The effect of over-expression of AtDREB1A gene in chrysanthemums on heat stress tolerance was investigated. Transgenic plants with 35S:AtDREB1A construct (henceforth, 35S plants) and wild type (WT) plants were exposed to 45°C as a heat stress treatment. When heat-treated for 36 h and followed by a 3-week recovery, approximately 70% of the 35S plants, but less than 20% of the WT plants were survived. Leaf electrolyte leakage was significantly lower in 35S plants than in WT plants. cDNA macroarray analysis showed that 55 of 74 DREB1A regulon members were up-regulated under heat stress in WT plants. In comparison with WT plants, 35S plants displayed greatly enhanced expression of the genes including signal transduction, transcription and HSP70 in the early time, and the genes including photosynthesis and metabolism in the late time of the heat treatment. We also found that the 35S plants maintained significantly higher photosynthetic capacity, and elevated activity of Rubisco and sucrose-phosphate synthase under heat stress. These results suggest that improvement of heat stress tolerance in transgenic chrysanthemum may be associated with enhanced tolerance of photosynthesis.
Plant Molecular Biology – Springer Journals
Published: Feb 21, 2009
It’s your single place to instantly
discover and read the research
that matters to you.
Enjoy affordable access to
over 18 million articles from more than
15,000 peer-reviewed journals.
All for just $49/month
Query the DeepDyve database, plus search all of PubMed and Google Scholar seamlessly
Save any article or search result from DeepDyve, PubMed, and Google Scholar... all in one place.
Get unlimited, online access to over 18 million full-text articles from more than 15,000 scientific journals.
Read from thousands of the leading scholarly journals from SpringerNature, Elsevier, Wiley-Blackwell, Oxford University Press and more.
All the latest content is available, no embargo periods.
“Hi guys, I cannot tell you how much I love this resource. Incredible. I really believe you've hit the nail on the head with this site in regards to solving the research-purchase issue.”Daniel C.
“Whoa! It’s like Spotify but for academic articles.”@Phil_Robichaud
“I must say, @deepdyve is a fabulous solution to the independent researcher's problem of #access to #information.”@deepthiw
“My last article couldn't be possible without the platform @deepdyve that makes journal papers cheaper.”@JoseServera