Key message A grafting response gene CcPIP1;2 was cloned from hickory plant, further functional characterization of the gene for water transport activity and abiotic stress tolerances were carried out through heterologous expres- sion in Xenopus and Arabidopsis. Abstract Plasma membrane intrinsic proteins (PIPs) are multifunctional channel proteins belonging to the membrane intrin- sic protein (MIP) family. In this study, a grafting-responsive gene from hickory (CcPIP1;2) was cloned and functionally characterized. Application of non-selective water inhibitors (HgCl and phloretin) led to the death of grafted hickory plants at 30 days after grafting (DAG). Furthermore, the transcript accumulation of the selected CcPIP1;2 gene was gradually decreased from 0 to 14 DAG in the grafted samples under inhibitor treatment conditions. Transient expression analysis of the GFP-CcPIP1;2 fusion protein showed that CcPIP1;2 was located at plasma membrane. Heterologous expression of CcPIP1;2 protein in the Xenopus oocyte system helped the access of water into the cells. Over-expression of CcPIP1;2 in Arabidopsis improved the percentage of seed germination when the seeds were grown in H O -, ABA-, and mannitol-containing media, 2 2 but had no effect when grown in the salt containing media. CcPIP1;2 transgenic plants grew better under drought conditions. The expression of
Plant Cell Reports – Springer Journals
Published: Jan 15, 2018
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