Plant Mol Biol (2017) 93:313–326 DOI 10.1007/s11103-016-0563-7 Over-expression of miR158 causes pollen abortion in Brassica campestris ssp. chinensis 1 1 1 1 1 Zhiming Ma · Jianxia Jiang · Ziwei Hu · Tianqi Lyu · Yang Yang · 2 1 Jingjing Jiang · Jiashu Cao Received: 22 March 2016 / Accepted: 12 November 2016 / Published online: 1 December 2016 © Springer Science+Business Media Dordrecht 2016 Abstract and their target gene bra027656, which encodes a pentatri- Key message We identified and cloned the two precur - copeptide repeat (PPR) containing protein, were identi- sors of miR158 and its target gene in Brassica campes- fied. Then, qRT-PCR analysis and GUS-reporter system tris ssp. chinensis, which both had high relative expres- revealed that both bra-miR158 and its target gene had sion in the inflorescences. Further study revealed that relatively high expression levels in the inflorescences. Fur - over-expression of miR158 caused reduced pollen var- ther study revealed that over-expression of miR158 caused bility, which was caused by the degradation of pollen reduced pollen varbility and pollen germination ratio, and contents from the binucleate microspore stage. These the degradation of pollen contents from the binucleate results first suggest the role of miR158 in pollen devel- microspore stage was also found
Plant Molecular Biology – Springer Journals
Published: Dec 1, 2016
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