Osteogain® loaded onto an absorbable collagen sponge induces attachment and osteoblast differentiation of ST2 cells in vitro

Osteogain® loaded onto an absorbable collagen sponge induces attachment and osteoblast... Clin Oral Invest (2017) 21:2265–2272 DOI 10.1007/s00784-016-2019-5 ORIGINAL ARTICLE Osteogain® loaded onto an absorbable collagen sponge induces attachment and osteoblast differentiation of ST2 cells in vitro 1,2,3 1,4,5 6 Richard J. Miron & Masako Fujioka-Kobayashi & Yufeng Zhang & 7 8 9 10 Anton Sculean & Benjamin Pippenger & Yoshinori Shirakata & Umadevi Kandalam & Maria Hernandez Received: 5 October 2016 /Accepted: 23 November 2016 /Published online: 1 December 2016 Springer-Verlag Berlin Heidelberg 2016 Abstract matrix derivative (EMD) in combination with an absorbable Objectives Dimensional changes of the alveolar bone follow- collagen sponge (ACS) specifically designed for extraction ing tooth extraction are a major challenge in daily dental prac- socket healing. tice. To limit bone loss, a variety of biomaterials including Materials and methods The potential of ACS was first inves- bone grafts, barrier membranes, and growth factors have been tigated using ELISA to quantify total amelogenin adsorption utilized either alone or in combination therapies to increase the and release from 0 to 10 days. Thereafter, the cellular effects speed and quality of new bone formation. The aim of the of ST2 pre-osteoblasts were investigated for cellular attach- present in vitro study was to investigate the regenerative po- ment http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Clinical Oral Investigations Springer Journals

Osteogain® loaded onto an absorbable collagen sponge induces attachment and osteoblast differentiation of ST2 cells in vitro

Osteogain® loaded onto an absorbable collagen sponge induces attachment and osteoblast differentiation of ST2 cells in vitro

Clin Oral Invest (2017) 21:2265–2272 DOI 10.1007/s00784-016-2019-5 ORIGINAL ARTICLE Osteogain® loaded onto an absorbable collagen sponge induces attachment and osteoblast differentiation of ST2 cells in vitro 1,2,3 1,4,5 6 Richard J. Miron & Masako Fujioka-Kobayashi & Yufeng Zhang & 7 8 9 10 Anton Sculean & Benjamin Pippenger & Yoshinori Shirakata & Umadevi Kandalam & Maria Hernandez Received: 5 October 2016 /Accepted: 23 November 2016 /Published online: 1 December 2016 Springer-Verlag Berlin Heidelberg 2016 Abstract matrix derivative (EMD) in combination with an absorbable Objectives Dimensional changes of the alveolar bone follow- collagen sponge (ACS) specifically designed for extraction ing tooth extraction are a major challenge in daily dental prac- socket healing. tice. To limit bone loss, a variety of biomaterials including Materials and methods The potential of ACS was first inves- bone grafts, barrier membranes, and growth factors have been tigated using ELISA to quantify total amelogenin adsorption utilized either alone or in combination therapies to increase the and release from 0 to 10 days. Thereafter, the cellular effects speed and quality of new bone formation. The aim of the of ST2 pre-osteoblasts were investigated for cellular attach- present in vitro study was to investigate the regenerative po- ment at 8 h and cell proliferation at 1, 3, and 5 days as well as tential of Osteogain®, a new liquid carrier system of enamel osteoblast differentiation by real-time PCR and alizarin red staining for cells seeded on (1) tissue culture plastic, (2) ACS alone, and (3) ACS + Osteogain®. * Richard J. Miron Results ACS efficiently loaded nearly 100% of the rmiron@nova.edu amelogenin proteins found in Osteogain® which were gradu- ally released up to a 10-day period. Osteogain® also signifi- cantly induced a 1.5-fold increase in cell attachment and re- Department of Periodontology, College of Dental...
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Publisher
Springer Berlin Heidelberg
Copyright
Copyright © 2016 by Springer-Verlag Berlin Heidelberg
Subject
Dentistry; Dentistry
ISSN
1432-6981
eISSN
1436-3771
D.O.I.
10.1007/s00784-016-2019-5
Publisher site
See Article on Publisher Site

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