OsDMC1 is required for homologous pairing in Oryza sativa

OsDMC1 is required for homologous pairing in Oryza sativa OsDMC1 is the rice homologue of the yeast DMC1 gene. Here, we analyzed the function of OsDMC1 in meiosis using an RNA interference approach. The OsDMC1–RNAi lines grew normally during their vegetative phase but showed spikelet and pollen sterility. The sterility phenotypes were associated with down-regulated OsDMC1 transcript and protein levels mediated by RNAi. Further cytological observations of male meiocytes revealed that knock-down of OsDMC1 led to defects in bivalent formation and subsequent unequal chromosome segregation and irregular spore generation, and induced changes in male meiotic progression. Fluorescent in situ hybridization experiments revealed that the OsDMC1–RNAi lines were defective in homologous pairing. These data indicate that OsDMC1 is essential for rice meiosis and plays an important role in homologous pairing. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Plant Molecular Biology Springer Journals

OsDMC1 is required for homologous pairing in Oryza sativa

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Publisher
Springer Netherlands
Copyright
Copyright © 2007 by Springer Science+Business Media B.V.
Subject
Life Sciences; Plant Pathology; Biochemistry, general; Plant Sciences
ISSN
0167-4412
eISSN
1573-5028
D.O.I.
10.1007/s11103-007-9195-2
Publisher site
See Article on Publisher Site

Abstract

OsDMC1 is the rice homologue of the yeast DMC1 gene. Here, we analyzed the function of OsDMC1 in meiosis using an RNA interference approach. The OsDMC1–RNAi lines grew normally during their vegetative phase but showed spikelet and pollen sterility. The sterility phenotypes were associated with down-regulated OsDMC1 transcript and protein levels mediated by RNAi. Further cytological observations of male meiocytes revealed that knock-down of OsDMC1 led to defects in bivalent formation and subsequent unequal chromosome segregation and irregular spore generation, and induced changes in male meiotic progression. Fluorescent in situ hybridization experiments revealed that the OsDMC1–RNAi lines were defective in homologous pairing. These data indicate that OsDMC1 is essential for rice meiosis and plays an important role in homologous pairing.

Journal

Plant Molecular BiologySpringer Journals

Published: Jun 12, 2007

References

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