barley and rye of a wheat gene encoding a bZIP protein (EmBP-1)Theoretical and Applied Genetics, 82
T. Tabata, H. Takase, S. Takayama, K. Mikami, A. Nakatsuka, T. Kawata, T. Nakayama, M. Iwabuchi (1989)
A protein that binds to a cis-acting element of wheat histone genes has a leucine zipper motif.Science, 245 4921
B. Karunanandaa, S. Huang, T. Kao (1994)
Carbohydrate moiety of the Petunia inflata S3 protein is not required for self-incompatibility interactions between pollen and pistil.The Plant cell, 6
As a first step toward elucidating the in vivo function of plant bZIP proteins and their related G-box cis elements, we have introduced a dominant negative inhibitor of G-box-dependent transcriptional activation into tobacco plants by transforming them with a truncated EmBP-1 gene (δEmBP) containing the DNA binding and dimerization domains under the control of the CaMV 35S promoter. Five independent lines of transgenic plants expressing δEmBP were identified, as demonstrated by immunodetection of the transgenic protein in leaf extracts, and the ability of the protein to bind a target G-box DNA sequence. The transgenic plants exhibited an abnormal phenotype characterized by interveinal chlorosis, growth inhibition and weakening of stems and petioles, the severity of which positively correlated with δEmBP expression and G-box DNA binding capability. Furthermore, development of chlorosis and growth inhibition was dependent on growth irradiance. Low light promoted the development of interveinal chlorosis and growth inhibition in the transgenic plants, whereas high light conditions led to near-complete amelioration of the abnormal phenotype. Transgenic plants under both light regimes showed signs of impaired stem and petiole function which was not observed in wild-type tobacco. RbcS gene expression was not significantly altered by δEmBP expression, suggesting that down-regulation of this gene was not responsible for the altered phenotype. The results suggest that G-box elements specific for the EmBP-1 class of bZIP proteins have an important developmental function in vegetative plant tissues, and that the trans-dominant negative mutant approach is a useful tool for continued in vivo functional analysis of bZIP transcription factors and their corresponding cis elements in plants.
Plant Molecular Biology – Springer Journals
Published: Oct 6, 2004
Read and print from thousands of top scholarly journals.
Already have an account? Log in
Bookmark this article. You can see your Bookmarks on your DeepDyve Library.
To save an article, log in first, or sign up for a DeepDyve account if you don’t already have one.
Copy and paste the desired citation format or use the link below to download a file formatted for EndNote
Access the full text.
Sign up today, get DeepDyve free for 14 days.
All DeepDyve websites use cookies to improve your online experience. They were placed on your computer when you launched this website. You can change your cookie settings through your browser.