OsPIPK1, a Rice Phosphatidylinositol Monophosphate Kinase, Regulates Rice Heading by Modifying the Expression of Floral Induction Genes

OsPIPK1, a Rice Phosphatidylinositol Monophosphate Kinase, Regulates Rice Heading by Modifying... A rice gene, OsPIPK1, encoding a 792-aa putative phosphatidylinositol 4-phosphate 5-kinase (PIPK), was identified and characterized. Comparison between the cDNA and genomic sequences revealed the presence of 10 exons (39–1050 bp) and 9 introns (88–745 bp) in OsPIPK1 gene. The deduced amino acid sequence of OsPIPK1 contains a lipid kinase domain that is highly homologous to those of previously isolated PIPKs, and structural analysis revealed the intriguing presence of multiple MORN motifs at the N-terminus. The MORN motifs have also been detected in PIPKs from Arabidopsis thaliana and Oryza sativa, but not in the well-characterized PIPKs from animal and yeast cells. RT-PCR analysis indicated that OsPIPK1 was expressed almost constitutively in roots, shoots, stems, leaves and flowers, and up-regulated following treatment with plant hormones or application of various stresses. An antisense transgenic strategy was used to suppress the expression of OsPIPK1, and homozygous transgenic plants showed earlier heading (7–14 days earlier) than control plants, suggesting that OsPIPK1 negatively regulates floral initiation. This was further confirmed by morphologic observation showing earlier floral development in antisense plants, as well as leaf emergence measurement indicating delayed leaf development under OsPIPK1 deficiency, a common phenotype observed with earlier flowering. RT-PCR analysis and cDNA chip technology were used to examine transcripts of various genes in the transgenic plants and the results showed altered transcriptions of several flowering-time or -identity related genes, suggesting that OsPIPK1 is involved in rice heading through regulation of floral induction genes, signaling and metabolic pathways. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Plant Molecular Biology Springer Journals

OsPIPK1, a Rice Phosphatidylinositol Monophosphate Kinase, Regulates Rice Heading by Modifying the Expression of Floral Induction Genes

Loading next page...
 
/lp/springer_journal/ospipk1-a-rice-phosphatidylinositol-monophosphate-kinase-regulates-wEcYydBt8Z
Publisher
Kluwer Academic Publishers
Copyright
Copyright © 2004 by Kluwer Academic Publishers
Subject
Life Sciences; Biochemistry, general; Plant Sciences; Plant Pathology
ISSN
0167-4412
eISSN
1573-5028
D.O.I.
10.1023/B:PLAN.0000028796.14336.24
Publisher site
See Article on Publisher Site

Abstract

A rice gene, OsPIPK1, encoding a 792-aa putative phosphatidylinositol 4-phosphate 5-kinase (PIPK), was identified and characterized. Comparison between the cDNA and genomic sequences revealed the presence of 10 exons (39–1050 bp) and 9 introns (88–745 bp) in OsPIPK1 gene. The deduced amino acid sequence of OsPIPK1 contains a lipid kinase domain that is highly homologous to those of previously isolated PIPKs, and structural analysis revealed the intriguing presence of multiple MORN motifs at the N-terminus. The MORN motifs have also been detected in PIPKs from Arabidopsis thaliana and Oryza sativa, but not in the well-characterized PIPKs from animal and yeast cells. RT-PCR analysis indicated that OsPIPK1 was expressed almost constitutively in roots, shoots, stems, leaves and flowers, and up-regulated following treatment with plant hormones or application of various stresses. An antisense transgenic strategy was used to suppress the expression of OsPIPK1, and homozygous transgenic plants showed earlier heading (7–14 days earlier) than control plants, suggesting that OsPIPK1 negatively regulates floral initiation. This was further confirmed by morphologic observation showing earlier floral development in antisense plants, as well as leaf emergence measurement indicating delayed leaf development under OsPIPK1 deficiency, a common phenotype observed with earlier flowering. RT-PCR analysis and cDNA chip technology were used to examine transcripts of various genes in the transgenic plants and the results showed altered transcriptions of several flowering-time or -identity related genes, suggesting that OsPIPK1 is involved in rice heading through regulation of floral induction genes, signaling and metabolic pathways.

Journal

Plant Molecular BiologySpringer Journals

Published: Oct 18, 2004

References

You’re reading a free preview. Subscribe to read the entire article.


DeepDyve is your
personal research library

It’s your single place to instantly
discover and read the research
that matters to you.

Enjoy affordable access to
over 18 million articles from more than
15,000 peer-reviewed journals.

All for just $49/month

Explore the DeepDyve Library

Search

Query the DeepDyve database, plus search all of PubMed and Google Scholar seamlessly

Organize

Save any article or search result from DeepDyve, PubMed, and Google Scholar... all in one place.

Access

Get unlimited, online access to over 18 million full-text articles from more than 15,000 scientific journals.

Your journals are on DeepDyve

Read from thousands of the leading scholarly journals from SpringerNature, Elsevier, Wiley-Blackwell, Oxford University Press and more.

All the latest content is available, no embargo periods.

See the journals in your area

DeepDyve

Freelancer

DeepDyve

Pro

Price

FREE

$49/month
$360/year

Save searches from
Google Scholar,
PubMed

Create lists to
organize your research

Export lists, citations

Read DeepDyve articles

Abstract access only

Unlimited access to over
18 million full-text articles

Print

20 pages / month

PDF Discount

20% off